Construct: sgRNA BRDN0001149186
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGAATACGTCCTCCACATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3CG (5294)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76334
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
106869011 |
+ |
PIK3CG |
NNNAATACGTCCTCCACATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
35779263 |
+ |
SPEF2 |
NNNAATACGTCCTCCACCTG |
NAG |
2 |
0.0494 |
Tier I |
| 3 |
NC_000020.11 |
20 |
25324282 |
+ |
ABHD12 |
NNNAACACGACCTCCACATG |
NGG |
2 |
0.7792 |
Tier II |
| 4 |
NC_000016.10 |
16 |
68691629 |
- |
CDH3 |
NNNAATACGGCTTCCACATG |
NGG |
2 |
0.2692 |
Tier II |
| 5 |
NC_000013.11 |
13 |
48385279 |
- |
RB1 |
NNNAATATGTCCTCCACAGG |
NGG |
2 |
0.2188 |
Tier II |
| 6 |
NC_000001.11 |
1 |
67326640 |
- |
IL12RB2 |
NNNAATACGTCCCCCACATG |
NCG |
2 |
0.0846 |
Tier II |
| 7 |
NC_000011.10 |
11 |
73473784 |
+ |
FAM168A |
NNNAATAGGTCCTCCACATG |
NTG |
2 |
0.025 |
Tier II |
| 8 |
NC_000006.12 |
6 |
139040191 |
- |
ABRACL |
NNNAATACATCCTCCACATG |
NGT |
2 |
0.0104 |
Tier II |
| 9 |
NC_000007.14 |
7 |
55687272 |
- |
FKBP9P1 |
NNNAATGTGTCCTCCACATG |
NGG |
2 |
0.6176 |
Tier III |
| 10 |
NC_000015.10 |
15 |
91803678 |
+ |
LOC105370974 |
NNNAATACTTCCTCCACAGG |
NGG |
2 |
0.1333 |
Tier III |
| 11 |
NC_000015.10 |
15 |
35958299 |
- |
LOC105370766 |
NNNAATATGTCCTCCACATG |
NTG |
2 |
0.0341 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
35588847 |
+ |
Acox3 |
NNNAATGCGTCCTCCACATG |
NTG |
2 |
0.0275 |
Tier I |
| 2 |
NC_000073.6 |
7 |
142436254 |
- |
Syt8 |
NNNAATCCGGCCTCCACATG |
NGG |
2 |
0.2188 |
Tier II |
| 3 |
NC_000069.6 |
3 |
93113312 |
- |
Gm35785 |
NNNAATACATACTCCACATG |
NGG |
2 |
0.4821 |
Tier III |
Other clones with same target sequence:
(none)
