Construct: sgRNA BRDN0001149147

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGCACCGCTAAGAAGAATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RIPK1 (8737)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76535

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 3083183 + RIPK1 NNNACCGCTAAGAAGAATGG NGG 0 1.0 Tier I
2 NC_000004.12 4 5975480 - C4orf50 NNNACCGCAAAGAAGAATGA NGG 2 0.8705 Tier II
3 NC_000011.10 11 133014024 + OPCML NNNACCGCTAGGAAGAATGA NGG 2 0.6094 Tier II
4 NC_000004.12 4 94852228 - BMPR1B NNNACATCTAAGAAGAATGG NGG 2 0.5306 Tier II
5 NC_000012.12 12 304577 - KDM5A NNNACAGCTGAGAAGAATGG NGG 2 0.5159 Tier II
6 NC_000009.12 9 101705363 + GRIN3A NNNACCGCCAAGAAGAACGG NGG 2 0.3956 Tier II
7 NC_000010.11 10 71489239 - CDH23 NNNAAGGCTAAGAAGAATGG NGG 2 0.2857 Tier II
8 NC_000007.14 7 137917626 + CREB3L2 NNNACCGCTAAGAAGAAGGG NAG 2 0.0864 Tier II
9 NC_000003.12 3 37722444 - ITGA9 NNNACAGCTAAGAAGTATGG NGG 2 0.0 Tier II
10 NC_000003.12 3 140188415 + CLSTN2 NNNACCACTAAGAAGCATGG NGG 2 0.0 Tier II
11 NC_000007.14 7 106811495 - LINC02577 NNNACCACAAAGAAGAATGG NGG 2 0.9286 Tier III
12 NC_000003.12 3 55229046 - LINC02030 NNNACTGCAAAGAAGAATGG NGG 2 0.8622 Tier III
13 NC_000003.12 3 55229046 - LOC124906243 NNNACTGCAAAGAAGAATGG NGG 2 0.8622 Tier III
14 NC_000010.11 10 90465223 - LINC02653 NNNACAGCTAAGGAGAATGG NGG 2 0.6056 Tier III
15 NC_000011.10 11 27155270 - BBOX1-AS1 NNNAGCCCTAAGAAGAATGG NGG 2 0.4125 Tier III
16 NC_000010.11 10 63987735 + LOC124902439 NNNACCCCTAAGATGAATGG NGG 2 0.3667 Tier III
17 NC_000003.12 3 61342114 - LOC105377114 NNNACCGCTAAGAAAAATGG NCG 2 0.1008 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 103568092 + Chl1 NNNACCACTAAGAAAAATGG NGG 2 0.9412 Tier II
2 NC_000067.6 1 164134074 - Selp NNNACTGCTAAGAAAAATGG NGG 2 0.8739 Tier II
3 NC_000069.6 3 89582552 - Kcnn3 NNNACAGCAAAGAAGAATGG NGG 2 0.8622 Tier II
4 NC_000079.6 13 41016775 + Tmem14c NNNGACGCTAAGAAGAATGG NGG 2 0.3571 Tier II
5 NC_000075.6 9 34791274 - Kirrel3 NNNATGGCTAAGAAGAATGG NGG 2 0.3182 Tier II
6 NC_000068.7 2 18210895 + Dnajc1 NNNACAGCTATGAAGAATGG NGG 2 0.2857 Tier II
7 NC_000068.7 2 18210895 + Mllt10 NNNACAGCTATGAAGAATGG NGG 2 0.2857 Tier II
8 NC_000076.6 10 110346154 - Nav3 NNNACCCCTAAGAAGAAGGG NGG 2 0.2292 Tier II
9 NC_000074.6 8 115183899 - Wwox NNNACTGCTAAGAAGAATGG NTG 2 0.0362 Tier II
10 NC_000070.6 4 86521616 + Saxo1 NNNACGGCTAAGAAGAATGG NGC 2 0.0111 Tier II
11 NC_000072.6 6 94057296 + Magi1 NNNACCGCTATGAAGAATGG NGT 2 0.005 Tier II
12 NC_000067.6 1 90443548 - Gm34992 NNNCCAGCTAAGAAGAATGG NGG 2 0.3277 Tier III
Download CSV

Other clones with same target sequence:

(none)

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