Construct: sgRNA BRDN0001149114
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGCAAAATCATCACTACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AAK1 (22848)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77938
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
69530642 |
- |
AAK1 |
NNNCAAAATCATCACTACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
238178490 |
- |
ILKAP |
NNNCAAAATTATCACTACTA |
NGG |
2 |
0.6275 |
Tier II |
| 3 |
NC_000002.12 |
2 |
36802059 |
- |
VIT |
NNNCACAATCATCACTACAA |
NGG |
2 |
0.3247 |
Tier II |
| 4 |
NC_000002.12 |
2 |
237512916 |
+ |
MLPH |
NNNCAAAATCATCACCACCA |
NGG |
2 |
0.2989 |
Tier II |
| 5 |
NC_000003.12 |
3 |
183093937 |
- |
MCCC1 |
NNNGAAAATCATAACTACGA |
NGG |
2 |
0.1923 |
Tier II |
| 6 |
NC_000016.10 |
16 |
20993905 |
- |
DNAH3 |
NNNCAAAATGATCACTACCA |
NGG |
2 |
0.1743 |
Tier II |
| 7 |
NC_000008.11 |
8 |
139617278 |
+ |
KCNK9 |
NNNCAAAATCATCAATAAGA |
NGG |
2 |
0.1197 |
Tier II |
| 8 |
NC_000001.11 |
1 |
10733098 |
+ |
CASZ1 |
NNNCAAAATCATCACTGAGA |
NGG |
2 |
0.095 |
Tier II |
| 9 |
NC_000002.12 |
2 |
36802059 |
- |
LOC124905990 |
NNNCACAATCATCACTACAA |
NGG |
2 |
0.3247 |
Tier III |
| 10 |
NC_000002.12 |
2 |
76582843 |
+ |
LOC105374814 |
NNNCAAAATCATCACTACCA |
NGA |
2 |
0.0311 |
Tier III |
| 11 |
NC_000011.10 |
11 |
30845054 |
+ |
LOC107984419 |
NNNCTAAATCATCACTACGA |
NGT |
2 |
0.0059 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
86946169 |
+ |
Aak1 |
NNNCAAAATCATCACTACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
103540874 |
+ |
Gm884 |
NNNCAAAACTATCACTACGA |
NGG |
2 |
0.8688 |
Tier I |
| 3 |
NC_000072.6 |
6 |
92648944 |
+ |
Prickle2 |
NNNCAAAATCAGCACCACGA |
NGG |
2 |
0.3333 |
Tier II |
| 4 |
NC_000081.6 |
15 |
78432560 |
+ |
Kctd17 |
NNNCAAACTCATCACTACCA |
NGG |
2 |
0.1921 |
Tier II |
| 5 |
NC_000073.6 |
7 |
92363004 |
+ |
Dlg2 |
NNNCAAAATCATCACTGCCA |
NGG |
2 |
0.0791 |
Tier II |
| 6 |
NC_000079.6 |
13 |
104296726 |
- |
Adamts6 |
NNNCAAAATCATCACTACGA |
NTG |
1 |
0.039 |
Tier II |
| 7 |
NC_000080.6 |
14 |
86886248 |
+ |
Diaph3 |
NNNCAAAATCATCAGTACGG |
NGG |
2 |
0.0382 |
Tier II |
| 8 |
NC_000071.6 |
5 |
9373162 |
- |
Elapor2 |
NNNCAAAATCATCACTAAGA |
NGT |
2 |
0.0087 |
Tier II |
| 9 |
NC_000081.6 |
15 |
11363347 |
- |
Gm34444 |
NNNCAAAATCATCAATACGA |
NGC |
2 |
0.0049 |
Tier III |
Other clones with same target sequence:
(none)
