Construct: sgRNA BRDN0001149112

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTATCCCAGATAGAAGCTCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C2B (5287)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76713

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 204469317 + PIK3C2B NNNTCCCAGATAGAAGCTCG NGG 0 1.0 Tier I
2 NC_000002.12 2 54232319 + ACYP2 NNNTCCCAGAAAGAAGCTCT NGG 2 0.525 Tier II
3 NC_000014.9 14 50231149 + SOS2 NNNTCCCCGTTAGAAGCTCG NGG 2 0.3782 Tier II
4 NC_000019.10 19 54596127 + LILRA1 NNNTCCCAGATAGAAGCCTG NGG 2 0.1978 Tier II
5 NC_000015.10 15 67125362 - SMAD3 NNNTCCCAGAGAGAAGATCG NGG 2 0.1867 Tier II
6 NC_000020.11 20 41518528 + CHD6 NNNTCCCAGACAGAAGCTGG NGG 2 0.0833 Tier II
7 NC_000023.11 X 150562938 + MTM1 NNNTCCCAGAGAGAAGCTGG NGG 2 0.05 Tier II
8 NC_000011.10 11 19275864 - CSRP3-AS1 NNNTCCCAGGAAGAAGCTCG NGG 2 0.4167 Tier III
9 NC_000004.12 4 104592637 + CXXC4-AS1 NNNTCCCAAATAGAAGCTAG NGG 2 0.2755 Tier III
10 NC_000004.12 4 104592637 + LOC124900745 NNNTCCCAAATAGAAGCTAG NGG 2 0.2755 Tier III
11 NC_000016.10 16 2406548 - ABCA17P NNNTCACAGATAGAAGCTGG NGG 2 0.1161 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 111258979 - Ttc28 NNNTCCCAGATAGGAGCTCT NGG 2 0.3267 Tier II
2 NC_000084.6 18 77516779 - Rnf165 NNNTGCCATATAGAAGCTCG NGG 2 0.32 Tier II
3 NC_000075.6 9 97014941 - Spsb4 NNNTCCCAGACAGAAGCTTG NGG 2 0.3077 Tier II
4 NC_000070.6 4 134744406 + Ldlrap1 NNNCCCCAGATAGAAGCCCG NGG 2 0.2773 Tier II
5 NC_000076.6 10 94395856 - Tmcc3 NNNTCCCAGAAATAAGCTCG NGG 2 0.225 Tier II
6 NC_000073.6 7 25237285 - Gsk3a NNNACCCAGAGAGAAGCTCG NGG 2 0.1905 Tier II
7 NC_000082.6 16 88242711 + Grik1 NNNTCCCAGATAGAAACTCG NTG 2 0.039 Tier II
8 NC_000080.6 14 27130628 - Arhgef3 NNNTCCCAGATAGAAGCTCT NTG 2 0.0273 Tier II
9 NC_000068.7 2 135941534 + Plcb4 NNNTCCTAGATAGAAGCTCG NGC 2 0.0181 Tier II
10 NC_000078.6 12 104475906 + Gm10000 NNNTCCCAGAAAGAAGCTCC NGG 2 0.3214 Tier III
11 NC_000075.6 9 97014941 - Gm16010 NNNTCCCAGACAGAAGCTTG NGG 2 0.3077 Tier III
Download CSV

Other clones with same target sequence:

(none)

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