Construct: sgRNA BRDN0001149093

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTGACCTTGAGATCACAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK1 (5594)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77788

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000022.11 22 21805861 + MAPK1 NNNGACCTTGAGATCACAGG NGG 0 1.0 Tier I
2 NC_000014.9 14 64158779 + SYNE2 NNNGACCTTGAGATGCCAGG NGG 2 0.0 Tier I
3 NC_000014.9 14 69920225 - SMOC1 NNNGACCTAGAGATCACAGA NGG 2 0.8705 Tier II
4 NC_000012.12 12 6338346 - TNFRSF1A NNNGACCGAGAGATCACAGG NGG 2 0.681 Tier II
5 NC_000006.12 6 42254738 + TRERF1 NNNGGCCTTGAGATCACAAG NGG 2 0.5143 Tier II
6 NC_000005.10 5 172908083 - ERGIC1 NNNGACCTAGACATCACAGG NGG 2 0.4916 Tier II
7 NC_000009.12 9 129105732 - CRAT NNNGACCTTGAGATCAAAGT NGG 2 0.3267 Tier II
8 NC_000009.12 9 135663897 + LCN9 NNNGACCTTGGGATCAAAGG NGG 2 0.3033 Tier II
9 NC_000006.12 6 36331917 - BNIP5 NNNGACCTAGAGACCACAGG NGG 2 0.2653 Tier II
10 NC_000002.12 2 54218765 + ACYP2 NNNGTCCTTGAGATCACATG NGG 2 0.2424 Tier II
11 NC_000003.12 3 50786381 - DOCK3 NNNGACCTTGAGCTCACAGA NGG 2 0.1974 Tier II
12 NC_000022.11 22 29329391 + AP1B1 NNNGAGCTTGAGATCACAGG NAG 2 0.1296 Tier II
13 NC_000012.12 12 64810098 - TBC1D30 NNNGGCCTTGAGATCACAGG NGA 2 0.05 Tier II
14 NC_000016.10 16 16111364 - ABCC1 NNNGACCTGGAGATCACAGG NGA 2 0.043 Tier II
15 NC_000019.10 19 9225583 + OR7E24 NNNGACCTTGAGATGACAGG NAG 2 0.013 Tier II
16 NC_000010.11 10 71422661 - CDH23 NNNGGCCTTGAGATCCCAGG NGG 2 0.0 Tier II
17 NC_000006.12 6 42254738 + LOC124901316 NNNGGCCTTGAGATCACAAG NGG 2 0.5143 Tier III
18 NC_000017.11 17 21217438 + TMEM11-DT NNNGACTTGGAGATCACAGG NGG 2 0.503 Tier III
19 NC_000005.10 5 12598065 - LINC01194 NNNGACCTGGAGAACACAGG NGG 2 0.3832 Tier III
20 NC_000016.10 16 35144069 - LOC105371197 NNNGTCCTTGAGATCACAGG NGG 1 0.3636 Tier III
21 NC_000016.10 16 35140984 - LOC105371197 NNNGTCCTCGAGATCACAGG NGG 2 0.3357 Tier III
22 NC_000006.12 6 88403050 - LOC105377885 NNNGACCATGAGACCACAGG NGG 2 0.2286 Tier III
23 NC_000003.12 3 50786381 - ZNF652P1 NNNGACCTTGAGCTCACAGA NGG 2 0.1974 Tier III
24 NC_000012.12 12 46491203 - SLC38A4-AS1 NNNTACCTTGAGATCACTGG NGG 2 0.1818 Tier III
25 NC_000012.12 12 46491203 - LOC124902923 NNNTACCTTGAGATCACTGG NGG 2 0.1818 Tier III
26 NC_000016.10 16 80071436 + LOC105371357 NNNGACCTTGAGCTCACTGG NGG 2 0.1053 Tier III
27 NC_000017.11 17 45881166 - MAPT-AS1 NNNGACCTTGAGATGACGGG NGG 2 0.02 Tier III
28 NC_000013.11 13 43138607 - LINC00400 NNNGACCTTGAGATCCCAGG NGC 2 0.0 Tier III
29 NC_000005.10 5 24151386 - LINC02899 NNNGACCTTTAGAGCACAGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 17018465 - Mapk1 NNNGACCTTGAGATCACAAG NGG 1 0.7143 Tier I
2 NC_000071.6 5 96740848 - Fras1 NNNGACCATGACATCACAGG NGG 2 0.4235 Tier I
3 NC_000073.6 7 138843827 + Mapk1ip1 NNNGACCTTAAGATCACAGA NGG 2 0.875 Tier II
4 NC_000074.6 8 104377286 + Cmtm4 NNNGGCCTTGAAATCACAGG NGG 2 0.672 Tier II
5 NC_000070.6 4 140734399 - Padi6 NNNGACCTTGAAATCACAGT NGG 2 0.6533 Tier II
6 NC_000068.7 2 73664885 - Chn1 NNNGACCTTGAGATCAAAGA NGG 2 0.4375 Tier II
7 NC_000067.6 1 151919442 + 1700025G04Rik NNNGGCCTTGATATCACAGG NGG 2 0.2769 Tier II
8 NC_000075.6 9 26837794 + Glb1l3 NNNGACCTTGGGATCACGGG NGG 2 0.26 Tier II
9 NC_000074.6 8 14246807 + Dlgap2 NNNGACCATGAGATCACAGG NAG 2 0.2074 Tier II
10 NC_000068.7 2 103722662 + Nat10 NNNGACCTAGAGCTCACAGG NGG 2 0.1955 Tier II
11 NC_000067.6 1 43146643 + Fhl2 NNNAACCTTGAGCTCACAGG NGG 2 0.1895 Tier II
12 NC_000076.6 10 83152948 - Chst11 NNNGACCTTGATACCACAGG NGG 2 0.1099 Tier II
13 NC_000081.6 15 72937778 + Trappc9 NNNGATCTTGAGATGACAGG NGG 2 0.0464 Tier II
14 NC_000079.6 13 52620642 - Syk NNNGACCTCGAGATGACAGG NGG 2 0.0462 Tier II
15 NC_000067.6 1 123908681 - Dpp10 NNNCACCTTGAGATCACAGG NGA 2 0.0368 Tier II
16 NC_000072.6 6 64303483 - Grid2 NNNGACCTTGAGATCACTGG NGA 2 0.0347 Tier II
17 NC_000073.6 7 56782480 + Gabrg3 NNNGACCTTGAGATCACTGG NGA 2 0.0347 Tier II
18 NC_000075.6 9 4548524 + Gria4 NNNGACCTTGAGATCACTGG NGA 2 0.0347 Tier II
19 NC_000075.6 9 10172821 + Cntn5 NNNGACCTTGAGATCACTGG NGA 2 0.0347 Tier II
20 NC_000075.6 9 10173044 + Cntn5 NNNGACCTTGAGATCACTGG NGA 2 0.0347 Tier II
21 NC_000075.6 9 24566919 + Dpy19l2 NNNGACCTTGAGATCACTGG NGA 2 0.0347 Tier II
22 NC_000080.6 14 18855536 + Ube2e2 NNNGACCTTGAGATCACTGG NGA 2 0.0347 Tier II
23 NC_000082.6 16 16818729 - Spag6l NNNGACCTTGAGATCACTGG NGA 2 0.0347 Tier II
24 NC_000085.6 19 39625276 - Cyp2c67 NNNGACCTTGAGATCACTGG NGA 2 0.0347 Tier II
25 NC_000076.6 10 85551589 - Btbd11 NNNGCCCTTGAGATCACAGG NGA 2 0.0347 Tier II
26 NC_000080.6 14 16301309 - Ngly1 NNNGACCTTGTGATCACAGG NGA 2 0.0214 Tier II
27 NC_000083.6 17 21950210 - Zfp942 NNNGACCTTGTGATCACAGG NGA 2 0.0214 Tier II
28 NC_000076.6 10 87547974 + Pah NNNAACCTTGAGATCACAGG NGC 2 0.02 Tier II
29 NC_000079.6 13 57534798 - Spock1 NNNGACCTTGAGATCACACG NTG 2 0.0175 Tier II
30 NC_000083.6 17 50137329 + Rftn1 NNNCACCTTGAGATCCCAGG NGG 2 0.0 Tier II
31 NC_000073.6 7 74548147 - Slco3a1 NNNGACCTTGAGATGTCAGG NGG 2 0.0 Tier II
32 NC_000081.6 15 76673003 - Ppp1r16a NNNGAGCTTGAGAGCACAGG NGG 2 0.0 Tier II
33 NC_000072.6 6 67944783 + Igk NNNGATCTTGAGATCACTGG NGG 2 0.4643 Tier III
34 NC_000078.6 12 7646320 - Gm32828 NNNGACCTTGGGATCACAGT NGG 2 0.455 Tier III
35 NC_000069.6 3 15057091 + Gm42000 NNNGACCTTGAGATCACAGA NAG 2 0.2431 Tier III
36 NC_000067.6 1 132277114 - Gm29630 NNNGACCTTGAGATCATAGC NGG 2 0.2 Tier III
37 NC_000078.6 12 15672402 - Gm40287 NNNGACCTTGTGATCACAGG NGA 2 0.0214 Tier III
38 NC_000071.6 5 120913939 + Gm7573 NNNGACATTGAGATCACAGG NGT 2 0.0121 Tier III
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Other clones with same target sequence:

(none)