Construct: sgRNA BRDN0001149188
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCAGGATGAGACACTACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TBCK (93627)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75567
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
106232971 |
+ |
TBCK |
NNNAGGATGAGACACTACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
3808414 |
+ |
DLGAP1 |
NNNAGAATGGGACACTACCC |
NGG |
2 |
0.5556 |
Tier II |
| 3 |
NC_000001.11 |
1 |
68138183 |
- |
WLS |
NNNAGGAAGAGACACTCCCC |
NGG |
2 |
0.1412 |
Tier II |
| 4 |
NC_000015.10 |
15 |
47330947 |
+ |
SEMA6D |
NNNAGGATGAGACACTATGC |
NGG |
2 |
0.0804 |
Tier II |
| 5 |
NC_000004.12 |
4 |
16858220 |
+ |
LDB2 |
NNNAGGATGAGTCACTTCCC |
NGG |
2 |
0.0444 |
Tier II |
| 6 |
NC_000017.11 |
17 |
58482325 |
- |
HSF5 |
NNNAGGATGAGACACTGCGC |
NGG |
2 |
0.0221 |
Tier II |
| 7 |
NC_000001.11 |
1 |
145629187 |
+ |
GPR89A |
NNNAGGATGAGACAGTTCCC |
NGG |
2 |
0.0067 |
Tier II |
| 8 |
NC_000001.11 |
1 |
147951819 |
+ |
GPR89B |
NNNAGGATGAGACAGTTCCC |
NGG |
2 |
0.0067 |
Tier II |
| 9 |
NC_000002.12 |
2 |
31641073 |
- |
SRD5A2 |
NNNAGGATGAGACAGTTCCC |
NGG |
2 |
0.0067 |
Tier II |
| 10 |
NC_000005.10 |
5 |
96266156 |
+ |
CAST |
NNNAGGATGAGACAGTTCCC |
NGG |
2 |
0.0067 |
Tier II |
| 11 |
NC_000006.12 |
6 |
89193837 |
+ |
GABRR1 |
NNNAGGATGAGACAGTTCCC |
NGG |
2 |
0.0067 |
Tier II |
| 12 |
NC_000015.10 |
15 |
67275400 |
- |
IQCH |
NNNAGGATGAGACAGTTCCC |
NGG |
2 |
0.0067 |
Tier II |
| 13 |
NC_000010.11 |
10 |
95125123 |
- |
LOC107984257 |
NNNAGGATGGGAAACTACCC |
NGG |
2 |
0.2137 |
Tier III |
| 14 |
NC_000001.11 |
1 |
68138183 |
- |
GNG12-AS1 |
NNNAGGAAGAGACACTCCCC |
NGG |
2 |
0.1412 |
Tier III |
| 15 |
NC_000021.9 |
21 |
34746067 |
- |
LINC01426 |
NNNACGATGAGACACTTCCC |
NGG |
2 |
0.1048 |
Tier III |
| 16 |
NC_000005.10 |
5 |
96266156 |
+ |
LOC101929710 |
NNNAGGATGAGACAGTTCCC |
NGG |
2 |
0.0067 |
Tier III |
| 17 |
NC_000015.10 |
15 |
56500485 |
+ |
LOC105370832 |
NNNAGGATGAGACAGTTCCC |
NGG |
2 |
0.0067 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
132734812 |
- |
Tbck |
NNNAGGATGAGAAACCACCC |
NGG |
2 |
0.2564 |
Tier I |
| 2 |
NC_000080.6 |
14 |
97998669 |
- |
Dach1 |
NNNAGGGTGAGACTCTACCC |
NGG |
2 |
0.3765 |
Tier II |
| 3 |
NC_000071.6 |
5 |
27383699 |
+ |
Dpp6 |
NNNAGGCAGAGACACTACCC |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000076.6 |
10 |
11174203 |
- |
Shprh |
NNNAGGAAGACACACTACCC |
NGG |
2 |
0.3429 |
Tier II |
| 5 |
NC_000068.7 |
2 |
156252285 |
- |
Phf20 |
NNNAGGATGAGACACCACAC |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000081.6 |
15 |
88926189 |
+ |
Ttll8 |
NNNAGGCTGAGACTCTACCC |
NGG |
2 |
0.2333 |
Tier II |
| 7 |
NC_000073.6 |
7 |
72202633 |
- |
Mctp2 |
NNNAGGATGAGAGACTACCC |
NGG |
1 |
0.1364 |
Tier II |
| 8 |
NC_000077.6 |
11 |
8449783 |
- |
Tns3 |
NNNAGGATGAGACACTGCTC |
NGG |
2 |
0.0814 |
Tier II |
| 9 |
NC_000067.6 |
1 |
160136903 |
- |
Tnn |
NNNAGGATGAGACACTTCAC |
NGG |
2 |
0.0571 |
Tier II |
| 10 |
NC_000070.6 |
4 |
56906735 |
- |
Tmem245 |
NNNAGGATGAGACACTACAC |
NGA |
2 |
0.0298 |
Tier II |
| 11 |
NC_000067.6 |
1 |
73706194 |
+ |
6030407O03Rik |
NNNAGGATGAGACACTGCCC |
NAG |
2 |
0.0458 |
Tier III |
Other clones with same target sequence:
(none)
