Construct: sgRNA BRDN0001149113

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGCTTGATGTGACCCATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAST1 (22983)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76121

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 12865063 - MAST1 NNNCTTGATGTGACCCATGG NGG 0 1.0 Tier I
2 NC_000005.10 5 67133531 - MAST4 NNNCTTTATGTGCCCCATGG NGG 2 0.1203 Tier I
3 NC_000001.11 1 46027748 - MAST2 NNNCTTGATGTGCCCCATGG NTG 2 0.0082 Tier I
4 NC_000018.10 18 49448684 - DYM NNNTTTGGTGTGACCCATGG NGG 2 0.5867 Tier II
5 NC_000020.11 20 44403447 - HNF4A NNNCTTGAGATGACCCATGG NGG 2 0.5778 Tier II
6 NC_000005.10 5 68283940 - PIK3R1 NNNCTATATGTGACCCATGG NGG 2 0.4952 Tier II
7 NC_000007.14 7 45600287 + ADCY1 NNNCATGAAGTGACCCATGG NGG 2 0.4643 Tier II
8 NC_000019.10 19 45180028 + BLOC1S3 NNNCTTGGTGTCACCCATGG NGG 2 0.3882 Tier II
9 NC_000017.11 17 32285755 + RHBDL3 NNNCTTCATGTCACCCATGG NGG 2 0.364 Tier II
10 NC_000002.12 2 85839714 - ST3GAL5 NNNCTTGGTGTGACCCACGG NGG 2 0.3143 Tier II
11 NC_000001.11 1 7687291 + CAMTA1 NNNCAGGATGTGACCCATGG NGG 2 0.2857 Tier II
12 NC_000001.11 1 64857418 - JAK1 NNNCTTCATGGGACCCATGG NGG 2 0.275 Tier II
13 NC_000012.12 12 41011476 + CNTN1 NNNTTTGATGTGACCCAGGG NGG 2 0.2667 Tier II
14 NC_000002.12 2 190502628 - NEMP2 NNNCTTCATGTGACCCATGG NAG 2 0.1782 Tier II
15 NC_000016.10 16 4577339 - C16orf96 NNNCTTGATGTTACCCAGGG NGG 2 0.1282 Tier II
16 NC_000011.10 11 122776067 + UBASH3B NNNCTTGATATGACCCTTGG NGG 2 0.1244 Tier II
17 NC_000020.11 20 36898994 + SAMHD1 NNNGTTGATGTGACTCATGG NGG 2 0.0333 Tier II
18 NC_000002.12 2 241899213 + FAM240C NNNCTTGATGTGACCCCTGG NTG 2 0.0069 Tier II
19 NC_000020.11 20 17153470 + LOC105372544 NNNCTCGCTGTGACCCATGG NGG 2 0.3896 Tier III
20 NC_000004.12 4 78672472 - LINC01094 NNNCTTGTTTTGACCCATGG NGG 2 0.3482 Tier III
21 NC_000002.12 2 6780711 + LOC105373404 NNNCTTGAAGTGACACATGG NGG 2 0.2063 Tier III
22 NC_000012.12 12 89960733 - LOC105369890 NNNCTTGATGGGACCCATGC NGG 2 0.1714 Tier III
23 NC_000004.12 4 12594848 + LOC105374492 NNNGTTGATGTGACCCATGG NGA 2 0.0347 Tier III
24 NC_000002.12 2 241899213 + LINC01237 NNNCTTGATGTGACCCCTGG NTG 2 0.0069 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 84921216 + Mast1 NNNCTTGATGTGGCCCATGG NAG 2 0.1691 Tier I
2 NC_000071.6 5 125648938 + Tmem132b NNNCCTAATGTGACCCATGG NGG 2 1.0 Tier II
3 NC_000077.6 11 32573757 - Stk10 NNNCTTGATGTAACCCATGA NGG 2 0.875 Tier II
4 NC_000085.6 19 17788309 - Pcsk5 NNNCTTTAAGTGACCCATGG NGG 2 0.5306 Tier II
5 NC_000067.6 1 135552393 + Nav1 NNNCATGGTGTGACCCATGG NGG 2 0.3667 Tier II
6 NC_000086.7 X 36927634 + Septin6 NNNCATCATGTGACCCATGG NGG 2 0.3438 Tier II
7 NC_000069.6 3 152748699 + Pigk NNNCTTGATGTGACCCAGGG NGG 1 0.3333 Tier II
8 NC_000068.7 2 49690938 + Kif5c NNNCTTAATGTGTCCCATGG NGG 2 0.3 Tier II
9 NC_000076.6 10 60635406 + Cdh23 NNNCTTGATGTGACAAATGG NGG 2 0.2222 Tier II
10 NC_000072.6 6 85954692 - Dusp11 NNNCTTGATGGTACCCATGG NGG 2 0.1538 Tier II
11 NC_000086.7 X 59151019 + Fgf13 NNNCTTGATGTGACCCATAG NGA 2 0.0496 Tier II
12 NC_000068.7 2 92780495 - Gm39880 NNNCTTGAAATGACCCATGG NGG 2 0.8667 Tier III
13 NC_000072.6 6 69916589 - Igk NNNCTTGATGTAGCCCATGG NGG 2 0.6087 Tier III
14 NC_000069.6 3 147141749 - Gm29771 NNNCTTGATGTGACCCACAG NGG 2 0.3061 Tier III
15 NC_000078.6 12 87640171 - Gm3742 NNNCTTGTTGTGGCCCATGG NGG 2 0.2795 Tier III
16 NC_000078.6 12 87837890 - Gm21038 NNNCTTGTTGTGGCCCATGG NGG 2 0.2795 Tier III
17 NC_000074.6 8 84921216 + Gm38426 NNNCTTGATGTGGCCCATGG NAG 2 0.1691 Tier III
18 NC_000073.6 7 62684218 - Gm9445 NNNCTAGATGTGACCCATGG NGC 2 0.0193 Tier III
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Other clones with same target sequence:

(none)