Construct: sgRNA BRDN0001149147
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCACCGCTAAGAAGAATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RIPK1 (8737)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76535
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
3083183 |
+ |
RIPK1 |
NNNACCGCTAAGAAGAATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
5975480 |
- |
C4orf50 |
NNNACCGCAAAGAAGAATGA |
NGG |
2 |
0.8705 |
Tier II |
| 3 |
NC_000011.10 |
11 |
133014024 |
+ |
OPCML |
NNNACCGCTAGGAAGAATGA |
NGG |
2 |
0.6094 |
Tier II |
| 4 |
NC_000004.12 |
4 |
94852228 |
- |
BMPR1B |
NNNACATCTAAGAAGAATGG |
NGG |
2 |
0.5306 |
Tier II |
| 5 |
NC_000012.12 |
12 |
304577 |
- |
KDM5A |
NNNACAGCTGAGAAGAATGG |
NGG |
2 |
0.5159 |
Tier II |
| 6 |
NC_000009.12 |
9 |
101705363 |
+ |
GRIN3A |
NNNACCGCCAAGAAGAACGG |
NGG |
2 |
0.3956 |
Tier II |
| 7 |
NC_000010.11 |
10 |
71489239 |
- |
CDH23 |
NNNAAGGCTAAGAAGAATGG |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000007.14 |
7 |
137917626 |
+ |
CREB3L2 |
NNNACCGCTAAGAAGAAGGG |
NAG |
2 |
0.0864 |
Tier II |
| 9 |
NC_000003.12 |
3 |
37722444 |
- |
ITGA9 |
NNNACAGCTAAGAAGTATGG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000003.12 |
3 |
140188415 |
+ |
CLSTN2 |
NNNACCACTAAGAAGCATGG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000007.14 |
7 |
106811495 |
- |
LINC02577 |
NNNACCACAAAGAAGAATGG |
NGG |
2 |
0.9286 |
Tier III |
| 12 |
NC_000003.12 |
3 |
55229046 |
- |
LINC02030 |
NNNACTGCAAAGAAGAATGG |
NGG |
2 |
0.8622 |
Tier III |
| 13 |
NC_000003.12 |
3 |
55229046 |
- |
LOC124906243 |
NNNACTGCAAAGAAGAATGG |
NGG |
2 |
0.8622 |
Tier III |
| 14 |
NC_000010.11 |
10 |
90465223 |
- |
LINC02653 |
NNNACAGCTAAGGAGAATGG |
NGG |
2 |
0.6056 |
Tier III |
| 15 |
NC_000011.10 |
11 |
27155270 |
- |
BBOX1-AS1 |
NNNAGCCCTAAGAAGAATGG |
NGG |
2 |
0.4125 |
Tier III |
| 16 |
NC_000010.11 |
10 |
63987735 |
+ |
LOC124902439 |
NNNACCCCTAAGATGAATGG |
NGG |
2 |
0.3667 |
Tier III |
| 17 |
NC_000003.12 |
3 |
61342114 |
- |
LOC105377114 |
NNNACCGCTAAGAAAAATGG |
NCG |
2 |
0.1008 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
103568092 |
+ |
Chl1 |
NNNACCACTAAGAAAAATGG |
NGG |
2 |
0.9412 |
Tier II |
| 2 |
NC_000067.6 |
1 |
164134074 |
- |
Selp |
NNNACTGCTAAGAAAAATGG |
NGG |
2 |
0.8739 |
Tier II |
| 3 |
NC_000069.6 |
3 |
89582552 |
- |
Kcnn3 |
NNNACAGCAAAGAAGAATGG |
NGG |
2 |
0.8622 |
Tier II |
| 4 |
NC_000079.6 |
13 |
41016775 |
+ |
Tmem14c |
NNNGACGCTAAGAAGAATGG |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000075.6 |
9 |
34791274 |
- |
Kirrel3 |
NNNATGGCTAAGAAGAATGG |
NGG |
2 |
0.3182 |
Tier II |
| 6 |
NC_000068.7 |
2 |
18210895 |
+ |
Dnajc1 |
NNNACAGCTATGAAGAATGG |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000068.7 |
2 |
18210895 |
+ |
Mllt10 |
NNNACAGCTATGAAGAATGG |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000076.6 |
10 |
110346154 |
- |
Nav3 |
NNNACCCCTAAGAAGAAGGG |
NGG |
2 |
0.2292 |
Tier II |
| 9 |
NC_000074.6 |
8 |
115183899 |
- |
Wwox |
NNNACTGCTAAGAAGAATGG |
NTG |
2 |
0.0362 |
Tier II |
| 10 |
NC_000070.6 |
4 |
86521616 |
+ |
Saxo1 |
NNNACGGCTAAGAAGAATGG |
NGC |
2 |
0.0111 |
Tier II |
| 11 |
NC_000072.6 |
6 |
94057296 |
+ |
Magi1 |
NNNACCGCTATGAAGAATGG |
NGT |
2 |
0.005 |
Tier II |
| 12 |
NC_000067.6 |
1 |
90443548 |
- |
Gm34992 |
NNNCCAGCTAAGAAGAATGG |
NGG |
2 |
0.3277 |
Tier III |
Other clones with same target sequence:
(none)
