Construct: sgRNA BRDN0001149202
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATCAACCCATACTGCAGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AURKB (9212)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77617
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
8207180 |
+ |
AURKB |
NNNCAACCCATACTGCAGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
74218861 |
+ |
CCDC33 |
NNNCTCCCCATACTGCAGGT |
NGG |
2 |
0.1653 |
Tier I |
| 3 |
NC_000019.10 |
19 |
8910452 |
+ |
MUC16 |
NNNCTCCCCATACTGCAGGT |
NGG |
2 |
0.1653 |
Tier I |
| 4 |
NC_000004.12 |
4 |
148065748 |
- |
ARHGAP10 |
NNNCAAACCATACTGCAGGA |
NGG |
2 |
0.4219 |
Tier II |
| 5 |
NC_000017.11 |
17 |
64201655 |
- |
TEX2 |
NNNCAAGCCAGACTGCAGGT |
NGG |
2 |
0.1882 |
Tier II |
| 6 |
NC_000012.12 |
12 |
100517648 |
- |
NR1H4 |
NNNCAACCCTTACTGCTGGT |
NGG |
2 |
0.1176 |
Tier II |
| 7 |
NC_000001.11 |
1 |
44762852 |
+ |
KIF2C |
NNNCAACCCCTTCTGCAGGT |
NGG |
2 |
0.1111 |
Tier II |
| 8 |
NC_000009.12 |
9 |
130881685 |
+ |
ABL1 |
NNNCAACCCATATTGGAGGT |
NGG |
2 |
0.1077 |
Tier II |
| 9 |
NC_000008.11 |
8 |
95294192 |
+ |
CFAP418-AS1 |
NNNTAACCCTTACTGCAGGT |
NGG |
2 |
0.7059 |
Tier III |
| 10 |
NC_000013.11 |
13 |
63631154 |
+ |
LOC105370236 |
NNNCTACCCATATTGCAGGT |
NGG |
2 |
0.2545 |
Tier III |
| 11 |
NC_000005.10 |
5 |
172486461 |
+ |
LOC105377727 |
NNNCATCCCCTACTGCAGGT |
NGG |
2 |
0.2381 |
Tier III |
| 12 |
NC_000013.11 |
13 |
42866284 |
- |
LINC00428 |
NNNCCACCCAGACTGCAGGT |
NGG |
2 |
0.2 |
Tier III |
| 13 |
NC_000008.11 |
8 |
135435608 |
+ |
LOC101927872 |
NNNCAACCCATACTCCAGGA |
NGG |
2 |
0.1534 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
101992377 |
- |
Nhsl2 |
NNNAAACCCATACTGCAGAT |
NGG |
2 |
0.6015 |
Tier II |
| 2 |
NC_000077.6 |
11 |
6019011 |
- |
Camk2b |
NNNCAAGCCATACTGCATGT |
NGG |
2 |
0.3137 |
Tier II |
| 3 |
NC_000069.6 |
3 |
29192845 |
- |
Egfem1 |
NNNGAACCCATAATGCAGGT |
NGG |
2 |
0.1923 |
Tier II |
| 4 |
NC_000085.6 |
19 |
23234259 |
+ |
Smc5 |
NNNCAACCCACACTGCAGGT |
NAG |
2 |
0.1728 |
Tier II |
| 5 |
NC_000070.6 |
4 |
154038091 |
- |
Ccdc27 |
NNNCAACCCCTACTGTAGGT |
NGG |
2 |
0.1026 |
Tier II |
| 6 |
NC_000081.6 |
15 |
97748186 |
+ |
Rapgef3 |
NNNCAACCCAGACTGGAGGT |
NGG |
2 |
0.0615 |
Tier II |
Other clones with same target sequence:
(none)
