Construct: sgRNA BRDN0001149178
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACCTGACAGCATCGAGCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ABCC1 (4363)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76860
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
16071711 |
+ |
ABCC1 |
NNNCTGACAGCATCGAGCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
31897714 |
+ |
TTLL9 |
NNNCTGACAGCACCGAGCGG |
NGG |
2 |
0.6037 |
Tier II |
| 3 |
NC_000001.11 |
1 |
46938332 |
- |
CYP4A11 |
NNNCTGGCAGCATTGAGCGA |
NGG |
2 |
0.5176 |
Tier II |
| 4 |
NC_000011.10 |
11 |
99516589 |
+ |
CNTN5 |
NNNCTGAGAGCATCGAGTGA |
NGG |
2 |
0.4133 |
Tier II |
| 5 |
NC_000005.10 |
5 |
103910233 |
- |
LOC105379107 |
NNNCTGACAGCATCAAGCGA |
NGC |
2 |
0.0209 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
42003995 |
+ |
Sorl1 |
NNNCTCACAGCATCCAGCGA |
NGG |
2 |
0.186 |
Tier I |
| 2 |
NC_000079.6 |
13 |
93852672 |
- |
Arsb |
NNNCTGACAGCAACCAGCGA |
NGG |
2 |
0.1888 |
Tier II |
| 3 |
NC_000067.6 |
1 |
158447565 |
- |
Astn1 |
NNNCTGACAGCAGCGAGCCA |
NGG |
2 |
0.1169 |
Tier II |
| 4 |
NC_000071.6 |
5 |
104173326 |
+ |
Dspp |
NNNCTGCCAGCATCGAGCGA |
NCG |
2 |
0.0469 |
Tier II |
| 5 |
NC_000078.6 |
12 |
89287257 |
+ |
Nrxn3 |
NNNCTGACAGCATCTGGCGA |
NGG |
2 |
0.0275 |
Tier II |
| 6 |
NC_000074.6 |
8 |
81542906 |
- |
Inpp4b |
NNNCTGACAGCATGGAGCTA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000067.6 |
1 |
158447565 |
- |
Gm37679 |
NNNCTGACAGCAGCGAGCCA |
NGG |
2 |
0.1169 |
Tier III |
| 8 |
NC_000078.6 |
12 |
45467350 |
- |
Gm33680 |
NNNCTGACAGCATGGAGCAA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
