Construct: sgRNA BRDN0001149097
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGCGTAAGACCCCAGCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TK2 (7084)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77299
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
66531445 |
+ |
TK2 |
NNNCGTAAGACCCCAGCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
67089415 |
+ |
PCDH9 |
NNNCGTAAGACTCCAGCGAA |
NGG |
2 |
0.5048 |
Tier II |
| 3 |
NC_000004.12 |
4 |
56598955 |
- |
SPMAP2L |
NNNCGTAAGAGCCCAGCGAA |
NGG |
2 |
0.2344 |
Tier II |
| 4 |
NC_000016.10 |
16 |
88268253 |
- |
ZNF469 |
NNNCTTAAGACACCAGCGAG |
NGG |
2 |
0.2143 |
Tier II |
| 5 |
NC_000016.10 |
16 |
70985764 |
+ |
HYDIN |
NNNCGTAAGGCCCCAGCGAG |
NTG |
2 |
0.0216 |
Tier II |
| 6 |
NC_000002.12 |
2 |
740585 |
+ |
LOC105373480 |
NNNCGTTAGAGCCCAGCGAG |
NGG |
2 |
0.1094 |
Tier III |
| 7 |
NC_000002.12 |
2 |
740774 |
+ |
LOC105373480 |
NNNCGTTAGAGCCCAGCGAG |
NGG |
2 |
0.1094 |
Tier III |
| 8 |
NC_000009.12 |
9 |
36826517 |
- |
LOC105376030 |
NNNTGTAAGACCCCAGGGAG |
NGG |
2 |
0.0471 |
Tier III |
| 9 |
NC_000001.11 |
1 |
146731592 |
- |
HYDIN2 |
NNNCGTAAGGCCCCAGCGAG |
NTG |
2 |
0.0216 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
83719157 |
- |
Scube1 |
NNNTGTGAGACCCCAGCGAG |
NGG |
2 |
0.5647 |
Tier II |
| 2 |
NC_000071.6 |
5 |
45763980 |
+ |
Lcorl |
NNNCGTAAGACCCCAGAGAA |
NGG |
2 |
0.4375 |
Tier II |
| 3 |
NC_000070.6 |
4 |
114730535 |
- |
Gm33655 |
NNNTGAAAGACCCCAGCGAG |
NGG |
2 |
0.6933 |
Tier III |
Other clones with same target sequence:
(none)
