Construct: sgRNA BRDN0001149192
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGACTTGGCATCATAACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STRADA (92335)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76056
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
63706729 |
+ |
STRADA |
NNNACTTGGCATCATAACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
167263991 |
- |
POU2F1 |
NNNACTTACCATCATAACCC |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000010.11 |
10 |
72741635 |
- |
MCU |
NNNACTTGGCAGCATAACAC |
NGG |
2 |
0.2143 |
Tier II |
| 4 |
NC_000018.10 |
18 |
57776163 |
+ |
ATP8B1 |
NNNACTTGGCATAATAAACC |
NGG |
2 |
0.2071 |
Tier II |
| 5 |
NC_000011.10 |
11 |
28452650 |
- |
METTL15 |
NNNACGTGGCATCATAACCC |
NAG |
2 |
0.1481 |
Tier II |
| 6 |
NC_000012.12 |
12 |
32310578 |
- |
BICD1 |
NNNACTTTGCATCCTAACCC |
NGG |
2 |
0.1339 |
Tier II |
| 7 |
NC_000008.11 |
8 |
36842566 |
+ |
KCNU1 |
NNNACTTGGAATCATAAGCC |
NGG |
2 |
0.1156 |
Tier II |
| 8 |
NC_000018.10 |
18 |
74895017 |
+ |
ZNF407 |
NNNACTTGACATCATAACCC |
NGA |
2 |
0.0446 |
Tier II |
| 9 |
NC_000001.11 |
1 |
154811164 |
+ |
KCNN3 |
NNNACTTGGAATCAGAACCC |
NGG |
2 |
0.0433 |
Tier II |
| 10 |
NC_000006.12 |
6 |
132505225 |
- |
STX7 |
NNNCCTTGGCATCAGAACCC |
NGG |
2 |
0.0176 |
Tier II |
| 11 |
NC_000001.11 |
1 |
167263991 |
- |
LOC124900412 |
NNNACTTACCATCATAACCC |
NGG |
2 |
0.5385 |
Tier III |
| 12 |
NC_000018.10 |
18 |
57776163 |
+ |
LOC124904310 |
NNNACTTGGCATAATAAACC |
NGG |
2 |
0.2071 |
Tier III |
| 13 |
NC_000015.10 |
15 |
70002220 |
- |
LOC101929129 |
NNNACTTTGCATCATAGCCC |
NGG |
2 |
0.1103 |
Tier III |
| 14 |
NC_000009.12 |
9 |
118929425 |
- |
LOC101928849 |
NNNACTTGCCATCATACCCC |
NGG |
2 |
0.095 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
44905710 |
+ |
Ap2a1 |
NNNTCTTGGCAGCATAACCC |
NGG |
2 |
0.3182 |
Tier I |
| 2 |
NC_000072.6 |
6 |
115792708 |
- |
Cand2 |
NNNACTTGGCATCACAAACC |
NGG |
2 |
0.1469 |
Tier I |
| 3 |
NC_000075.6 |
9 |
105313439 |
+ |
Nek11 |
NNNACTTGGCATCATAGCCT |
NGG |
2 |
0.0529 |
Tier I |
| 4 |
NC_000077.6 |
11 |
106167906 |
+ |
Strada |
NNNACTTGGCATCGTAACCC |
NGA |
2 |
0.0324 |
Tier I |
| 5 |
NC_000074.6 |
8 |
13048272 |
- |
F10 |
NNNACTTGCCATCATTACCC |
NGG |
2 |
0.0 |
Tier I |
| 6 |
NC_000075.6 |
9 |
31461020 |
+ |
Tmem45b |
NNNGCTTGGTATCATAACCC |
NGG |
2 |
0.5882 |
Tier II |
| 7 |
NC_000080.6 |
14 |
70312190 |
- |
Slc39a14 |
NNNACTTGGCACCATAAACC |
NGG |
2 |
0.5101 |
Tier II |
| 8 |
NC_000075.6 |
9 |
42796982 |
+ |
Grik4 |
NNNACTTGGGACCATAACCC |
NGG |
2 |
0.3684 |
Tier II |
| 9 |
NC_000072.6 |
6 |
121362443 |
+ |
Slc6a12 |
NNNACTTAGCATCACAACCC |
NGG |
2 |
0.2727 |
Tier II |
| 10 |
NC_000067.6 |
1 |
185321469 |
- |
Iars2 |
NNNACTGGGCATCATAACTC |
NGG |
2 |
0.2715 |
Tier II |
| 11 |
NC_000077.6 |
11 |
89656752 |
- |
Ankfn1 |
NNNACTTTGCATCATAACAC |
NGG |
2 |
0.2679 |
Tier II |
| 12 |
NC_000071.6 |
5 |
111134080 |
- |
Ttc28 |
NNNATTTGGCATCATAACCT |
NGG |
2 |
0.1909 |
Tier II |
| 13 |
NC_000069.6 |
3 |
89234635 |
- |
Trim46 |
NNNCCTTGGCATCATAACCA |
NGG |
2 |
0.1765 |
Tier II |
| 14 |
NC_000071.6 |
5 |
74005017 |
- |
Usp46 |
NNNACTTGGCATCATAGCCA |
NGG |
2 |
0.0882 |
Tier II |
| 15 |
NC_000071.6 |
5 |
24151645 |
- |
Klhl7 |
NNNACTTGGCATCAGAACCC |
NGT |
2 |
0.0008 |
Tier II |
| 16 |
NC_000083.6 |
17 |
90423045 |
+ |
Nrxn1 |
NNNACTTGGCATCAGAACCC |
NGT |
2 |
0.0008 |
Tier II |
| 17 |
NC_000084.6 |
18 |
42753240 |
- |
Ppp2r2b |
NNNACTTGGCACCATCACCC |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000073.6 |
7 |
109029501 |
- |
Tub |
NNNACTTGGCATCATAACCC |
NCC |
2 |
0.0 |
Tier II |
| 19 |
NC_000077.6 |
11 |
71548133 |
- |
9230020A06Rik |
NNNAGTTGGCATCATAACCT |
NGG |
2 |
0.18 |
Tier III |
| 20 |
NC_000067.6 |
1 |
76084323 |
+ |
Gm32622 |
NNNACTTGGCAGCCTAACCC |
NGG |
2 |
0.1071 |
Tier III |
| 21 |
NC_000070.6 |
4 |
137090152 |
+ |
Gm31648 |
NNNACTGGGCATCATCACCC |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
