Construct: sgRNA BRDN0001149157
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGCTATGACCATTAGCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CASK (8573)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77401
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
41557067 |
+ |
CASK |
NNNGCTATGACCATTAGCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
63553969 |
- |
USP3 |
NNNGCTATGACCATTAACTA |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000016.10 |
16 |
67777995 |
+ |
RANBP10 |
NNNGCTAAGACCATTAGATG |
NGG |
2 |
0.4308 |
Tier II |
| 4 |
NC_000014.9 |
14 |
21487727 |
- |
TOX4 |
NNNGCCATGCCCATTAGCTG |
NGG |
2 |
0.303 |
Tier II |
| 5 |
NC_000012.12 |
12 |
119857222 |
- |
CIT |
NNNTTTATGACCATTAGCTG |
NGG |
2 |
0.2314 |
Tier II |
| 6 |
NC_000005.10 |
5 |
9328952 |
- |
SEMA5A |
NNNGCTCTGAGCATTAGCTG |
NGG |
2 |
0.1094 |
Tier II |
| 7 |
NC_000011.10 |
11 |
90764841 |
- |
DISC1FP1 |
NNNGTTATGACAATTAGCTG |
NGG |
2 |
0.4545 |
Tier III |
| 8 |
NC_000008.11 |
8 |
75240476 |
- |
CASC9 |
NNNGCTATGACCTTCAGCTG |
NGG |
2 |
0.0818 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
7702252 |
- |
Lats1 |
NNNGCTTTGTCCATTAGCTG |
NGG |
2 |
0.386 |
Tier I |
| 2 |
NC_000086.7 |
X |
13552408 |
+ |
Cask |
NNNGCTATGACCATTAGCAG |
NGG |
1 |
0.2857 |
Tier I |
| 3 |
NC_000076.6 |
10 |
63397316 |
+ |
Dnajc12 |
NNNGCTATGACCATTGGCGG |
NGG |
2 |
0.0481 |
Tier I |
| 4 |
NC_000072.6 |
6 |
112898952 |
+ |
Srgap3 |
NNNGCTATGGCCATTAGCTG |
NGG |
1 |
0.5556 |
Tier II |
| 5 |
NC_000076.6 |
10 |
111191430 |
- |
Osbpl8 |
NNNGCTATGACAATTAGATG |
NGG |
2 |
0.3846 |
Tier II |
| 6 |
NC_000077.6 |
11 |
35141698 |
- |
Slit3 |
NNNGTTATTACCATTAGCTG |
NGG |
2 |
0.3394 |
Tier II |
| 7 |
NC_000081.6 |
15 |
11280180 |
+ |
Adamts12 |
NNNGCTACGACCTTTAGCTG |
NGG |
2 |
0.3 |
Tier II |
| 8 |
NC_000083.6 |
17 |
68674049 |
- |
L3mbtl4 |
NNNGCTATCCCCATTAGCTG |
NGG |
2 |
0.1795 |
Tier II |
| 9 |
NC_000075.6 |
9 |
40454532 |
- |
Gramd1b |
NNNGGTATGACCATTAGCAG |
NGG |
2 |
0.1714 |
Tier II |
| 10 |
NC_000078.6 |
12 |
109060015 |
+ |
Begain |
NNNGCTATGACTCTTAGCTG |
NGG |
2 |
0.1134 |
Tier II |
| 11 |
NC_000070.6 |
4 |
148907214 |
+ |
Casz1 |
NNNGCTGTGACCATTAGCTG |
NCG |
2 |
0.0756 |
Tier II |
| 12 |
NC_000079.6 |
13 |
76469702 |
- |
Mctp1 |
NNNCCTATGACCAGTAGCTG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000072.6 |
6 |
54582901 |
+ |
Fkbp14 |
NNNGCTGTGACCATTTGCTG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000078.6 |
12 |
114501389 |
- |
Igh |
NNNGCTCTGACCATTATCTG |
NGG |
2 |
0.1094 |
Tier III |
| 15 |
NC_000073.6 |
7 |
94138846 |
- |
Gm32647 |
NNNGCTTTGACCATGAGCTG |
NGG |
2 |
0.0219 |
Tier III |
Other clones with same target sequence:
(none)
