Construct: sgRNA BRDN0001149200

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AATGAGAACACCTCATTGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K5 (11183)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75711

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 50468657 + MAP4K5 NNNGAGAACACCTCATTGGG NGG 0 1.0 Tier I
2 NC_000003.12 3 63294308 - SYNPR NNNGATAACACCTCATTGGA NGG 2 0.625 Tier II
3 NC_000001.11 1 206716059 - MAPKAPK2 NNNAAGAACACCACATTGGG NGG 2 0.6231 Tier II
4 NC_000007.14 7 147923385 + CNTNAP2 NNNGAGAACACCACGTTGGG NGG 2 0.45 Tier II
5 NC_000007.14 7 32066824 + PDE1C NNNGACAACAGCTCATTGGG NGG 2 0.1705 Tier II
6 NC_000008.11 8 62877292 - NKAIN3 NNNGAGAACACCTTATTGGG NGA 2 0.0509 Tier II
7 NC_000009.12 9 38041612 + SHB NNNGAGAAAACCTCATTGGG NGT 2 0.0138 Tier II
8 NC_000001.11 1 145891393 - ITGA10 NNNGAGAAGACCTGATTGGG NGG 2 0.0 Tier II
9 NC_000002.12 2 187312307 + CALCRL-AS1 NNNGAGAACACATCATTTGG NGG 2 0.4762 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 114610393 - Dph6 NNNGAGAACACTTCATTGTG NGG 2 0.359 Tier II
2 NC_000077.6 11 121350583 + Wdr45b NNNGAGAACTCCTCAGTGGG NGG 2 0.3054 Tier II
3 NC_000075.6 9 58144424 - Stra6 NNNGAGAACACCACCTTGGG NGG 2 0.1888 Tier II
4 NC_000071.6 5 16851357 + Gm28710 NNNGAGAACACCTCAGAGGG NGG 2 0.1846 Tier II
5 NC_000075.6 9 60344677 - Thsd4 NNNGAGAACAGCTCATTGAG NGG 2 0.1786 Tier II
6 NC_000068.7 2 77607970 - Zfp385b NNNGAGAGCACCTCTTTGGG NGG 2 0.1467 Tier II
7 NC_000068.7 2 58194209 - Cytip NNNGACAACACCTCTTTGGG NGG 2 0.1364 Tier II
8 NC_000072.6 6 120995463 + Mical3 NNNGAGAACAGCTCCTTGGG NGG 2 0.0682 Tier II
9 NC_000072.6 6 71221061 + Smyd1 NNNGAGATCACCTCATGGGG NGG 2 0.0504 Tier II
10 NC_000072.6 6 120995463 + Gm43915 NNNGAGAACAGCTCCTTGGG NGG 2 0.0682 Tier III
Download CSV

Other clones with same target sequence:

(none)

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