Construct: sgRNA BRDN0001149181
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTAGACCTACTATTCAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3C2A (5286)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76414
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
17169329 |
- |
PIK3C2A |
NNNAGACCTACTATTCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
32757716 |
- |
HLA-DQB2 |
NNNAGACCCTCTATTCAGAG |
NGG |
2 |
0.8145 |
Tier II |
| 3 |
NC_000017.11 |
17 |
4530445 |
+ |
SPNS2 |
NNNAGACCCACTATACAGAG |
NGG |
2 |
0.5344 |
Tier II |
| 4 |
NC_000019.10 |
19 |
44057622 |
+ |
ZNF223 |
NNNAGAACTATTATTCAGAG |
NGG |
2 |
0.2308 |
Tier II |
| 5 |
NC_000009.12 |
9 |
76885614 |
+ |
PRUNE2 |
NNNAGACTTACTCTTCAGAG |
NGG |
2 |
0.1842 |
Tier II |
| 6 |
NC_000023.11 |
X |
75432662 |
+ |
ZDHHC15 |
NNNAGACCTACTATACAGAG |
NAG |
2 |
0.1501 |
Tier II |
| 7 |
NC_000001.11 |
1 |
9051028 |
- |
SLC2A5 |
NNNTGACCTACTATTCTGAG |
NGG |
2 |
0.0848 |
Tier II |
| 8 |
NC_000009.12 |
9 |
127129681 |
- |
RALGPS1 |
NNNAGACCTACTAGTCAGAA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000008.11 |
8 |
113193231 |
+ |
CSMD3 |
NNNAGACCTACTATTCAGAG |
NCT |
2 |
0.0 |
Tier II |
| 10 |
NC_000013.11 |
13 |
87356836 |
- |
LOC105370302 |
NNNAGAGCTACAATTCAGAG |
NGG |
2 |
0.3765 |
Tier III |
| 11 |
NC_000004.12 |
4 |
148862655 |
- |
LOC107986195 |
NNNAGCCCTACTATTCAGAC |
NGG |
2 |
0.1948 |
Tier III |
| 12 |
NC_000006.12 |
6 |
33924583 |
+ |
LOC124901307 |
NNNAGACCTACTATGCAGAG |
NGG |
1 |
0.05 |
Tier III |
| 13 |
NC_000002.12 |
2 |
66995904 |
+ |
LOC105374785 |
NNNAGACCTATTATTCAGAG |
NGA |
2 |
0.0214 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
129359506 |
- |
Clec12a |
NNNAGACTTACTCTTCAGAG |
NGG |
2 |
0.1842 |
Tier I |
| 2 |
NC_000076.6 |
10 |
105467438 |
+ |
Tmtc2 |
NNNAGACCAACTATTCAGAT |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000068.7 |
2 |
23357235 |
- |
Nxph2 |
NNNAGACATACAATTCAGAG |
NGG |
2 |
0.52 |
Tier II |
| 4 |
NC_000071.6 |
5 |
8939699 |
- |
Abcb4 |
NNNAGACCTTCTATACAGAG |
NGG |
2 |
0.5108 |
Tier II |
| 5 |
NC_000077.6 |
11 |
94482488 |
+ |
Spata20 |
NNNAGAGCTACTGTTCAGAG |
NGG |
2 |
0.3069 |
Tier II |
| 6 |
NC_000072.6 |
6 |
114035114 |
+ |
Atp2b2 |
NNNAGACCTTCTTTTCAGAG |
NGG |
2 |
0.2647 |
Tier II |
| 7 |
NC_000077.6 |
11 |
62075244 |
- |
Specc1 |
NNNAGTCCTCCTATTCAGAG |
NGG |
2 |
0.2381 |
Tier II |
| 8 |
NC_000077.6 |
11 |
70053234 |
+ |
Asgr1 |
NNNAGAGCTACTATTCAGAC |
NGG |
2 |
0.2017 |
Tier II |
| 9 |
NC_000068.7 |
2 |
17557827 |
+ |
Nebl |
NNNAGACATACTATGCAGAG |
NGG |
2 |
0.0325 |
Tier II |
| 10 |
NC_000072.6 |
6 |
119498741 |
+ |
Wnt5b |
NNNAGACCTACTCTGCAGAG |
NGG |
2 |
0.0105 |
Tier II |
| 11 |
NC_000068.7 |
2 |
23357235 |
- |
Gm34769 |
NNNAGACATACAATTCAGAG |
NGG |
2 |
0.52 |
Tier III |
| 12 |
NC_000071.6 |
5 |
8939699 |
- |
Gm31388 |
NNNAGACCTTCTATACAGAG |
NGG |
2 |
0.5108 |
Tier III |
| 13 |
NC_000072.6 |
6 |
114035114 |
+ |
Gm15083 |
NNNAGACCTTCTTTTCAGAG |
NGG |
2 |
0.2647 |
Tier III |
| 14 |
NC_000082.6 |
16 |
52853897 |
+ |
4930405D01Rik |
NNNAGACCTTCTCTTCAGAG |
NGG |
2 |
0.1858 |
Tier III |
Other clones with same target sequence:
(none)
