Construct: sgRNA BRDN0001149166

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGTGGCAGTGACCACAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IP6K1 (9807)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76136

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 49738252 - IP6K1 NNNTGGCAGTGACCACAAGG NGG 0 1.0 Tier I
2 NC_000008.11 8 133022101 - TG NNNTGGCAGTGACCACGATG NGG 2 0.1176 Tier I
3 NC_000007.14 7 99912991 - TRIM4 NNNTGACAGTGACCACAAAG NGG 2 0.7143 Tier II
4 NC_000011.10 11 118351348 - CD3G NNNTGGAAGTGACCACAAGA NGG 2 0.7031 Tier II
5 NC_000023.11 X 40144829 - BCOR NNNTGGCAGTGATCAAAAGG NGG 2 0.7 Tier II
6 NC_000015.10 15 26623282 + GABRB3 NNNGGGCAGTGACCACAAGG NGG 1 0.625 Tier II
7 NC_000008.11 8 22840765 - PEBP4 NNNTGGCAGAGATCACAAGG NGG 2 0.6 Tier II
8 NC_000011.10 11 43728023 + HSD17B12 NNNTGGCAGTAACCACATGG NGG 2 0.5 Tier II
9 NC_000014.9 14 89336493 + FOXN3 NNNAGACAGTGACCACAAGG NGG 2 0.4762 Tier II
10 NC_000010.11 10 88744218 - LIPK NNNTGGCAGTGACCACAGGG NGG 1 0.4 Tier II
11 NC_000017.11 17 28555104 + PIGS NNNTGGCTGAGACCACAAGG NGG 2 0.3673 Tier II
12 NC_000009.12 9 121926402 - TTLL11 NNNTGGCAGTGAACACAAGA NGG 2 0.3606 Tier II
13 NC_000023.11 X 153873395 + L1CAM NNNTGGCAGTGGCAACAAGG NGG 2 0.2528 Tier II
14 NC_000009.12 9 134338132 + RXRA NNNGGGCAGTGACCACAGGG NGG 2 0.25 Tier II
15 NC_000006.12 6 42372843 + TRERF1 NNNTGGCACTGACCACAGGG NGG 2 0.2154 Tier II
16 NC_000002.12 2 66438196 + MEIS1 NNNTGGCAGTGCCCGCAAGG NGG 2 0.1711 Tier II
17 NC_000011.10 11 112998600 - NCAM1 NNNTGGCAGTGAACACAAGC NGG 2 0.1648 Tier II
18 NC_000014.9 14 72024181 + RGS6 NNNTGGTAGTGACCACCAGG NGG 2 0.1434 Tier II
19 NC_000021.9 21 42686474 + PDE9A NNNTGGCTGTGTCCACAAGG NGG 2 0.1429 Tier II
20 NC_000007.14 7 100373429 + PILRA NNNTGGCACTGACCACAAGG NAG 2 0.1396 Tier II
21 NC_000019.10 19 10255556 + MRPL4 NNNTGGCAGGGACCACAAGG NAG 2 0.1296 Tier II
22 NC_000019.10 19 18874671 + GDF1 NNNTGGGAGTGACCACAAGG NAG 2 0.122 Tier II
23 NC_000019.10 19 18874671 + CERS1 NNNTGGGAGTGACCACAAGG NAG 2 0.122 Tier II
24 NC_000019.10 19 6216147 + MLLT1 NNNTGCCAGTGACCACGAGG NGG 2 0.1203 Tier II
25 NC_000016.10 16 13216889 - SHISA9 NNNTAGCAGTGACCACTAGG NGG 2 0.1156 Tier II
26 NC_000016.10 16 79505867 + MAF NNNTGGCAGTGACCAGAATG NGG 2 0.1026 Tier II
27 NC_000002.12 2 101891655 + MAP4K4 NNNTGGCAGTGGGCACAAGG NGG 2 0.0985 Tier II
28 NC_000016.10 16 24306201 - CACNG3 NNNAGGCAGTGACCACACGG NGG 2 0.0907 Tier II
29 NC_000011.10 11 131428540 - NTM NNNTGGGAGTGACCACGAGG NGG 2 0.083 Tier II
30 NC_000017.11 17 76684848 - MXRA7 NNNTGGCACTGACCAGAAGG NGG 2 0.0828 Tier II
31 NC_000004.12 4 184125961 - ENPP6 NNNTGGCACTGAGCACAAGG NGG 2 0.0734 Tier II
32 NC_000018.10 18 34867790 + DTNA NNNTGGCAGTGCCCCCAAGG NGG 2 0.0718 Tier II
33 NC_000003.12 3 123834834 + MYLK NNNTGGCAGTGACCACAAGA NTG 2 0.0365 Tier II
34 NC_000023.11 X 70550008 + TEX11 NNNTGGCAGTGGCCACAAGG NTG 2 0.0281 Tier II
35 NC_000002.12 2 20589234 - HS1BP3 NNNTGGCAGTGACCACAAGG NGT 1 0.0161 Tier II
36 NC_000009.12 9 134374338 - RXRA NNNTGGCAGTGACCACCAGG NTG 2 0.0069 Tier II
37 NC_000005.10 5 21648656 + LOC105374685 NNNTGGTAGTGACCACAAGG NGG 1 0.8125 Tier III
38 NC_000019.10 19 42632940 - CEACAMP1 NNNTGGGAGTGAACACAAGG NGG 2 0.181 Tier III
39 NC_000019.10 19 42632940 - LIPE-AS1 NNNTGGGAGTGAACACAAGG NGG 2 0.181 Tier III
40 NC_000018.10 18 76213316 + LINC01893 NNNTGGCAGGGACCATAAGG NGG 2 0.1538 Tier III
41 NC_000016.10 16 79505867 + LOC124903729 NNNTGGCAGTGACCAGAATG NGG 2 0.1026 Tier III
42 NC_000008.11 8 83929310 - LOC124901968 NNNTGGTAGTGACCACAAGG NGA 2 0.0564 Tier III
43 NC_000023.11 X 76738971 + MIR325HG NNNTGGCAGTGACTACAAGG NGA 2 0.0509 Tier III
44 NC_000023.11 X 33893145 - LOC105373153 NNNTGGCAGTGGCCACAAGG NGA 2 0.0502 Tier III
45 NC_000023.11 X 128520662 + LOC107985698 NNNTGGCAGTGGCCACAAGG NGA 2 0.0502 Tier III
46 NC_000002.12 2 144906196 - TEX41 NNNTGGCAGTGAGAACAAGG NGG 2 0.0477 Tier III
47 NC_000010.11 10 85464672 + LINC01520 NNNTGGCAGTGACCACAGGG NCG 2 0.0429 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 108032170 + Ip6k1 NNNTGGTAGTGACCACAAGG NGG 1 0.8125 Tier I
2 NC_000071.6 5 137858148 - Pilrb1 NNNTGTCAGTGACCACAGGG NGG 2 0.2667 Tier I
3 NC_000071.6 5 124602036 + Tctn2 NNNTGGAAGTGACCATAAGG NGG 2 0.2308 Tier I
4 NC_000075.6 9 21034719 - Icam5 NNNTGGCAGTGGCCACAAGG NTG 2 0.0281 Tier I
5 NC_000072.6 6 29442217 - Flnc NNNTGACAGAGACCACAAGG NGG 2 0.8571 Tier II
6 NC_000068.7 2 119721992 - Rtf1 NNNTGGCAGAAACCACAAGG NGG 2 0.8571 Tier II
7 NC_000076.6 10 117055905 - Cct2 NNNTCCCAGTGACCACAAGG NGG 2 0.5357 Tier II
8 NC_000078.6 12 112761240 + Pld4 NNNTCCCAGTGACCACAAGG NGG 2 0.5357 Tier II
9 NC_000079.6 13 20940588 + Aoah NNNTGGCAATGGCCACAAGG NGG 2 0.4643 Tier II
10 NC_000068.7 2 60339788 + Ly75 NNNTGACAGTGACCACAACG NGG 2 0.4483 Tier II
11 NC_000082.6 16 5181621 + Sec14l5 NNNTGACAGTCACCACAAGG NGG 2 0.4286 Tier II
12 NC_000083.6 17 48393712 + Nfya NNNTGGCTGTGACCACAAGG NGG 1 0.4286 Tier II
13 NC_000075.6 9 110679122 + Ccdc12 NNNCGGCAGGGACCACAAGG NGG 2 0.3235 Tier II
14 NC_000070.6 4 111373220 + Agbl4 NNNAGTCAGTGACCACAAGG NGG 2 0.3175 Tier II
15 NC_000084.6 18 61318396 + Ppargc1b NNNTGGCAGTCACCACAATG NGG 2 0.2857 Tier II
16 NC_000068.7 2 139966512 - Tasp1 NNNTGGAAGTGTCCACAAGG NGG 2 0.25 Tier II
17 NC_000068.7 2 18989262 - Pip4k2a NNNTGGCAGGGACCACATGG NGG 2 0.25 Tier II
18 NC_000078.6 12 82701096 + Rgs6 NNNTGGTAGTGACCACCAGG NGG 2 0.1434 Tier II
19 NC_000086.7 X 152148021 - Iqsec2 NNNTAGCAGTGACCAGAAGG NGG 2 0.1333 Tier II
20 NC_000077.6 11 8941446 - Pkd1l1 NNNTGGCAGTGACCACCAGT NGG 2 0.1235 Tier II
21 NC_000077.6 11 88163271 + Cuedc1 NNNTGGCAGGGACCAGAAGG NGG 2 0.0769 Tier II
22 NC_000069.6 3 139257085 + Stpg2 NNNAGGCAGTGACCACTAGG NGG 2 0.0635 Tier II
23 NC_000084.6 18 75251892 - Dym NNNTGGCAGTGAGCACAACG NGG 2 0.0611 Tier II
24 NC_000074.6 8 58090699 - Galntl6 NNNTGGCAGTGAACAGAAGG NGG 2 0.0592 Tier II
25 NC_000072.6 6 118467514 + Zfp9 NNNTGGCAGTGACCATTAGG NGG 2 0.041 Tier II
26 NC_000083.6 17 13876959 + Afdn NNNTGGCAGTGAGCTCAAGG NGG 2 0.0273 Tier II
27 NC_000073.6 7 120105169 - Tmem159 NNNTGGCAGTGACCACAAGG NGC 1 0.0222 Tier II
28 NC_000070.6 4 102985655 + Tctex1d1 NNNTGGCAGTGCCCACAAGG NGA 2 0.0183 Tier II
29 NC_000078.6 12 83277007 + Dpf3 NNNTGGCAGTGTCCACAAGG NTG 2 0.013 Tier II
30 NC_000068.7 2 179949631 + Taf4 NNNTGGCAGTGCCCACAAGG NGC 2 0.0058 Tier II
31 NC_000067.6 1 54806376 + Ankrd44 NNNTGGCAGTGAGCACAAGG NTG 2 0.0053 Tier II
32 NC_000079.6 13 20940588 + Gm32242 NNNTGGCAATGGCCACAAGG NGG 2 0.4643 Tier III
33 NC_000079.6 13 69691281 + Gm35618 NNNGGCCAGTGACCACAAGG NGG 2 0.4261 Tier III
34 NC_000079.6 13 69691281 + Gm40993 NNNGGCCAGTGACCACAAGG NGG 2 0.4261 Tier III
35 NC_000071.6 5 137858148 - Gm36551 NNNTGTCAGTGACCACAGGG NGG 2 0.2667 Tier III
36 NC_000070.6 4 102985655 + Gm12709 NNNTGGCAGTGCCCACAAGG NGA 2 0.0183 Tier III
37 NC_000078.6 12 83277007 + Gm18872 NNNTGGCAGTGTCCACAAGG NTG 2 0.013 Tier III
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Other clones with same target sequence:

(none)