Construct: sgRNA BRDN0001149207
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCAATACTTAGGAACACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- VRK1 (7443)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75524
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
96847297 |
- |
VRK1 |
NNNAATACTTAGGAACACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
46176249 |
+ |
KANSL1 |
NNNAAAACTTAGGAAAACCC |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000012.12 |
12 |
130351466 |
- |
PIWIL1 |
NNNAATACTTAAGAACAACC |
NGG |
2 |
0.5026 |
Tier II |
| 4 |
NC_000023.11 |
X |
110140049 |
- |
TMEM164 |
NNNAATATTTAGCAACACCC |
NGG |
2 |
0.3684 |
Tier II |
| 5 |
NC_000004.12 |
4 |
38053258 |
- |
TBC1D1 |
NNNAACACTTAGTAACACCC |
NGG |
2 |
0.2727 |
Tier II |
| 6 |
NC_000011.10 |
11 |
131668339 |
- |
NTM |
NNNAATACTTAGGAATATCC |
NGG |
2 |
0.1978 |
Tier II |
| 7 |
NC_000023.11 |
X |
53109583 |
- |
KANTR |
NNNAATACTTAGGAATAACC |
NGG |
2 |
0.1657 |
Tier II |
| 8 |
NC_000005.10 |
5 |
59029132 |
+ |
PDE4D |
NNNAATACTTAGGAGCAGCC |
NGG |
2 |
0.0867 |
Tier II |
| 9 |
NC_000012.12 |
12 |
119045429 |
+ |
SRRM4 |
NNNAAGACTTAGGAACTCCC |
NGG |
2 |
0.0762 |
Tier II |
| 10 |
NC_000008.11 |
8 |
11108971 |
- |
XKR6 |
NNNAATACATAGGAACACCC |
NGA |
2 |
0.0645 |
Tier II |
| 11 |
NC_000003.12 |
3 |
188312784 |
+ |
LPP |
NNNAATACTTAAGAACACCG |
NGG |
2 |
0.0549 |
Tier II |
| 12 |
NC_000020.11 |
20 |
17450327 |
- |
PCSK2 |
NNNAATACTTAGGAAGAGCC |
NGG |
2 |
0.0205 |
Tier II |
| 13 |
NC_000012.12 |
12 |
119045429 |
+ |
LOC105370022 |
NNNAAGACTTAGGAACTCCC |
NGG |
2 |
0.0762 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
106051800 |
- |
Vrk1 |
NNNAATACTTAGGAACACCA |
NGG |
1 |
0.5 |
Tier I |
| 2 |
NC_000074.6 |
8 |
78713452 |
+ |
Slc10a7 |
NNNAAAACTTAGGAACACCC |
NGG |
1 |
0.8667 |
Tier II |
| 3 |
NC_000075.6 |
9 |
104252166 |
+ |
Dnajc13 |
NNNAAAACTCAGGAACACCC |
NGG |
2 |
0.4622 |
Tier II |
| 4 |
NC_000071.6 |
5 |
106647287 |
+ |
Zfp644 |
NNNAATACCTAGGGACACCC |
NGG |
2 |
0.4308 |
Tier II |
| 5 |
NC_000082.6 |
16 |
94791454 |
+ |
Kcnj6 |
NNNAATTCTTAGAAACACCC |
NGG |
2 |
0.4038 |
Tier II |
| 6 |
NC_000078.6 |
12 |
3773338 |
+ |
Dtnb |
NNNAAGACTGAGGAACACCC |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000078.6 |
12 |
38489893 |
- |
Dgkb |
NNNAAAACTTAGGAATACCC |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000067.6 |
1 |
170351045 |
+ |
Nos1ap |
NNNAATCCTTAGGGACACCC |
NGG |
2 |
0.2042 |
Tier II |
| 9 |
NC_000074.6 |
8 |
14079068 |
+ |
Erich1 |
NNNAATACCTAGGCACACCC |
NGG |
2 |
0.1978 |
Tier II |
| 10 |
NC_000086.7 |
X |
73119992 |
- |
Gm14685 |
NNNAATACTTGGGACCACCC |
NGG |
2 |
0.1773 |
Tier II |
| 11 |
NC_000086.7 |
X |
73146475 |
+ |
DXBay18 |
NNNAATACTTGGGACCACCC |
NGG |
2 |
0.1773 |
Tier II |
| 12 |
NC_000079.6 |
13 |
56634713 |
- |
Tgfbi |
NNNAATACTAAGGAACTCCC |
NGG |
2 |
0.1143 |
Tier II |
| 13 |
NC_000079.6 |
13 |
51755067 |
+ |
Sema4d |
NNNAATACTCAGGAAGACCC |
NGG |
2 |
0.0821 |
Tier II |
| 14 |
NC_000068.7 |
2 |
117151153 |
+ |
Spred1 |
NNNAATACTTAGGAACACCT |
NAG |
2 |
0.0778 |
Tier II |
| 15 |
NC_000071.6 |
5 |
23843092 |
+ |
Tomm7 |
NNNAATACTTAGGAACACCA |
NGA |
2 |
0.0347 |
Tier II |
Other clones with same target sequence:
(none)
