Construct: sgRNA BRDN0001149130

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTTCTTGCTCCAATCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BLK (640)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75751

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 11550218 + BLK NNNTCTTGCTCCAATCAACA NGG 0 1.0 Tier I
2 NC_000013.11 13 19912708 - ZMYM2 NNNTCTAGTTCCAATCAACA NGG 2 0.7656 Tier II
3 NC_000012.12 12 125274327 + TMEM132B NNNCTTTGCTCCAATCAACA NGG 2 0.4118 Tier II
4 NC_000011.10 11 126872077 - KIRREL3 NNNTCCTGCTCCAATCAGCA NGG 2 0.3636 Tier II
5 NC_000003.12 3 47961939 - MAP4 NNNTCTTGTTCCAATCAGCA NGG 2 0.35 Tier II
6 NC_000020.11 20 19330069 - SLC24A3 NNNTCTTGCTGCAATAAACA NGG 2 0.25 Tier II
7 NC_000001.11 1 76106353 - ST6GALNAC3 NNNTCTTGCACCAACCAACA NGG 2 0.2338 Tier II
8 NC_000002.12 2 120913705 - GLI2 NNNTCTTTCTGCAATCAACA NGG 2 0.1563 Tier II
9 NC_000011.10 11 46030324 + PHF21A NNNTCTTGCTCCAATCAAGA NTG 2 0.0049 Tier II
10 NC_000015.10 15 82497472 - GOLGA2P10 NNNTCTTGATCAAATCAACA NGG 2 0.6122 Tier III
11 NC_000015.10 15 84224656 - GOLGA2P7 NNNTCTTGATCAAATCAACA NGG 2 0.6122 Tier III
12 NC_000015.10 15 84448929 + LOC102724135 NNNTCTTGATCAAATCAACA NGG 2 0.6122 Tier III
13 NC_000015.10 15 85229283 - LOC727751 NNNTCTTGATCAAATCAACA NGG 2 0.6122 Tier III
14 NC_000015.10 15 85229283 - LOC101929479 NNNTCTTGATCAAATCAACA NGG 2 0.6122 Tier III
15 NC_000012.12 12 125274327 + LOC105370054 NNNCTTTGCTCCAATCAACA NGG 2 0.4118 Tier III
16 NC_000011.10 11 126872077 - LOC105369559 NNNTCCTGCTCCAATCAGCA NGG 2 0.3636 Tier III
17 NC_000004.12 4 152292788 + LOC105377492 NNNTCTTGGCCCAATCAACA NGG 2 0.3302 Tier III
18 NC_000015.10 15 34765457 - GJD2-DT NNNTCTTGCTCCAAACAACC NGG 2 0.1316 Tier III
19 NC_000003.12 3 143127829 - LOC105374137 NNNTCTTGCTGCAATCATCA NGG 2 0.125 Tier III
20 NC_000022.11 22 25382963 + LRP5L NNNTCTTGCTCCAACCAGCA NGG 2 0.1091 Tier III
21 NC_000008.11 8 59615594 - LOC105375859 NNNTCTTGCTCCTATCAACA NTG 2 0.0117 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 30658777 + Ctnnd2 NNNTCTTAATCCAATCAACA NGG 2 0.8571 Tier II
2 NC_000074.6 8 122431578 - Cyba NNNTCTAGGTCCAATCAACA NGG 2 0.5417 Tier II
3 NC_000077.6 11 64024199 - Cox10 NNNTCTTGCTCCATTCAACA NGG 1 0.5333 Tier II
4 NC_000082.6 16 45179500 - Atg3 NNNTGTTCCTCCAATCAACA NGG 2 0.3692 Tier II
5 NC_000074.6 8 28975796 + Unc5d NNNTCTAGCTCCAATCAGCA NGG 2 0.35 Tier II
6 NC_000078.6 12 51755256 - Hectd1 NNNTCTTACTCCAACCAACA NGG 2 0.2727 Tier II
7 NC_000077.6 11 22072853 + Ehbp1 NNNTCTTGCTCCAATAAACA NAG 2 0.2593 Tier II
8 NC_000078.6 12 110478896 + Ppp2r5c NNNACTTGCTCGAATCAACA NGG 2 0.2116 Tier II
9 NC_000083.6 17 35963080 + Abcf1 NNNTCTTGCTCCAGTCAGCA NGG 2 0.1867 Tier II
10 NC_000071.6 5 27593434 + Dpp6 NNNTCTTGCTTCAATCAAAA NGG 2 0.1319 Tier II
11 NC_000073.6 7 92125788 - Dlg2 NNNTCTTTCTCCAATCAACA NTG 2 0.0244 Tier II
12 NC_000084.6 18 65730993 + Oacyl NNNTGTTGCTCCAATCAACA NGT 2 0.0097 Tier II
13 NC_000070.6 4 10702966 + 1700123O12Rik NNNTTTTGCTCCAATCAACA NGG 1 0.6364 Tier III
14 NC_000070.6 4 128922070 + Gm33901 NNNTCTTTCTCCAAACAACA NGG 2 0.3618 Tier III
15 NC_000073.6 7 100169116 + Gm39057 NNNTCTTGCTCCATTCAAAA NGG 2 0.2286 Tier III
16 NC_000078.6 12 110478896 + Gm19605 NNNACTTGCTCGAATCAACA NGG 2 0.2116 Tier III
17 NC_000078.6 12 73506989 + Gm34016 NNNTCTTGCTCCATTCAACA NAG 2 0.1383 Tier III
18 NC_000084.6 18 55424440 - Gm34233 NNNACTTGCTCCAATGAACA NGG 2 0.0733 Tier III
19 NC_000076.6 10 117414022 + Gm40770 NNNTCTTGCTCCAACCCACA NGG 2 0.0481 Tier III
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Other clones with same target sequence:

(none)