Construct: sgRNA BRDN0001149200
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATGAGAACACCTCATTGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP4K5 (11183)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75711
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
50468657 |
+ |
MAP4K5 |
NNNGAGAACACCTCATTGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
63294308 |
- |
SYNPR |
NNNGATAACACCTCATTGGA |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000001.11 |
1 |
206716059 |
- |
MAPKAPK2 |
NNNAAGAACACCACATTGGG |
NGG |
2 |
0.6231 |
Tier II |
| 4 |
NC_000007.14 |
7 |
147923385 |
+ |
CNTNAP2 |
NNNGAGAACACCACGTTGGG |
NGG |
2 |
0.45 |
Tier II |
| 5 |
NC_000007.14 |
7 |
32066824 |
+ |
PDE1C |
NNNGACAACAGCTCATTGGG |
NGG |
2 |
0.1705 |
Tier II |
| 6 |
NC_000008.11 |
8 |
62877292 |
- |
NKAIN3 |
NNNGAGAACACCTTATTGGG |
NGA |
2 |
0.0509 |
Tier II |
| 7 |
NC_000009.12 |
9 |
38041612 |
+ |
SHB |
NNNGAGAAAACCTCATTGGG |
NGT |
2 |
0.0138 |
Tier II |
| 8 |
NC_000001.11 |
1 |
145891393 |
- |
ITGA10 |
NNNGAGAAGACCTGATTGGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000002.12 |
2 |
187312307 |
+ |
CALCRL-AS1 |
NNNGAGAACACATCATTTGG |
NGG |
2 |
0.4762 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
114610393 |
- |
Dph6 |
NNNGAGAACACTTCATTGTG |
NGG |
2 |
0.359 |
Tier II |
| 2 |
NC_000077.6 |
11 |
121350583 |
+ |
Wdr45b |
NNNGAGAACTCCTCAGTGGG |
NGG |
2 |
0.3054 |
Tier II |
| 3 |
NC_000075.6 |
9 |
58144424 |
- |
Stra6 |
NNNGAGAACACCACCTTGGG |
NGG |
2 |
0.1888 |
Tier II |
| 4 |
NC_000071.6 |
5 |
16851357 |
+ |
Gm28710 |
NNNGAGAACACCTCAGAGGG |
NGG |
2 |
0.1846 |
Tier II |
| 5 |
NC_000075.6 |
9 |
60344677 |
- |
Thsd4 |
NNNGAGAACAGCTCATTGAG |
NGG |
2 |
0.1786 |
Tier II |
| 6 |
NC_000068.7 |
2 |
77607970 |
- |
Zfp385b |
NNNGAGAGCACCTCTTTGGG |
NGG |
2 |
0.1467 |
Tier II |
| 7 |
NC_000068.7 |
2 |
58194209 |
- |
Cytip |
NNNGACAACACCTCTTTGGG |
NGG |
2 |
0.1364 |
Tier II |
| 8 |
NC_000072.6 |
6 |
120995463 |
+ |
Mical3 |
NNNGAGAACAGCTCCTTGGG |
NGG |
2 |
0.0682 |
Tier II |
| 9 |
NC_000072.6 |
6 |
71221061 |
+ |
Smyd1 |
NNNGAGATCACCTCATGGGG |
NGG |
2 |
0.0504 |
Tier II |
| 10 |
NC_000072.6 |
6 |
120995463 |
+ |
Gm43915 |
NNNGAGAACAGCTCCTTGGG |
NGG |
2 |
0.0682 |
Tier III |
Other clones with same target sequence:
(none)
