Construct: sgRNA BRDN0001149204
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGTACCTTTGATGTCCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKB (5257)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77846
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
47648607 |
- |
PHKB |
NNNGTACCTTTGATGTCCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
35869492 |
+ |
SRPK1 |
NNNGTACCTTTGTTGTCCAG |
NGG |
2 |
0.1286 |
Tier I |
| 3 |
NC_000019.10 |
19 |
49589895 |
+ |
PRRG2 |
NNNGTACCTTTGCTGTCCCC |
NGG |
2 |
0.0902 |
Tier I |
| 4 |
NC_000001.11 |
1 |
67791518 |
- |
GNG12 |
NNNGTACCATTGATGTCCCC |
NGG |
2 |
0.398 |
Tier II |
| 5 |
NC_000007.14 |
7 |
18087613 |
+ |
HDAC9 |
NNNGTACCTTTGATGTTTCG |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000002.12 |
2 |
63150138 |
- |
WDPCP |
NNNGTACCTTTGAAGTCCCC |
NGG |
2 |
0.2653 |
Tier II |
| 7 |
NC_000003.12 |
3 |
141368374 |
- |
ZBTB38 |
NNNGAACCTTTGATGTCCAG |
NGG |
2 |
0.2143 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
12235061 |
+ |
Map3k4 |
NNNGTACCTTTGATGTCTCG |
NTG |
2 |
0.025 |
Tier I |
| 2 |
NC_000067.6 |
1 |
43163333 |
- |
Fhl2 |
NNNGGACCTTTGATGTCCAG |
NGG |
2 |
0.2743 |
Tier II |
| 3 |
NC_000086.7 |
X |
12044933 |
- |
Bcor |
NNNGTTCCTTTGATGGCCCG |
NGG |
2 |
0.2473 |
Tier II |
| 4 |
NC_000071.6 |
5 |
34534345 |
+ |
Sh3bp2 |
NNNCTACCTTTGATGGCCCG |
NGG |
2 |
0.1833 |
Tier II |
| 5 |
NC_000074.6 |
8 |
83904974 |
- |
Adgrl1 |
NNNGTACCTTTGATGTCCCC |
NGA |
2 |
0.0298 |
Tier II |
| 6 |
NC_000074.6 |
8 |
105651924 |
- |
Ctcf |
NNNGTACTTTTGATGTCCCG |
NGT |
2 |
0.0141 |
Tier II |
| 7 |
NC_000075.6 |
9 |
96752810 |
+ |
E030011O05Rik |
NNNGAACCTTTGATGTCCAG |
NGG |
2 |
0.2143 |
Tier III |
| 8 |
NC_000078.6 |
12 |
32908914 |
+ |
Gm33484 |
NNNGTACCTTGGATGTACCG |
NGG |
2 |
0.1867 |
Tier III |
Other clones with same target sequence:
(none)
