Construct: sgRNA BRDN0001149193

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGACAATCCCAAACAAGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK10 (152110)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75634

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 27171820 - NEK10 NNNACAATCCCAAACAAGGT NGG 0 1.0 Tier I
2 NC_000012.12 12 104861328 + SLC41A2 NNNACAATCCCAACCAAGTT NGG 2 0.1429 Tier I
3 NC_000002.12 2 211386854 - ERBB4 NNNACAAACCCAAACAAGGT NTG 2 0.0312 Tier I
4 NC_000013.11 13 40636292 + FOXO1 NNNACAACCTCAAACAAGGT NGG 2 0.9412 Tier II
5 NC_000006.12 6 20578284 + CDKAL1 NNNACTACCCCAAACAAGGT NGG 2 0.7143 Tier II
6 NC_000007.14 7 44398386 + NUDCD3 NNNACAATCCCAGACAAGGT NGG 1 0.6522 Tier II
7 NC_000003.12 3 78917974 - ROBO1 NNNACAATCACAAACAATGT NGG 2 0.5778 Tier II
8 NC_000007.14 7 91952664 - AKAP9 NNNATAATACCAAACAAGGT NGG 2 0.5455 Tier II
9 NC_000019.10 19 40511470 - SPTBN4 NNNACAATGCAAAACAAGGT NGG 2 0.4643 Tier II
10 NC_000008.11 8 26773541 - ADRA1A NNNACATTCCCAGACAAGGT NGG 2 0.2853 Tier II
11 NC_000002.12 2 205285782 - PARD3B NNNAGCATCCCAAACAAGGT NGG 2 0.2727 Tier II
12 NC_000004.12 4 46952097 - GABRA4 NNNCCAATCCCAAACAATGT NGG 2 0.2353 Tier II
13 NC_000008.11 8 25460986 + CDCA2 NNNACAATCACAAACAGGGT NGG 2 0.1529 Tier II
14 NC_000003.12 3 39865504 + MYRIP NNNACAATCCCATAAAAGGT NGG 2 0.0667 Tier II
15 NC_000004.12 4 74237743 - MTHFD2L NNNACAGTCCCAAACAAGGT NTG 2 0.0275 Tier II
16 NC_000012.12 12 7654855 + APOBEC1 NNNCCAATCCCAAACAAGGT NGT 2 0.0057 Tier II
17 NC_000003.12 3 197454288 + LOC105374308 NNNACAAACTCAAACAAGGT NGG 2 0.7529 Tier III
18 NC_000006.12 6 84453771 - LINC01611 NNNACAATCCCAAACAAAGA NGG 2 0.3894 Tier III
19 NC_000006.12 6 84453771 - LOC107986620 NNNACAATCCCAAACAAAGA NGG 2 0.3894 Tier III
20 NC_000013.11 13 45700914 + LINC01055 NNNACAATCCTAGACAAGGT NGG 2 0.2007 Tier III
21 NC_000013.11 13 24954690 - TPTE2P1 NNNACAATTCCAAACAAGGG NGG 2 0.1544 Tier III
22 NC_000002.12 2 55951775 - LOC105374690 NNNACAATCCCAAATAAGGT NAG 2 0.0173 Tier III
23 NC_000002.12 2 55951775 - RN7SKP208 NNNACAATCCCAAATAAGGT NAG 2 0.0173 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 71370976 - Smchd1 NNNACTATCCCTAACAAGGT NGG 2 0.2381 Tier I
2 NC_000076.6 10 83281407 + Slc41a2 NNNACAATCCCAACCAAGTT NGG 2 0.1429 Tier I
3 NC_000067.6 1 52871885 + Hibch NNNACTATCTCAAACAAGGT NGG 2 0.6723 Tier II
4 NC_000069.6 3 146940518 - Ttll7 NNNACAGTACCAAACAAGGT NGG 2 0.605 Tier II
5 NC_000076.6 10 122732114 + Ppm1h NNNTCTATCCCAAACAAGGT NGG 2 0.4545 Tier II
6 NC_000074.6 8 105668603 - Ctcf NNNAAAATCCAAAACAAGGT NGG 2 0.4286 Tier II
7 NC_000069.6 3 139355572 - Stpg2 NNNTTAATCCCAAACAAGGT NGG 2 0.405 Tier II
8 NC_000068.7 2 121253224 + Trp53bp1 NNNACAATCCAAAGCAAGGT NGG 2 0.35 Tier II
9 NC_000070.6 4 134622050 + Man1c1 NNNCCAATCTCAAACAAGGT NGG 2 0.3322 Tier II
10 NC_000080.6 14 55559910 + Dcaf11 NNNACCATCCCAGACAAGGT NGG 2 0.2964 Tier II
11 NC_000069.6 3 116352705 + Cdc14a NNNAACATCCCAAACAAGGT NGG 2 0.2597 Tier II
12 NC_000072.6 6 83340955 - Mob1a NNNACAATCCCAAACAAGTT NAG 2 0.1728 Tier II
13 NC_000077.6 11 33346144 + Ranbp17 NNNAGAATCCCAAACAAGGT NAG 2 0.1556 Tier II
14 NC_000074.6 8 41373288 + Asah1 NNNACAATCCCAACCAAGTT NGG 2 0.1429 Tier II
15 NC_000074.6 8 122184596 - Zfp469 NNNACAATCCCAAACAAGAC NGG 2 0.0649 Tier II
16 NC_000084.6 18 32993664 - Camk4 NNNACATTCCCAAACAAGGT NGA 2 0.0304 Tier II
17 NC_000077.6 11 11971699 + Grb10 NNNACAACCCCAAACAAGGT NGT 2 0.0161 Tier II
18 NC_000073.6 7 57275125 + Gabrg3 NNNACAATCCCAAAGAAGGT NAG 2 0.013 Tier II
19 NC_000077.6 11 93222693 - Car10 NNNAAAATCCCAAACCAGGT NGG 2 0.0 Tier II
20 NC_000074.6 8 36197897 + Gm35520 NNNACAAACCCAAACAAGTT NGG 2 0.5333 Tier III
21 NC_000076.6 10 118745884 + LOC115485728 NNNTCAATCCCAAACAATGT NGG 2 0.4242 Tier III
22 NC_000078.6 12 113813278 + Igh NNNTCAATCCCAAACAATGT NGG 2 0.4242 Tier III
23 NC_000073.6 7 115028307 + Gm34225 NNNACAATCCTAAAGAAGGT NGG 2 0.0154 Tier III
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Other clones with same target sequence:

(none)