Construct: sgRNA BRDN0001149136
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGGTGGAACGTGTTGTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CKMT1A (548596)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75879
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
43595983 |
+ |
CKMT1B |
NNNGGTGGAACGTGTTGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
43695815 |
+ |
CKMT1A |
NNNGGTGGAACGTGTTGTGG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000003.12 |
3 |
98894244 |
- |
DCBLD2 |
NNNGCTGGAACGTATTGTGG |
NGG |
2 |
0.5893 |
Tier II |
| 4 |
NC_000018.10 |
18 |
4448914 |
- |
DLGAP1 |
NNNGGTGGAAAGTGTTGTGG |
NAG |
2 |
0.1944 |
Tier II |
| 5 |
NC_000020.11 |
20 |
51800245 |
- |
SALL4 |
NNNGGTGGGACGGGTTGTGG |
NGG |
2 |
0.1739 |
Tier II |
| 6 |
NC_000008.11 |
8 |
36795553 |
+ |
KCNU1 |
NNNGGTGGAAGGTGATGTGG |
NGG |
2 |
0.1447 |
Tier II |
| 7 |
NC_000017.11 |
17 |
5110545 |
+ |
ZNF232 |
NNNGGTGGAATGTGTTGGGG |
NGG |
2 |
0.1026 |
Tier II |
| 8 |
NC_000008.11 |
8 |
902972 |
+ |
DLGAP2 |
NNNGGTGGAACGTGTTCGGG |
NGG |
2 |
0.0784 |
Tier II |
| 9 |
NC_000003.12 |
3 |
106392190 |
+ |
LOC101929485 |
NNNAGTGGAATGTGTTGTGG |
NGG |
2 |
0.2769 |
Tier III |
| 10 |
NC_000008.11 |
8 |
36795553 |
+ |
MRPS7P1 |
NNNGGTGGAAGGTGATGTGG |
NGG |
2 |
0.1447 |
Tier III |
| 11 |
NC_000001.11 |
1 |
94683703 |
+ |
SLC44A3-AS1 |
NNNGGTGGAATGTGCTGTGG |
NGG |
2 |
0.0839 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
121360683 |
+ |
Ckmt1 |
NNNGGTAGAACGTGTTGTGG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000079.6 |
13 |
14187921 |
+ |
Arid4b |
NNNGGTGGACAGTGTTGTGG |
NGG |
2 |
0.25 |
Tier I |
| 3 |
NC_000069.6 |
3 |
108145559 |
- |
Gnai3 |
NNNGCTGGAACGTGTTGAGG |
NGG |
2 |
0.5238 |
Tier II |
| 4 |
NC_000067.6 |
1 |
91251688 |
+ |
Ube2f |
NNNGGTGGGACGTGTTGAGG |
NGG |
2 |
0.4444 |
Tier II |
| 5 |
NC_000073.6 |
7 |
125422493 |
- |
Gm30717 |
NNNGGTGGAATGTGTGGTGG |
NGG |
2 |
0.1065 |
Tier III |
| 6 |
NC_000073.6 |
7 |
35130806 |
- |
Gm35724 |
NNNGGTGGGACGTGTTGTGG |
NTG |
2 |
0.026 |
Tier III |
| 7 |
NC_000073.6 |
7 |
35130806 |
- |
Gm51475 |
NNNGGTGGGACGTGTTGTGG |
NTG |
2 |
0.026 |
Tier III |
Other clones with same target sequence:
(none)
