Construct: sgRNA BRDN0001149120
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGAAGTTACTTGAACGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK33 (65975)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76986
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
8461879 |
- |
STK33 |
NNNGAAGTTACTTGAACGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
75337919 |
- |
CFDP1 |
NNNGAAGTCACTTGAAAGAG |
NGG |
2 |
0.4308 |
Tier II |
| 3 |
NC_000001.11 |
1 |
231885582 |
- |
DISC1 |
NNNGAAGGTACTTGAAAGAG |
NGG |
2 |
0.3422 |
Tier II |
| 4 |
NC_000019.10 |
19 |
47572533 |
+ |
ZNF541 |
NNNGAAGTTACTTAAAGGAG |
NGG |
2 |
0.0441 |
Tier II |
| 5 |
NC_000014.9 |
14 |
28872392 |
+ |
LINC02327 |
NNNGAAGTTACTTGAACTAA |
NGG |
2 |
0.625 |
Tier III |
| 6 |
NC_000014.9 |
14 |
97578397 |
+ |
LINC02325 |
NNNGAAGATACTTGAACTAG |
NGG |
2 |
0.5333 |
Tier III |
| 7 |
NC_000001.11 |
1 |
231885582 |
- |
TSNAX-DISC1 |
NNNGAAGGTACTTGAAAGAG |
NGG |
2 |
0.3422 |
Tier III |
| 8 |
NC_000006.12 |
6 |
74360940 |
- |
LOC101928516 |
NNNGAAGTTCCTTGAAAGAG |
NGG |
2 |
0.1556 |
Tier III |
| 9 |
NC_000007.14 |
7 |
124931936 |
- |
POT1-AS1 |
NNNGAAGTTACTTGAACGTG |
NAG |
2 |
0.1396 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
14602659 |
- |
Dlgap2 |
NNNGAAATTACTTGAAAGAG |
NGG |
2 |
0.4667 |
Tier II |
| 2 |
NC_000074.6 |
8 |
49084260 |
+ |
Tenm3 |
NNNGAAGTTACATGAACGTG |
NGG |
2 |
0.4308 |
Tier II |
| 3 |
NC_000072.6 |
6 |
125041240 |
+ |
Ing4 |
NNNTAAGTTACTTGAACGAG |
NAG |
2 |
0.0943 |
Tier II |
| 4 |
NC_000072.6 |
6 |
125041240 |
+ |
4930557K07Rik |
NNNTAAGTTACTTGAACGAG |
NAG |
2 |
0.0943 |
Tier III |
| 5 |
NC_000072.6 |
6 |
67921107 |
- |
Igk |
NNNGAAGTTATTTGAACGAG |
NAG |
2 |
0.0798 |
Tier III |
Other clones with same target sequence:
(none)
