Construct: sgRNA BRDN0001149160
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAGCATTCATAGACGGCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK2 (8445)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77537
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
67657307 |
+ |
DYRK2 |
NNNGCATTCATAGACGGCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
38123073 |
+ |
ACAA1 |
NNNGTATTCAAAGACGGCAG |
NGG |
2 |
0.4773 |
Tier I |
| 3 |
NC_000004.12 |
4 |
109649577 |
- |
MCUB |
NNNGAATTAATAGACGGCAG |
NGG |
2 |
0.4898 |
Tier II |
| 4 |
NC_000003.12 |
3 |
38123073 |
+ |
DLEC1 |
NNNGTATTCAAAGACGGCAG |
NGG |
2 |
0.4773 |
Tier II |
| 5 |
NC_000015.10 |
15 |
67724763 |
+ |
MAP2K5 |
NNNGCATACATAGAAGGCAG |
NGG |
2 |
0.1778 |
Tier II |
| 6 |
NC_000017.11 |
17 |
48283507 |
- |
SKAP1 |
NNNGCATCCATAGATGGCAG |
NGG |
2 |
0.0667 |
Tier II |
| 7 |
NC_000016.10 |
16 |
81638276 |
+ |
CMIP |
NNNGCATTCACAGATGGCAG |
NGG |
2 |
0.0444 |
Tier II |
| 8 |
NC_000014.9 |
14 |
71970584 |
+ |
RGS6 |
NNNGAATTCATAGAGGGCAG |
NGG |
2 |
0.0286 |
Tier II |
| 9 |
NC_000004.12 |
4 |
52923589 |
+ |
SCFD2 |
NNNGGATTCATAGACTGCAG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000012.12 |
12 |
87333816 |
- |
LOC107984478 |
NNNGCTTTCATAGACGGCAA |
NGG |
2 |
0.6696 |
Tier III |
| 11 |
NC_000001.11 |
1 |
211537763 |
- |
LOC107985260 |
NNNGCATCCAGAGACGGCAG |
NGG |
2 |
0.4 |
Tier III |
| 12 |
NC_000001.11 |
1 |
211537763 |
- |
LOC107985261 |
NNNGCATCCAGAGACGGCAG |
NGG |
2 |
0.4 |
Tier III |
| 13 |
NC_000016.10 |
16 |
81638276 |
+ |
LOC105371362 |
NNNGCATTCACAGATGGCAG |
NGG |
2 |
0.0444 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
37931443 |
- |
Rreb1 |
NNNGCAGTCATAGAGGGCAG |
NGG |
2 |
0.0294 |
Tier I |
| 2 |
NC_000073.6 |
7 |
73520505 |
+ |
Chd2 |
NNNGCATTCAAAGACAGCAG |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000085.6 |
19 |
3654997 |
- |
Lrp5 |
NNNGCAGTTATAGACGGCAG |
NGG |
2 |
0.5147 |
Tier II |
| 4 |
NC_000071.6 |
5 |
145108577 |
+ |
Arpc1a |
NNNGCATTTAGAGACGGCAG |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000082.6 |
16 |
55963597 |
+ |
Gm6958 |
NNNGCATTCATAGACGGCTC |
NGG |
2 |
0.2308 |
Tier II |
| 6 |
NC_000081.6 |
15 |
88722028 |
- |
Brd1 |
NNNGCATTCATAGCCGGCAG |
NGG |
1 |
0.2143 |
Tier II |
| 7 |
NC_000076.6 |
10 |
99131340 |
+ |
Poc1b |
NNNGCATTCAGACACGGCAG |
NGG |
2 |
0.1684 |
Tier II |
| 8 |
NC_000073.6 |
7 |
49573949 |
- |
Nav2 |
NNNGCATGCATAGATGGCAG |
NGG |
2 |
0.0489 |
Tier II |
| 9 |
NC_000082.6 |
16 |
22070550 |
- |
Igf2bp2 |
NNNGCATTCATAGGGGGCAG |
NGG |
2 |
0.0233 |
Tier II |
| 10 |
NC_000075.6 |
9 |
34846361 |
- |
Kirrel3 |
NNNGCATTCAGAGAGGGCAG |
NGG |
2 |
0.02 |
Tier II |
| 11 |
NC_000073.6 |
7 |
130652339 |
- |
Tacc2 |
NNNCCATTCATAGACTGCAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000072.6 |
6 |
55249448 |
+ |
Mindy4 |
NNNGCATTCAGAGACTGCAG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000081.6 |
15 |
95716458 |
+ |
Gm8843 |
NNNGCATTCAGAGACAGCAG |
NGG |
2 |
0.4 |
Tier III |
| 14 |
NC_000075.6 |
9 |
60531280 |
- |
9230112J17Rik |
NNNGCATTCAAAGGCGGCAG |
NGG |
2 |
0.35 |
Tier III |
| 15 |
NC_000073.6 |
7 |
78699086 |
+ |
E430016F16Rik |
NNNGCATTCATAGACGGGAA |
NGG |
2 |
0.125 |
Tier III |
| 16 |
NC_000068.7 |
2 |
160284923 |
- |
Gm46773 |
NNNGCATTCATAGACGACAG |
NTG |
2 |
0.0364 |
Tier III |
| 17 |
NC_000075.6 |
9 |
34846361 |
- |
Gm33561 |
NNNGCATTCAGAGAGGGCAG |
NGG |
2 |
0.02 |
Tier III |
Other clones with same target sequence:
(none)
