Construct: sgRNA BRDN0001149175

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATGGCCGCACCATCCACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGFRL1 (53834)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76976

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 1022336 + FGFRL1 NNNGGCCGCACCATCCACAG NGG 0 1.0 Tier I
2 NC_000009.12 9 123557650 + DENND1A NNNGGCCACACCATCCCCAG NGG 2 0.1765 Tier I
3 NC_000008.11 8 97851428 - LAPTM4B NNNGGCAGCACCATTCACAG NGG 2 0.05 Tier I
4 NC_000007.14 7 47843645 - PKD1L1 NNNAGCAGCACCATCCACAG NGG 2 0.675 Tier II
5 NC_000004.12 4 161822249 - FSTL5 NNNGGCACCACCATCCACAG NGG 2 0.4615 Tier II
6 NC_000016.10 16 2769474 + SRRM2 NNNGGCTGCGCCATCCACAG NGG 2 0.4514 Tier II
7 NC_000022.11 22 26453640 + HPS4 NNNGGCGGGACCATCCACAG NGG 2 0.2913 Tier II
8 NC_000011.10 11 64853378 - EHD1 NNNGGACGCACCCTCCACAG NGG 2 0.1955 Tier II
9 NC_000017.11 17 21314526 - MAP2K3 NNNGGCAGCAGCATCCACAG NGG 2 0.1875 Tier II
10 NC_000010.11 10 21610387 + MLLT10 NNNGGCCACACCATCCAGAG NGG 2 0.1333 Tier II
11 NC_000002.12 2 98234527 + VWA3B NNNGGCAGCACCATCCTCAG NGG 2 0.1 Tier II
12 NC_000010.11 10 35305843 - CCNY NNNGGCAGCACCATCCACAG NGA 2 0.0521 Tier II
13 NC_000009.12 9 98379049 + GABBR2 NNNGGCCGCAGCATCCTCAG NGG 2 0.0333 Tier II
14 NC_000013.11 13 49677515 + EBPL NNNGGCTGCACCATCCACAG NTG 2 0.0317 Tier II
15 NC_000006.12 6 150682134 - PLEKHG1 NNNGGCCTCACCATGCACAG NGG 2 0.0313 Tier II
16 NC_000016.10 16 5027689 + NAGPA NNNGGCCGCAGCAGCCACAG NGG 2 0.0 Tier II
17 NC_000011.10 11 67377537 + LOC100130987 NNNGGCTTCACCATCCACAG NGG 2 0.5078 Tier III
18 NC_000017.11 17 21773459 + LOC100996792 NNNGGCAGCAGCATCCACAG NGG 2 0.1875 Tier III
19 NC_000020.11 20 21674861 - LINC01726 NNNGGCCCCACCATCCCCAG NGG 2 0.1086 Tier III
20 NC_000010.11 10 35305843 - LOC107984220 NNNGGCAGCACCATCCACAG NGA 2 0.0521 Tier III
21 NC_000001.11 1 153379852 + LAPTM4BP1 NNNGGCAGCACCATTCACAG NGG 2 0.05 Tier III
22 NC_000003.12 3 72884636 - LAPTM4BP2 NNNGGCAGCACCATTCACAG NGG 2 0.05 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 108703419 + Fgfrl1 NNNGGCCGCACAATCCACAG NGG 1 0.7143 Tier I
2 NC_000070.6 4 142010058 - Fhad1 NNNGTCAGCACCATCCACAG NGG 2 0.225 Tier I
3 NC_000068.7 2 58024178 - Galnt5 NNNGGTCGCACCATCCACAG NGG 1 0.9286 Tier II
4 NC_000074.6 8 111820782 - Cfdp1 NNNGGCAGCACCATCCACAG NGG 1 0.75 Tier II
5 NC_000079.6 13 15557906 - Gli3 NNNAGCTGCACCATCCACAG NGG 2 0.7313 Tier II
6 NC_000071.6 5 128002221 + Tmem132d NNNGGTAGCACCATCCACAG NGG 2 0.6964 Tier II
7 NC_000086.7 X 20911485 - Syn1 NNNGGTAGCACCATCCACAG NGG 2 0.6964 Tier II
8 NC_000074.6 8 111800802 + Cfdp1 NNNGGCCTCTCCATCCACAG NGG 2 0.5515 Tier II
9 NC_000077.6 11 93198051 + Car10 NNNGGCAGCACCATCCACAT NGG 2 0.525 Tier II
10 NC_000075.6 9 21646195 + Smarca4 NNNGGCTGGACCATCCACAG NGG 2 0.503 Tier II
11 NC_000074.6 8 117333097 + Cmip NNNGGCTCCACCATCCACAG NGG 2 0.5 Tier II
12 NC_000071.6 5 127513300 - Tmem132c NNNGGCAGCACCAACCACAG NGG 2 0.4643 Tier II
13 NC_000068.7 2 35041476 - Hc NNNGGTGGCACCATCCACAG NGG 2 0.437 Tier II
14 NC_000069.6 3 9695598 - Pag1 NNNGGTGGCACCATCCACAG NGG 2 0.437 Tier II
15 NC_000069.6 3 13955796 + Ralyl NNNGGTGGCACCATCCACAG NGG 2 0.437 Tier II
16 NC_000076.6 10 43921666 - Rtn4ip1 NNNGGTGGCACCATCCACAG NGG 2 0.437 Tier II
17 NC_000078.6 12 29728913 - Myt1l NNNGGCGGCACCATCCATAG NGG 2 0.3025 Tier II
18 NC_000084.6 18 42841168 + Ppp2r2b NNNGGCGGCACCAACCACAG NGG 2 0.2913 Tier II
19 NC_000067.6 1 134676038 + Syt2 NNNGGCAGCACCACCCACAG NGG 2 0.2143 Tier II
20 NC_000071.6 5 30446393 + Otof NNNGGCTGCAGCATCCACAG NGG 2 0.2031 Tier II
21 NC_000068.7 2 35769977 - Ttll11 NNNGGCCCCACCACCCACAG NGG 2 0.1758 Tier II
22 NC_000071.6 5 125653137 + Tmem132b NNNTGCGGCACCATCCACAG NGG 2 0.1711 Tier II
23 NC_000070.6 4 120705010 + Kcnq4 NNNGGCCTCACCATACACAG NGG 2 0.1389 Tier II
24 NC_000074.6 8 112758850 + Cntnap4 NNNGGCGGCACCACCCACAG NGG 2 0.1345 Tier II
25 NC_000071.6 5 111737333 + E130006D01Rik NNNGGTGGCACCATCCACAG NGG 2 0.437 Tier III
26 NC_000081.6 15 66888058 + Gm46514 NNNGACGGCACCATCCACAG NGG 2 0.4078 Tier III
27 NC_000078.6 12 34999036 - Gm40385 NNNGGCTGCACGATCCACAG NGG 2 0.3611 Tier III
28 NC_000071.6 5 127038253 - 4933438B17Rik NNNGTCCTCACCATCCACAG NGG 2 0.1875 Tier III
29 NC_000084.6 18 55934356 + Gm52332 NNNGGCGGCACCATCCACAG NAG 2 0.122 Tier III
30 NC_000076.6 10 60936838 - Pldi NNNGGCTGCACCATCCAGAG NGG 2 0.1083 Tier III
31 NC_000076.6 10 60936838 - Gm26947 NNNGGCTGCACCATCCAGAG NGG 2 0.1083 Tier III
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Other clones with same target sequence:

(none)