Construct: sgRNA BRDN0001149148
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATGGTCAACTACATCCAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TWF2 (11344)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77343
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
52230875 |
- |
TWF2 |
NNNGGTCAACTACATCCAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
918664 |
+ |
KISS1R |
NNNCGTCAACTACATCCAGC |
NGG |
2 |
0.1203 |
Tier I |
| 3 |
NC_000022.11 |
22 |
29359928 |
- |
AP1B1 |
NNNGGTCAACTGCATGCAGA |
NGG |
2 |
0.1111 |
Tier I |
| 4 |
NC_000004.12 |
4 |
107627120 |
+ |
PAPSS1 |
NNNAGTCAACTACATACAGA |
NGG |
2 |
0.9 |
Tier II |
| 5 |
NC_000012.12 |
12 |
54247179 |
- |
CBX5 |
NNNAGTCATCTACATCCAGA |
NGG |
2 |
0.54 |
Tier II |
| 6 |
NC_000002.12 |
2 |
148912906 |
- |
KIF5C |
NNNAGTCAACTACATCTAGA |
NGG |
2 |
0.42 |
Tier II |
| 7 |
NC_000002.12 |
2 |
148912906 |
- |
LOC101928553 |
NNNAGTCAACTACATCTAGA |
NGG |
2 |
0.42 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
47703639 |
+ |
Frs3 |
NNNGCTCAACTACATCCAGG |
NGG |
2 |
0.6008 |
Tier I |
| 2 |
NC_000075.6 |
9 |
106213059 |
+ |
Twf2 |
NNNGGTCAACTATATCCAGC |
NGG |
2 |
0.1591 |
Tier I |
| 3 |
NC_000076.6 |
10 |
79919547 |
+ |
Kiss1r |
NNNCGTCAACTACATCCAGC |
NGG |
2 |
0.1203 |
Tier I |
| 4 |
NC_000072.6 |
6 |
107552862 |
- |
Lrrn1 |
NNNGATCAACTACATCCAGA |
NGG |
1 |
0.8667 |
Tier II |
| 5 |
NC_000081.6 |
15 |
92701975 |
- |
Pdzrn4 |
NNNGGACAACTAAATCCAGA |
NGG |
2 |
0.3333 |
Tier II |
| 6 |
NC_000076.6 |
10 |
24927035 |
+ |
Arg1 |
NNNGGTGATCTACATCCAGA |
NGG |
2 |
0.2824 |
Tier II |
| 7 |
NC_000076.6 |
10 |
123070238 |
+ |
Mon2 |
NNNGTTCATCTACATCCAGA |
NGG |
2 |
0.18 |
Tier II |
| 8 |
NC_000071.6 |
5 |
19990440 |
+ |
Magi2 |
NNNGGTCAGCTACATCCAGA |
NAG |
2 |
0.1728 |
Tier II |
| 9 |
NC_000076.6 |
10 |
5223408 |
- |
Syne1 |
NNNGGTCATCTCCATCCAGA |
NGG |
2 |
0.1579 |
Tier II |
| 10 |
NC_000067.6 |
1 |
153007077 |
+ |
Nmnat2 |
NNNGGTCAGCTACATCCAGA |
NGA |
2 |
0.0463 |
Tier II |
| 11 |
NC_000076.6 |
10 |
24927035 |
+ |
Gm40616 |
NNNGGTGATCTACATCCAGA |
NGG |
2 |
0.2824 |
Tier III |
| 12 |
NC_000069.6 |
3 |
52656442 |
+ |
Gm30235 |
NNNGGTAAACTCCATCCAGA |
NGG |
2 |
0.1974 |
Tier III |
| 13 |
NC_000075.6 |
9 |
48454936 |
- |
Gm39335 |
NNNGCTCAACTACATCCAGA |
NGA |
2 |
0.0546 |
Tier III |
Other clones with same target sequence:
(none)
