Construct: sgRNA BRDN0001149105
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTGATGCCATACCATGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROS1 (6098)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75992
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
117344157 |
+ |
ROS1 |
NNNTGATGCCATACCATGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
30411589 |
+ |
MPPED2 |
NNNTGATGCCATAACCTGTG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000006.12 |
6 |
55215680 |
+ |
HCRTR2 |
NNNTGATAACATACCATGTG |
NGG |
2 |
0.8571 |
Tier II |
| 4 |
NC_000012.12 |
12 |
11037010 |
- |
PRH1 |
NNNTGATGAAATACCATGTG |
NGG |
2 |
0.7429 |
Tier II |
| 5 |
NC_000012.12 |
12 |
11037010 |
- |
PRH1-TAS2R14 |
NNNTGATGAAATACCATGTG |
NGG |
2 |
0.7429 |
Tier II |
| 6 |
NC_000012.12 |
12 |
11097914 |
- |
PRH1 |
NNNTGATGAAATACCATGTG |
NGG |
2 |
0.7429 |
Tier II |
| 7 |
NC_000012.12 |
12 |
11097914 |
- |
PRH1-TAS2R14 |
NNNTGATGAAATACCATGTG |
NGG |
2 |
0.7429 |
Tier II |
| 8 |
NC_000009.12 |
9 |
126474831 |
- |
MVB12B |
NNNTGAAGCCAGACCATGTG |
NGG |
2 |
0.4375 |
Tier II |
| 9 |
NC_000008.11 |
8 |
31783816 |
- |
NRG1 |
NNNTGATACCATACCATGCG |
NGG |
2 |
0.2759 |
Tier II |
| 10 |
NC_000009.12 |
9 |
72675442 |
+ |
TMC1 |
NNNTGATTCCATCCCATGTG |
NGG |
2 |
0.1316 |
Tier II |
| 11 |
NC_000015.10 |
15 |
76261711 |
+ |
ETFA |
NNNTGATGCCATACCATGCC |
NGG |
2 |
0.1182 |
Tier II |
| 12 |
NC_000015.10 |
15 |
25390055 |
- |
UBE3A |
NNNTGATGCCATATGATGTG |
NGG |
2 |
0.0367 |
Tier II |
| 13 |
NC_000008.11 |
8 |
14709825 |
+ |
SGCZ |
NNNTGATGCCTTACTATGTG |
NGG |
2 |
0.0205 |
Tier II |
| 14 |
NC_000013.11 |
13 |
33173522 |
- |
STARD13 |
NNNTGATGCCATACCATGGG |
NTG |
2 |
0.0097 |
Tier II |
| 15 |
NC_000002.12 |
2 |
100401472 |
- |
CHST10 |
NNNGGATGCCATACCCTGTG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000006.12 |
6 |
13430516 |
- |
GFOD1 |
NNNTGATGCCATACCCTATG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000012.12 |
12 |
11037010 |
- |
PRH1-PRR4 |
NNNTGATGAAATACCATGTG |
NGG |
2 |
0.7429 |
Tier III |
| 18 |
NC_000012.12 |
12 |
11097914 |
- |
PRH1-PRR4 |
NNNTGATGAAATACCATGTG |
NGG |
2 |
0.7429 |
Tier III |
| 19 |
NC_000015.10 |
15 |
25390055 |
- |
SNHG14 |
NNNTGATGCCATATGATGTG |
NGG |
2 |
0.0367 |
Tier III |
| 20 |
NC_000004.12 |
4 |
27264215 |
+ |
LINC02261 |
NNNTGATGCCATACCAGGTG |
NTG |
2 |
0.0046 |
Tier III |
| 21 |
NC_000004.12 |
4 |
27264215 |
+ |
LOC124900842 |
NNNTGATGCCATACCAGGTG |
NTG |
2 |
0.0046 |
Tier III |
| 22 |
NC_000001.11 |
1 |
115363822 |
- |
LOC112268234 |
NNNTGAGGCCATACCCTGTG |
NGG |
2 |
0.0 |
Tier III |
| 23 |
NC_000001.11 |
1 |
115363822 |
- |
NGF-AS1 |
NNNTGAGGCCATACCCTGTG |
NGG |
2 |
0.0 |
Tier III |
| 24 |
NC_000013.11 |
13 |
19298254 |
- |
ANKRD26P3 |
NNNTGATGCCCTACCTTGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
116697654 |
- |
Slc35a3 |
NNNTGATGTTATACCATGTG |
NGG |
2 |
0.8235 |
Tier II |
| 2 |
NC_000069.6 |
3 |
125925409 |
- |
Ugt8a |
NNNTGAAGTCATACCATGTG |
NGG |
2 |
0.7656 |
Tier II |
| 3 |
NC_000075.6 |
9 |
113952208 |
+ |
Ubp1 |
NNNTGATGCAATGCCATGTG |
NGG |
2 |
0.5652 |
Tier II |
| 4 |
NC_000070.6 |
4 |
108072058 |
+ |
Scp2 |
NNNTGATGCCATACCACTTG |
NGG |
2 |
0.4706 |
Tier II |
| 5 |
NC_000083.6 |
17 |
84633205 |
- |
Dync2li1 |
NNNTGATCCCATGCCATGTG |
NGG |
2 |
0.4013 |
Tier II |
| 6 |
NC_000072.6 |
6 |
83039924 |
+ |
Loxl3 |
NNNTGATGCCCCACCATGTG |
NGG |
2 |
0.3789 |
Tier II |
| 7 |
NC_000076.6 |
10 |
32306380 |
- |
Nkain2 |
NNNTGAGTCCATACCATGTG |
NGG |
2 |
0.3676 |
Tier II |
| 8 |
NC_000077.6 |
11 |
59156355 |
+ |
Iba57 |
NNNTGGTGCCTTACCATGTG |
NGG |
2 |
0.2198 |
Tier II |
| 9 |
NC_000075.6 |
9 |
56626211 |
- |
Lingo1 |
NNNGGATGCCATACAATGTG |
NGG |
2 |
0.1389 |
Tier II |
| 10 |
NC_000076.6 |
10 |
44145489 |
- |
Crybg1 |
NNNTGATGCCAGACCATGGG |
NGG |
2 |
0.125 |
Tier II |
| 11 |
NC_000078.6 |
12 |
29744094 |
- |
Myt1l |
NNNTCATGCCATACCATGTG |
NGA |
2 |
0.0546 |
Tier II |
| 12 |
NC_000077.6 |
11 |
109957370 |
- |
Abca8b |
NNNCGATGCCATACGATGTG |
NGG |
2 |
0.0324 |
Tier II |
| 13 |
NC_000072.6 |
6 |
5010465 |
- |
Ppp1r9a |
NNNTGATGCCAGACCATGTG |
NGT |
2 |
0.0081 |
Tier II |
| 14 |
NC_000077.6 |
11 |
100602158 |
- |
Dnajc7 |
NNNTGATACCATACCTTGTG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000085.6 |
19 |
52960224 |
- |
Xpnpep1 |
NNNTGATGCCATCCCCTGTG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000078.6 |
12 |
81726033 |
- |
Map3k9 |
NNNTGATTCCATAGCATGTG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000069.6 |
3 |
153148066 |
+ |
4930482G09Rik |
NNNTGTTGCTATACCATGTG |
NGG |
2 |
0.6723 |
Tier III |
Other clones with same target sequence:
(none)
