Construct: sgRNA BRDN0001149133

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTTGTGAAGGATCCTAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PI4K2B (55300)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77133

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 25252471 + PI4K2B NNNTGTGAAGGATCCTAAGA NGG 0 1.0 Tier I
2 NC_000006.12 6 38686932 - GLO1 NNNAGTGAAGGATCCTAAGA NGT 2 0.0077 Tier I
3 NC_000022.11 22 21802114 + MAPK1 NNNTCAGAAGGATCCTAAGA NGG 2 0.681 Tier II
4 NC_000006.12 6 47803244 + OPN5 NNNTGTGAAGGAACCAAAGA NGG 2 0.6294 Tier II
5 NC_000020.11 20 38575912 + RALGAPB NNNTGTGAAGGATTCTAATA NGG 2 0.4889 Tier II
6 NC_000022.11 22 21438065 + HIC2 NNNTGAGAAGGATCCTAGGA NGG 2 0.3467 Tier II
7 NC_000003.12 3 111932593 - PHLDB2 NNNTGTGAAGGATTCTAACA NGG 2 0.3287 Tier II
8 NC_000014.9 14 33366633 - NPAS3 NNNTGTTAAGCATCCTAAGA NGG 2 0.2449 Tier II
9 NC_000010.11 10 97852079 + GOLGA7B NNNTGTGAAGGAGCCAAAGA NGG 2 0.2372 Tier II
10 NC_000003.12 3 158706199 + RARRES1 NNNTGTGAAGGATACCAAGA NGG 2 0.2333 Tier II
11 NC_000012.12 12 51487752 - SLC4A8 NNNTGAGAAGGAGCCTAAGA NGG 2 0.2261 Tier II
12 NC_000017.11 17 57569881 + MSI2 NNNAGTGCAGGATCCTAAGA NGG 2 0.2041 Tier II
13 NC_000023.11 X 148992734 - AFF2 NNNTGTGAAGGATCATAAGG NGG 2 0.1699 Tier II
14 NC_000016.10 16 70781779 + VAC14 NNNTGTGATGGATCCTAAGA NAG 2 0.1556 Tier II
15 NC_000010.11 10 5012397 + AKR1C2 NNNTGTTAAGGATCCTAAGA NAG 2 0.1481 Tier II
16 NC_000002.12 2 112585542 - CHCHD5 NNNTCTGAAGGATCCTGAGA NGG 2 0.1387 Tier II
17 NC_000008.11 8 25883927 - EBF2 NNNTGGGAAGGATCCTGAGA NGG 2 0.1008 Tier II
18 NC_000005.10 5 38453917 - EGFLAM NNNTTTGAAGGATCCTAAGC NGG 2 0.0682 Tier II
19 NC_000007.14 7 66930412 + TMEM248 NNNTTTGAAGGATCCTGAGA NGG 2 0.0529 Tier II
20 NC_000012.12 12 110690297 - HVCN1 NNNTGTGAAGGATCCTGAGC NGG 2 0.0401 Tier II
21 NC_000011.10 11 1109015 - MUC2 NNNAGTGAAGGATCCTAAGA NGA 2 0.0331 Tier II
22 NC_000002.12 2 109670168 - RANBP2 NNNTGTGAAGGATGCTAAGG NGG 2 0.0 Tier II
23 NC_000003.12 3 33013306 - GLB1 NNNTGTGATGGATGCTAAGA NGG 2 0.0 Tier II
24 NC_000019.10 19 19483503 + GATAD2A NNNTGTGATGGATGCTAAGA NGG 2 0.0 Tier II
25 NC_000009.12 9 2900807 - ATP5PDP2 NNNGGTGAAGGATCCCAAGA NGG 2 0.4167 Tier III
26 NC_000009.12 9 14068930 + ATP5PDP3 NNNGGTGAAGGATCCCAAGA NGG 2 0.4167 Tier III
27 NC_000011.10 11 102823483 - WTAPP1 NNNAGTGGAGGATCCTAAGA NGG 2 0.3492 Tier III
28 NC_000022.11 22 18557276 - LOC124905077 NNNTGAGAAGGATCCTAGGA NGG 2 0.3467 Tier III
29 NC_000022.11 22 21496801 - PI4KAP2 NNNTGAGAAGGATCCTAGGA NGG 2 0.3467 Tier III
30 NC_000017.11 17 57569881 + LOC107984993 NNNAGTGCAGGATCCTAAGA NGG 2 0.2041 Tier III
31 NC_000003.12 3 4852506 + LOC124906209 NNNTGAGAAGGATCCTGAGA NGG 2 0.1529 Tier III
32 NC_000002.12 2 30861086 - LOC124905982 NNNTGTGAAGGATCCTCAGG NGG 2 0.1349 Tier III
33 NC_000011.10 11 112535656 - LINC02764 NNNTGTCAAGGATCCTCAGA NGG 2 0.1213 Tier III
34 NC_000011.10 11 112535656 - LINC02763 NNNTGTCAAGGATCCTCAGA NGG 2 0.1213 Tier III
35 NC_000002.12 2 3020374 + LINC01250 NNNAGTGAAGGATCCTGAGA NGG 2 0.084 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 33142397 - Wdfy4 NNNTGTGCAGGATCCTGAGA NGG 2 0.0756 Tier I
2 NC_000068.7 2 69095929 + Cers6 NNNTGAGAAGGATTCTAAGA NGG 2 0.6356 Tier II
3 NC_000081.6 15 94807560 - Tmem117 NNNTCTGAGGGATCCTAAGA NGG 2 0.5238 Tier II
4 NC_000067.6 1 190825985 - Rps6kc1 NNNTGTGAAAGATCCTATGA NGG 2 0.4667 Tier II
5 NC_000082.6 16 26664570 - Il1rap NNNTGAGAAGTATCCTAAGA NGG 2 0.3333 Tier II
6 NC_000071.6 5 102760688 + Arhgap24 NNNTTTGAAGGATCCAAAGA NGG 2 0.2727 Tier II
7 NC_000074.6 8 47206266 - Stox2 NNNTTTGAAGGATCCAAAGA NGG 2 0.2727 Tier II
8 NC_000067.6 1 135546873 - Nav1 NNNTGTGAAGGATCCTGAGT NGG 2 0.1059 Tier II
9 NC_000080.6 14 93634431 + Pcdh9 NNNTGTGAAGTCTCCTAAGA NGG 2 0.1012 Tier II
10 NC_000080.6 14 115104415 - Gpc5 NNNTGTGAAGGATACTGAGA NGG 2 0.0618 Tier II
11 NC_000075.6 9 66036957 - Csnk1g1 NNNTGTGGAGGATCCTAAGA NGA 2 0.0509 Tier II
12 NC_000071.6 5 71646617 - Gabra4 NNNTGTGAAGGATCCCAAGA NGA 2 0.0463 Tier II
13 NC_000082.6 16 42150242 + Lsamp NNNTGTGAAGGATCTGAAGA NGG 2 0.0231 Tier II
14 NC_000074.6 8 115323676 - Wwox NNNTGGGAAGGATCCTAAGA NGC 2 0.0127 Tier II
15 NC_000070.6 4 98465897 + Patj NNNTGTGAAGGATGCTATGA NGG 2 0.0 Tier II
16 NC_000079.6 13 10450524 + Gm26861 NNNTGTGAAGGATCCTAAGA NGG 0 1.0 Tier III
17 NC_000067.6 1 190825985 - Gm51740 NNNTGTGAAAGATCCTATGA NGG 2 0.4667 Tier III
18 NC_000073.6 7 100203047 + Gm34877 NNNTGTGAAGGATCCTAGGA NGA 2 0.0278 Tier III
Download CSV

Other clones with same target sequence:

(none)