Construct: sgRNA BRDN0001149109
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACGAGCTGCCAGTGAACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CKMT1A (548596)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75880
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
43594345 |
+ |
CKMT1B |
NNNGAGCTGCCAGTGAACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
43694177 |
+ |
CKMT1A |
NNNGAGCTGCCAGTGAACGA |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000002.12 |
2 |
182866128 |
- |
FRZB |
NNNGAGCTGCCAGTGTACGA |
NAG |
2 |
0.0 |
Tier I |
| 4 |
NC_000007.14 |
7 |
117405442 |
+ |
ASZ1 |
NNNGAGCTGCCAATGAACTA |
NGG |
2 |
0.6154 |
Tier II |
| 5 |
NC_000017.11 |
17 |
2149563 |
+ |
SMG6 |
NNNAAGCTACCAGTGAACGA |
NGG |
2 |
0.5786 |
Tier II |
| 6 |
NC_000003.12 |
3 |
31665527 |
+ |
OSBPL10 |
NNNGGGCTGCCAGTGAACTA |
NGG |
2 |
0.48 |
Tier II |
| 7 |
NC_000018.10 |
18 |
36997528 |
+ |
KIAA1328 |
NNNGAGCTGCCAGTAAACCA |
NGG |
2 |
0.4219 |
Tier II |
| 8 |
NC_000008.11 |
8 |
27901782 |
+ |
SCARA5 |
NNNGAGCTACCAGTGAAAGA |
NGG |
2 |
0.3462 |
Tier II |
| 9 |
NC_000012.12 |
12 |
11166321 |
- |
PRH1 |
NNNGATCTGCCAGTGAAGGA |
NGG |
2 |
0.0889 |
Tier II |
| 10 |
NC_000012.12 |
12 |
11166321 |
- |
PRH1-TAS2R14 |
NNNGATCTGCCAGTGAAGGA |
NGG |
2 |
0.0889 |
Tier II |
| 11 |
NC_000020.11 |
20 |
37240528 |
+ |
RPN2 |
NNNGAGCTGCCACTGAAGGA |
NGG |
2 |
0.0561 |
Tier II |
| 12 |
NC_000011.10 |
11 |
8643706 |
- |
TRIM66 |
NNNGAGCTGCCAGTCAACGA |
NGT |
2 |
0.0044 |
Tier II |
| 13 |
NC_000001.11 |
1 |
214008950 |
+ |
PROX1 |
NNNGAGCTGCCAGGGATCGA |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000006.12 |
6 |
3272228 |
+ |
SLC22A23 |
NNNGAGCTGCCTGTGCACGA |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000001.11 |
1 |
34470869 |
- |
LOC105378641 |
NNNGAGCTGCCAGAGAATGA |
NGG |
2 |
0.398 |
Tier III |
| 16 |
NC_000017.11 |
17 |
83119116 |
+ |
LOC101930496 |
NNNGAGCGGCCCGTGAACGA |
NGG |
2 |
0.193 |
Tier III |
| 17 |
NC_000013.11 |
13 |
111982197 |
- |
SOX1-OT |
NNNGGGCTGCCAGTGAAGGA |
NGG |
2 |
0.096 |
Tier III |
| 18 |
NC_000012.12 |
12 |
11166321 |
- |
PRH1-PRR4 |
NNNGATCTGCCAGTGAAGGA |
NGG |
2 |
0.0889 |
Tier III |
| 19 |
NC_000004.12 |
4 |
40318052 |
- |
LINC02265 |
NNNGAGCTGCCAGAGAAGGA |
NGG |
2 |
0.0825 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
13688284 |
+ |
Aig1 |
NNNGAGATGACAGTGAACGA |
NGG |
2 |
0.65 |
Tier II |
| 2 |
NC_000078.6 |
12 |
79806017 |
- |
Rad51b |
NNNGAGCTGCCAGTGAATGT |
NGG |
2 |
0.3857 |
Tier II |
| 3 |
NC_000074.6 |
8 |
84903552 |
+ |
Klf1 |
NNNGAGCCGCTAGTGAACGA |
NGG |
2 |
0.3077 |
Tier II |
| 4 |
NC_000067.6 |
1 |
172545467 |
- |
Cfap45 |
NNNGAGCTACCAGTGAACCA |
NGG |
2 |
0.2882 |
Tier II |
| 5 |
NC_000081.6 |
15 |
79372132 |
- |
Tmem184b |
NNNAAGCTGCCAGTGAGCGA |
NGG |
2 |
0.1588 |
Tier II |
| 6 |
NC_000070.6 |
4 |
149646590 |
+ |
Clstn1 |
NNNGAGCAGCCAGTGAGCGA |
NGG |
2 |
0.1412 |
Tier II |
| 7 |
NC_000075.6 |
9 |
50525658 |
- |
Pts |
NNNGAGCTGACAGTGAACGA |
NGA |
2 |
0.0602 |
Tier II |
| 8 |
NC_000082.6 |
16 |
9664710 |
+ |
Grin2a |
NNNGAGCTGCCAGTCAAGGA |
NGG |
2 |
0.0364 |
Tier II |
| 9 |
NC_000067.6 |
1 |
172545467 |
- |
4933439K11Rik |
NNNGAGCTACCAGTGAACCA |
NGG |
2 |
0.2882 |
Tier III |
Other clones with same target sequence:
(none)
