Construct: sgRNA BRDN0001149205
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGACCAAAGCGGAAACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MATK (4145)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77013
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
3783163 |
- |
MATK |
NNNGACCAAAGCGGAAACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
197183710 |
- |
DLG1 |
NNNGCCCAAAGCAGAAACAC |
NGG |
2 |
0.4615 |
Tier I |
| 3 |
NC_000019.10 |
19 |
15355387 |
+ |
AKAP8 |
NNNGGCCAAAGAGGAAACAC |
NGG |
2 |
0.5143 |
Tier II |
| 4 |
NC_000001.11 |
1 |
2524025 |
- |
PANK4 |
NNNGCCCAAAGAGGAAACAC |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000003.12 |
3 |
29566496 |
- |
RBMS3 |
NNNGACCAAAGTGGAGACAC |
NGG |
2 |
0.1036 |
Tier II |
| 6 |
NC_000002.12 |
2 |
156524622 |
+ |
GPD2 |
NNNGACCAAAGCAGAAACAG |
NGG |
2 |
0.0543 |
Tier II |
| 7 |
NC_000019.10 |
19 |
15355387 |
+ |
LOC124904643 |
NNNGGCCAAAGAGGAAACAC |
NGG |
2 |
0.5143 |
Tier III |
| 8 |
NC_000017.11 |
17 |
78326186 |
+ |
LOC105371912 |
NNNGTCCAAAGCAGAAACAC |
NGG |
2 |
0.3357 |
Tier III |
| 9 |
NC_000008.11 |
8 |
133667046 |
- |
LINC03024 |
NNNGACCAAAGCTGGAACAC |
NGG |
2 |
0.195 |
Tier III |
| 10 |
NC_000005.10 |
5 |
125648954 |
+ |
LOC124901056 |
NNNGACAAAAGCGGAAACAC |
NGA |
2 |
0.0521 |
Tier III |
| 11 |
NC_000004.12 |
4 |
25031935 |
+ |
LOC124900684 |
NNNGACCAAAGCGGAAACAA |
NGT |
2 |
0.0081 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
75839820 |
- |
Zc3h3 |
NNNGACCAAAGAGCAAACAC |
NGG |
2 |
0.3061 |
Tier I |
| 2 |
NC_000080.6 |
14 |
69956620 |
+ |
Pebp4 |
NNNGACCAAAATGGAAACAC |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000082.6 |
16 |
32102179 |
- |
Cep19 |
NNNAACCAAAGTGGAAACAC |
NGG |
2 |
0.4846 |
Tier II |
| 4 |
NC_000082.6 |
16 |
87907475 |
+ |
Grik1 |
NNNGACCAAAGAGGAAACAA |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000075.6 |
9 |
21748123 |
- |
Ldlr |
NNNCAGCAAAGCGGAAACAC |
NGG |
2 |
0.2647 |
Tier II |
| 6 |
NC_000069.6 |
3 |
136817010 |
- |
Ppp3ca |
NNNTACCAAAGGGGAAACAC |
NGG |
2 |
0.1616 |
Tier II |
| 7 |
NC_000081.6 |
15 |
101229336 |
+ |
Grasp |
NNNGCCCAAAGCGGAAACCC |
NGG |
2 |
0.1034 |
Tier II |
| 8 |
NC_000070.6 |
4 |
151581939 |
+ |
Camta1 |
NNNGACCAAAGCGGAGCCAC |
NGG |
2 |
0.0339 |
Tier II |
| 9 |
NC_000075.6 |
9 |
67611495 |
- |
Tln2 |
NNNGACCAAAGAGGAAACAC |
NGT |
2 |
0.0115 |
Tier II |
| 10 |
NC_000084.6 |
18 |
20094702 |
+ |
Dsc1 |
NNNGACCAAAGCGGAAACAC |
NCA |
2 |
0.0 |
Tier II |
| 11 |
NC_000070.6 |
4 |
86433724 |
- |
Gm33730 |
NNNGACCAAAATGGAAACAC |
NGG |
2 |
0.5385 |
Tier III |
| 12 |
NC_000073.6 |
7 |
3111130 |
- |
Gm7353 |
NNNGACCAAAGAGCAAACAC |
NGG |
2 |
0.3061 |
Tier III |
| 13 |
NC_000085.6 |
19 |
32755510 |
- |
Gm36566 |
NNNGACCTAAGCTGAAACAC |
NGG |
2 |
0.1286 |
Tier III |
| 14 |
NC_000070.6 |
4 |
9124842 |
+ |
Gm50597 |
NNNGACCAAAGCGGGAACAG |
NGG |
2 |
0.0382 |
Tier III |
| 15 |
NC_000079.6 |
13 |
43314283 |
- |
Gm52047 |
NNNGACCAAAGGGGAAACAG |
NGG |
2 |
0.0261 |
Tier III |
Other clones with same target sequence:
(none)
