Construct: sgRNA BRDN0001149181

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTAGACCTACTATTCAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C2A (5286)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76414

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 17169329 - PIK3C2A NNNAGACCTACTATTCAGAG NGG 0 1.0 Tier I
2 NC_000006.12 6 32757716 - HLA-DQB2 NNNAGACCCTCTATTCAGAG NGG 2 0.8145 Tier II
3 NC_000017.11 17 4530445 + SPNS2 NNNAGACCCACTATACAGAG NGG 2 0.5344 Tier II
4 NC_000019.10 19 44057622 + ZNF223 NNNAGAACTATTATTCAGAG NGG 2 0.2308 Tier II
5 NC_000009.12 9 76885614 + PRUNE2 NNNAGACTTACTCTTCAGAG NGG 2 0.1842 Tier II
6 NC_000023.11 X 75432662 + ZDHHC15 NNNAGACCTACTATACAGAG NAG 2 0.1501 Tier II
7 NC_000001.11 1 9051028 - SLC2A5 NNNTGACCTACTATTCTGAG NGG 2 0.0848 Tier II
8 NC_000009.12 9 127129681 - RALGPS1 NNNAGACCTACTAGTCAGAA NGG 2 0.0 Tier II
9 NC_000008.11 8 113193231 + CSMD3 NNNAGACCTACTATTCAGAG NCT 2 0.0 Tier II
10 NC_000013.11 13 87356836 - LOC105370302 NNNAGAGCTACAATTCAGAG NGG 2 0.3765 Tier III
11 NC_000004.12 4 148862655 - LOC107986195 NNNAGCCCTACTATTCAGAC NGG 2 0.1948 Tier III
12 NC_000006.12 6 33924583 + LOC124901307 NNNAGACCTACTATGCAGAG NGG 1 0.05 Tier III
13 NC_000002.12 2 66995904 + LOC105374785 NNNAGACCTATTATTCAGAG NGA 2 0.0214 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 129359506 - Clec12a NNNAGACTTACTCTTCAGAG NGG 2 0.1842 Tier I
2 NC_000076.6 10 105467438 + Tmtc2 NNNAGACCAACTATTCAGAT NGG 2 0.65 Tier II
3 NC_000068.7 2 23357235 - Nxph2 NNNAGACATACAATTCAGAG NGG 2 0.52 Tier II
4 NC_000071.6 5 8939699 - Abcb4 NNNAGACCTTCTATACAGAG NGG 2 0.5108 Tier II
5 NC_000077.6 11 94482488 + Spata20 NNNAGAGCTACTGTTCAGAG NGG 2 0.3069 Tier II
6 NC_000072.6 6 114035114 + Atp2b2 NNNAGACCTTCTTTTCAGAG NGG 2 0.2647 Tier II
7 NC_000077.6 11 62075244 - Specc1 NNNAGTCCTCCTATTCAGAG NGG 2 0.2381 Tier II
8 NC_000077.6 11 70053234 + Asgr1 NNNAGAGCTACTATTCAGAC NGG 2 0.2017 Tier II
9 NC_000068.7 2 17557827 + Nebl NNNAGACATACTATGCAGAG NGG 2 0.0325 Tier II
10 NC_000072.6 6 119498741 + Wnt5b NNNAGACCTACTCTGCAGAG NGG 2 0.0105 Tier II
11 NC_000068.7 2 23357235 - Gm34769 NNNAGACATACAATTCAGAG NGG 2 0.52 Tier III
12 NC_000071.6 5 8939699 - Gm31388 NNNAGACCTTCTATACAGAG NGG 2 0.5108 Tier III
13 NC_000072.6 6 114035114 + Gm15083 NNNAGACCTTCTTTTCAGAG NGG 2 0.2647 Tier III
14 NC_000082.6 16 52853897 + 4930405D01Rik NNNAGACCTTCTCTTCAGAG NGG 2 0.1858 Tier III
Download CSV

Other clones with same target sequence:

(none)

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