Construct: sgRNA BRDN0001149107

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGTCAATGATCTTGATCCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK1 (1612)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76520

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 87639399 - DAPK1 NNNTCAATGATCTTGATCCG NGG 0 1.0 Tier I
2 NC_000014.9 14 50470059 - MAP4K5 NNNTCAATTATCTTGCTCCG NGG 2 0.0 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 60718059 - Dapk1 NNNTCTATGATCTTGATCCG NGG 1 0.7143 Tier I
2 NC_000086.7 X 12089206 + Bcor NNNTCAATGACCTTGATCCT NGG 2 0.4667 Tier II
3 NC_000083.6 17 85456503 + Camkmt NNNTCAATGATCTTGTTCCG NTG 2 0.0 Tier II
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Other clones with same target sequence:

(none)