Construct: sgRNA BRDN0001149099

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCGGACACGCAAATTTGTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK10 (6793)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75488

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 172090317 - STK10 NNNGACACGCAAATTTGTGG NGG 0 1.0 Tier I
2 NC_000011.10 11 47133314 + CSTPP1 NNNGACACACAAATTTGTGG NGA 2 0.0446 Tier II
3 NC_000007.14 7 71697418 + GALNT17 NNNGACATGCAAATTTGTGG NTG 2 0.0341 Tier II
4 NC_000001.11 1 42569121 + CCDC30 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
5 NC_000006.12 6 82210357 - IBTK NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
6 NC_000011.10 11 16974440 - PLEKHA7 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
7 NC_000011.10 11 88612824 - GRM5 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
8 NC_000001.11 1 19305095 - AKR7A2 NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier II
9 NC_000012.12 12 51014153 - SLC11A2 NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier II
10 NC_000006.12 6 18306992 - RNU6-263P NNNGACACGCAAATTTGTGA NGG 1 0.9375 Tier III
11 NC_000006.12 6 31083091 + RNU6-1133P NNNGACACACAAATTTGTGA NGG 2 0.6027 Tier III
12 NC_000019.10 19 20240960 - LOC105372310 NNNGACAGGCGAATTTGTGG NGG 2 0.4179 Tier III
13 NC_000015.10 15 93651945 + LOC107983974 NNNGACCCCCAAATTTGTGG NGG 2 0.2356 Tier III
14 NC_000011.10 11 47133314 + LOC124902671 NNNGACACACAAATTTGTGG NGA 2 0.0446 Tier III
15 NC_000001.11 1 42569121 + RNU6-536P NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
16 NC_000006.12 6 82210357 - RNU6-130P NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
17 NC_000011.10 11 16974440 - LOC124902817 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
18 NC_000011.10 11 88612824 - RNU6-16P NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
19 NC_000014.9 14 68735941 - LOC105370547 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
20 NC_000014.9 14 68735941 - RNU6-921P NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
21 NC_000001.11 1 19305095 - RNU6-1099P NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
22 NC_000001.11 1 19305095 - LOC124903867 NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
23 NC_000006.12 6 117457753 - RNU6-253P NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
24 NC_000008.11 8 137105440 + RNU6-144P NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
25 NC_000012.12 12 51014153 - RNU6-87P NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
26 NC_000017.11 17 76656404 + RNU6-227P NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
27 NC_000003.12 3 70808434 + RNU6-281P NNNGACACGCAAATTTGTGG NAC 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 47732363 + Sfr1 NNNGAAACGCAAATTTTTGG NGG 2 0.2321 Tier II
2 NC_000069.6 3 106691092 - Lrif1 NNNGACACTCAAACTTGTGG NGG 2 0.1524 Tier II
3 NC_000086.7 X 85783244 - 5430427O19Rik NNNGACACACAAATTTGTGG NTG 2 0.025 Tier II
4 NC_000068.7 2 25006171 - Pnpla7 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
5 NC_000069.6 3 24562162 + Naaladl2 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
6 NC_000071.6 5 72418732 - Corin NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
7 NC_000071.6 5 110677143 - Ep400 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
8 NC_000071.6 5 139443687 + 3110082I17Rik NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
9 NC_000075.6 9 32446841 - Fli1 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
10 NC_000077.6 11 5251938 + Kremen1 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
11 NC_000080.6 14 75651442 - Erich6b NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier II
12 NC_000067.6 1 124490941 + Dpp10 NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier II
13 NC_000082.6 16 91921328 - Gm25245 NNNGACACGCAAATTCGTGA NGG 2 0.625 Tier III
14 NC_000086.7 X 85783244 - Gm14766 NNNGACACACAAATTTGTGG NTG 2 0.025 Tier III
15 NC_000068.7 2 25006171 - Gm25518 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
16 NC_000069.6 3 24562162 + Gm24704 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
17 NC_000071.6 5 72418732 - Gm23712 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
18 NC_000071.6 5 110677143 - Gm22716 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
19 NC_000071.6 5 139443687 + Gm26285 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
20 NC_000075.6 9 32446841 - Gm22060 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
21 NC_000077.6 11 5251938 + Gm25142 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
22 NC_000078.6 12 80911565 + Gm23298 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
23 NC_000080.6 14 75651442 - Gm23622 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
24 NC_000081.6 15 80088466 - Gm24204 NNNGACACGCAAATTTGTGA NGC 2 0.0208 Tier III
25 NC_000067.6 1 124490941 + Gm25578 NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
26 NC_000068.7 2 133584334 - Gm25258 NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
27 NC_000071.6 5 59214965 - Gm22308 NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
28 NC_000073.6 7 130035185 + Gm23847 NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
29 NC_000074.6 8 79014383 - Gm22044 NNNGACACGCAAATTTGTGA NGT 2 0.0151 Tier III
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Other clones with same target sequence:

(none)