Construct: sgRNA BRDN0001149132
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGCCTCTACAAAAACTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3CG (5294)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76335
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
106868701 |
- |
PIK3CG |
NNNCCTCTACAAAAACTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
8152282 |
- |
PTPRM |
NNNACTCTACAAAAACTCTG |
NGG |
2 |
0.401 |
Tier II |
| 3 |
NC_000017.11 |
17 |
56264884 |
+ |
ANKFN1 |
NNNCCTCTACAAATACTTTG |
NGG |
2 |
0.3556 |
Tier II |
| 4 |
NC_000001.11 |
1 |
209757400 |
+ |
TRAF3IP3 |
NNNCCACTACAAAACCTGTG |
NGG |
2 |
0.2364 |
Tier II |
| 5 |
NC_000006.12 |
6 |
46240181 |
+ |
RCAN2 |
NNNCCTCCACAACAACTGTG |
NGG |
2 |
0.2105 |
Tier II |
| 6 |
NC_000006.12 |
6 |
132511978 |
+ |
STX7 |
NNNCCTCTACAGAAACTGTG |
NAG |
2 |
0.1872 |
Tier II |
| 7 |
NC_000009.12 |
9 |
124335784 |
+ |
NEK6 |
NNNCCTCTCCAAAAACTGCG |
NGG |
2 |
0.1576 |
Tier II |
| 8 |
NC_000004.12 |
4 |
182463353 |
+ |
TENM3 |
NNNCCACTACAAAAACTGTG |
NGA |
2 |
0.0602 |
Tier II |
| 9 |
NC_000005.10 |
5 |
38724361 |
+ |
OSMR-DT |
NNNCCTTTACAAAAAATGTG |
NGG |
2 |
0.8125 |
Tier III |
| 10 |
NC_000004.12 |
4 |
105341364 |
- |
TET2-AS1 |
NNNGATCTACAAAAACTGTG |
NGG |
2 |
0.2857 |
Tier III |
| 11 |
NC_000006.12 |
6 |
46240181 |
+ |
LOC105375079 |
NNNCCTCCACAACAACTGTG |
NGG |
2 |
0.2105 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
53223233 |
- |
Ddx10 |
NNNCCTCTACAGAAACTCTG |
NGG |
2 |
0.3439 |
Tier II |
| 2 |
NC_000074.6 |
8 |
10439191 |
- |
Myo16 |
NNNCCTCTCCAAATACTGTG |
NGG |
2 |
0.3048 |
Tier II |
| 3 |
NC_000070.6 |
4 |
111647376 |
- |
Agbl4 |
NNNCTTCTAGAAAAACTGTG |
NGG |
2 |
0.2475 |
Tier II |
| 4 |
NC_000072.6 |
6 |
138038291 |
+ |
Slc15a5 |
NNNCCTCTACAGAACCTGTG |
NGG |
2 |
0.197 |
Tier II |
| 5 |
NC_000069.6 |
3 |
28005800 |
- |
Pld1 |
NNNCCTCTCCAACAACTGTG |
NGG |
2 |
0.1203 |
Tier II |
| 6 |
NC_000075.6 |
9 |
78137401 |
- |
Cilk1 |
NNNCCTCTACAAAAACAGTG |
NGT |
2 |
0.0086 |
Tier II |
| 7 |
NC_000078.6 |
12 |
77295081 |
+ |
Fut8 |
NNNCCTCTACAAAAACAGTG |
NGT |
2 |
0.0086 |
Tier II |
| 8 |
NC_000067.6 |
1 |
21244188 |
- |
Gsta3 |
NNNCCTCTACAAATACTGTG |
NGT |
2 |
0.0086 |
Tier II |
| 9 |
NC_000083.6 |
17 |
29072690 |
+ |
Trp53cor1 |
NNNCCTTTACAAAACCTGTG |
NGG |
2 |
0.2216 |
Tier III |
| 10 |
NC_000067.6 |
1 |
21244188 |
- |
Gm28836 |
NNNCCTCTACAAATACTGTG |
NGT |
2 |
0.0086 |
Tier III |
Other clones with same target sequence:
(none)
