Construct: sgRNA BRDN0001149112
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTATCCCAGATAGAAGCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3C2B (5287)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76713
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
204469317 |
+ |
PIK3C2B |
NNNTCCCAGATAGAAGCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
54232319 |
+ |
ACYP2 |
NNNTCCCAGAAAGAAGCTCT |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000014.9 |
14 |
50231149 |
+ |
SOS2 |
NNNTCCCCGTTAGAAGCTCG |
NGG |
2 |
0.3782 |
Tier II |
| 4 |
NC_000019.10 |
19 |
54596127 |
+ |
LILRA1 |
NNNTCCCAGATAGAAGCCTG |
NGG |
2 |
0.1978 |
Tier II |
| 5 |
NC_000015.10 |
15 |
67125362 |
- |
SMAD3 |
NNNTCCCAGAGAGAAGATCG |
NGG |
2 |
0.1867 |
Tier II |
| 6 |
NC_000020.11 |
20 |
41518528 |
+ |
CHD6 |
NNNTCCCAGACAGAAGCTGG |
NGG |
2 |
0.0833 |
Tier II |
| 7 |
NC_000023.11 |
X |
150562938 |
+ |
MTM1 |
NNNTCCCAGAGAGAAGCTGG |
NGG |
2 |
0.05 |
Tier II |
| 8 |
NC_000011.10 |
11 |
19275864 |
- |
CSRP3-AS1 |
NNNTCCCAGGAAGAAGCTCG |
NGG |
2 |
0.4167 |
Tier III |
| 9 |
NC_000004.12 |
4 |
104592637 |
+ |
CXXC4-AS1 |
NNNTCCCAAATAGAAGCTAG |
NGG |
2 |
0.2755 |
Tier III |
| 10 |
NC_000004.12 |
4 |
104592637 |
+ |
LOC124900745 |
NNNTCCCAAATAGAAGCTAG |
NGG |
2 |
0.2755 |
Tier III |
| 11 |
NC_000016.10 |
16 |
2406548 |
- |
ABCA17P |
NNNTCACAGATAGAAGCTGG |
NGG |
2 |
0.1161 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
111258979 |
- |
Ttc28 |
NNNTCCCAGATAGGAGCTCT |
NGG |
2 |
0.3267 |
Tier II |
| 2 |
NC_000084.6 |
18 |
77516779 |
- |
Rnf165 |
NNNTGCCATATAGAAGCTCG |
NGG |
2 |
0.32 |
Tier II |
| 3 |
NC_000075.6 |
9 |
97014941 |
- |
Spsb4 |
NNNTCCCAGACAGAAGCTTG |
NGG |
2 |
0.3077 |
Tier II |
| 4 |
NC_000070.6 |
4 |
134744406 |
+ |
Ldlrap1 |
NNNCCCCAGATAGAAGCCCG |
NGG |
2 |
0.2773 |
Tier II |
| 5 |
NC_000076.6 |
10 |
94395856 |
- |
Tmcc3 |
NNNTCCCAGAAATAAGCTCG |
NGG |
2 |
0.225 |
Tier II |
| 6 |
NC_000073.6 |
7 |
25237285 |
- |
Gsk3a |
NNNACCCAGAGAGAAGCTCG |
NGG |
2 |
0.1905 |
Tier II |
| 7 |
NC_000082.6 |
16 |
88242711 |
+ |
Grik1 |
NNNTCCCAGATAGAAACTCG |
NTG |
2 |
0.039 |
Tier II |
| 8 |
NC_000080.6 |
14 |
27130628 |
- |
Arhgef3 |
NNNTCCCAGATAGAAGCTCT |
NTG |
2 |
0.0273 |
Tier II |
| 9 |
NC_000068.7 |
2 |
135941534 |
+ |
Plcb4 |
NNNTCCTAGATAGAAGCTCG |
NGC |
2 |
0.0181 |
Tier II |
| 10 |
NC_000078.6 |
12 |
104475906 |
+ |
Gm10000 |
NNNTCCCAGAAAGAAGCTCC |
NGG |
2 |
0.3214 |
Tier III |
| 11 |
NC_000075.6 |
9 |
97014941 |
- |
Gm16010 |
NNNTCCCAGACAGAAGCTTG |
NGG |
2 |
0.3077 |
Tier III |
Other clones with same target sequence:
(none)
