Construct: sgRNA BRDN0001149191
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGCCCTTCCCTCGCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TNK2 (10188)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75585
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
195872406 |
- |
TNK2 |
NNNGCCCTTCCCTCGCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
53256705 |
- |
IQSEC2 |
NNNGCCCTGCCCTCGCAACC |
NGG |
2 |
0.2653 |
Tier II |
| 3 |
NC_000007.14 |
7 |
6583664 |
+ |
ZDHHC4 |
NNNGCCCTTCCCTCGCCACG |
NGG |
1 |
0.1765 |
Tier II |
| 4 |
NC_000016.10 |
16 |
82627983 |
- |
CDH13 |
NNNGCCCTTCCCTCGCGCCG |
NGG |
2 |
0.0336 |
Tier II |
| 5 |
NC_000010.11 |
10 |
11280551 |
- |
CELF2 |
NNNGCCCTTCCCTGGCAACG |
NTG |
2 |
0.0 |
Tier II |
| 6 |
NC_000016.10 |
16 |
4651209 |
- |
MGRN1 |
NNNGCCCTTCTCTGGCAACG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000002.12 |
2 |
220232451 |
- |
LOC105373893 |
NNNGCCCTTCCCTCGAAACA |
NGG |
2 |
0.9375 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
14132233 |
+ |
Hivep2 |
NNNGCCCTTCCCTCCCAACA |
NGG |
2 |
0.2557 |
Tier I |
| 2 |
NC_000073.6 |
7 |
19065845 |
+ |
Rsph6a |
NNNGCCCTTCCCGGGCAACG |
NGG |
2 |
0.0 |
Tier I |
Other clones with same target sequence:
(none)
