Construct: sgRNA BRDN0001149113
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGCTTGATGTGACCCATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAST1 (22983)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76121
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000019.10 |
19 |
12865063 |
- |
MAST1 |
NNNCTTGATGTGACCCATGG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000005.10 |
5 |
67133531 |
- |
MAST4 |
NNNCTTTATGTGCCCCATGG |
NGG |
2 |
0.1203 |
Tier I |
3 |
NC_000001.11 |
1 |
46027748 |
- |
MAST2 |
NNNCTTGATGTGCCCCATGG |
NTG |
2 |
0.0082 |
Tier I |
4 |
NC_000018.10 |
18 |
49448684 |
- |
DYM |
NNNTTTGGTGTGACCCATGG |
NGG |
2 |
0.5867 |
Tier II |
5 |
NC_000020.11 |
20 |
44403447 |
- |
HNF4A |
NNNCTTGAGATGACCCATGG |
NGG |
2 |
0.5778 |
Tier II |
6 |
NC_000005.10 |
5 |
68283940 |
- |
PIK3R1 |
NNNCTATATGTGACCCATGG |
NGG |
2 |
0.4952 |
Tier II |
7 |
NC_000007.14 |
7 |
45600287 |
+ |
ADCY1 |
NNNCATGAAGTGACCCATGG |
NGG |
2 |
0.4643 |
Tier II |
8 |
NC_000019.10 |
19 |
45180028 |
+ |
BLOC1S3 |
NNNCTTGGTGTCACCCATGG |
NGG |
2 |
0.3882 |
Tier II |
9 |
NC_000017.11 |
17 |
32285755 |
+ |
RHBDL3 |
NNNCTTCATGTCACCCATGG |
NGG |
2 |
0.364 |
Tier II |
10 |
NC_000002.12 |
2 |
85839714 |
- |
ST3GAL5 |
NNNCTTGGTGTGACCCACGG |
NGG |
2 |
0.3143 |
Tier II |
11 |
NC_000001.11 |
1 |
7687291 |
+ |
CAMTA1 |
NNNCAGGATGTGACCCATGG |
NGG |
2 |
0.2857 |
Tier II |
12 |
NC_000001.11 |
1 |
64857418 |
- |
JAK1 |
NNNCTTCATGGGACCCATGG |
NGG |
2 |
0.275 |
Tier II |
13 |
NC_000012.12 |
12 |
41011476 |
+ |
CNTN1 |
NNNTTTGATGTGACCCAGGG |
NGG |
2 |
0.2667 |
Tier II |
14 |
NC_000002.12 |
2 |
190502628 |
- |
NEMP2 |
NNNCTTCATGTGACCCATGG |
NAG |
2 |
0.1782 |
Tier II |
15 |
NC_000016.10 |
16 |
4577339 |
- |
C16orf96 |
NNNCTTGATGTTACCCAGGG |
NGG |
2 |
0.1282 |
Tier II |
16 |
NC_000011.10 |
11 |
122776067 |
+ |
UBASH3B |
NNNCTTGATATGACCCTTGG |
NGG |
2 |
0.1244 |
Tier II |
17 |
NC_000020.11 |
20 |
36898994 |
+ |
SAMHD1 |
NNNGTTGATGTGACTCATGG |
NGG |
2 |
0.0333 |
Tier II |
18 |
NC_000002.12 |
2 |
241899213 |
+ |
FAM240C |
NNNCTTGATGTGACCCCTGG |
NTG |
2 |
0.0069 |
Tier II |
19 |
NC_000020.11 |
20 |
17153470 |
+ |
LOC105372544 |
NNNCTCGCTGTGACCCATGG |
NGG |
2 |
0.3896 |
Tier III |
20 |
NC_000004.12 |
4 |
78672472 |
- |
LINC01094 |
NNNCTTGTTTTGACCCATGG |
NGG |
2 |
0.3482 |
Tier III |
21 |
NC_000002.12 |
2 |
6780711 |
+ |
LOC105373404 |
NNNCTTGAAGTGACACATGG |
NGG |
2 |
0.2063 |
Tier III |
22 |
NC_000012.12 |
12 |
89960733 |
- |
LOC105369890 |
NNNCTTGATGGGACCCATGC |
NGG |
2 |
0.1714 |
Tier III |
23 |
NC_000004.12 |
4 |
12594848 |
+ |
LOC105374492 |
NNNGTTGATGTGACCCATGG |
NGA |
2 |
0.0347 |
Tier III |
24 |
NC_000002.12 |
2 |
241899213 |
+ |
LINC01237 |
NNNCTTGATGTGACCCCTGG |
NTG |
2 |
0.0069 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000074.6 |
8 |
84921216 |
+ |
Mast1 |
NNNCTTGATGTGGCCCATGG |
NAG |
2 |
0.1691 |
Tier I |
2 |
NC_000071.6 |
5 |
125648938 |
+ |
Tmem132b |
NNNCCTAATGTGACCCATGG |
NGG |
2 |
1.0 |
Tier II |
3 |
NC_000077.6 |
11 |
32573757 |
- |
Stk10 |
NNNCTTGATGTAACCCATGA |
NGG |
2 |
0.875 |
Tier II |
4 |
NC_000085.6 |
19 |
17788309 |
- |
Pcsk5 |
NNNCTTTAAGTGACCCATGG |
NGG |
2 |
0.5306 |
Tier II |
5 |
NC_000067.6 |
1 |
135552393 |
+ |
Nav1 |
NNNCATGGTGTGACCCATGG |
NGG |
2 |
0.3667 |
Tier II |
6 |
NC_000086.7 |
X |
36927634 |
+ |
Septin6 |
NNNCATCATGTGACCCATGG |
NGG |
2 |
0.3438 |
Tier II |
7 |
NC_000069.6 |
3 |
152748699 |
+ |
Pigk |
NNNCTTGATGTGACCCAGGG |
NGG |
1 |
0.3333 |
Tier II |
8 |
NC_000068.7 |
2 |
49690938 |
+ |
Kif5c |
NNNCTTAATGTGTCCCATGG |
NGG |
2 |
0.3 |
Tier II |
9 |
NC_000076.6 |
10 |
60635406 |
+ |
Cdh23 |
NNNCTTGATGTGACAAATGG |
NGG |
2 |
0.2222 |
Tier II |
10 |
NC_000072.6 |
6 |
85954692 |
- |
Dusp11 |
NNNCTTGATGGTACCCATGG |
NGG |
2 |
0.1538 |
Tier II |
11 |
NC_000086.7 |
X |
59151019 |
+ |
Fgf13 |
NNNCTTGATGTGACCCATAG |
NGA |
2 |
0.0496 |
Tier II |
12 |
NC_000068.7 |
2 |
92780495 |
- |
Gm39880 |
NNNCTTGAAATGACCCATGG |
NGG |
2 |
0.8667 |
Tier III |
13 |
NC_000072.6 |
6 |
69916589 |
- |
Igk |
NNNCTTGATGTAGCCCATGG |
NGG |
2 |
0.6087 |
Tier III |
14 |
NC_000069.6 |
3 |
147141749 |
- |
Gm29771 |
NNNCTTGATGTGACCCACAG |
NGG |
2 |
0.3061 |
Tier III |
15 |
NC_000078.6 |
12 |
87640171 |
- |
Gm3742 |
NNNCTTGTTGTGGCCCATGG |
NGG |
2 |
0.2795 |
Tier III |
16 |
NC_000078.6 |
12 |
87837890 |
- |
Gm21038 |
NNNCTTGTTGTGGCCCATGG |
NGG |
2 |
0.2795 |
Tier III |
17 |
NC_000074.6 |
8 |
84921216 |
+ |
Gm38426 |
NNNCTTGATGTGGCCCATGG |
NAG |
2 |
0.1691 |
Tier III |
18 |
NC_000073.6 |
7 |
62684218 |
- |
Gm9445 |
NNNCTAGATGTGACCCATGG |
NGC |
2 |
0.0193 |
Tier III |
Other clones with same target sequence:
(none)