Construct: sgRNA BRDN0001149208
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGAAGGGAGATGTCATGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SIK2 (23235)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77138
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
111720624 |
- |
SIK2 |
NNNAAGGGAGATGTCATGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
51236788 |
- |
RNF11 |
NNNCAGGGAGATGTCATCCG |
NGG |
2 |
0.1681 |
Tier I |
| 3 |
NC_000011.10 |
11 |
111720624 |
- |
PPP2R1B |
NNNAAGGGAGATGTCATGCG |
NGG |
0 |
1.0 |
Tier II |
| 4 |
NC_000017.11 |
17 |
34986844 |
+ |
LIG3 |
NNNAAGGGAGATGTCATGCA |
NGG |
1 |
0.9375 |
Tier II |
| 5 |
NC_000013.11 |
13 |
20995138 |
+ |
LATS2 |
NNNAAAGGAGATGTCATGCT |
NGG |
2 |
0.7 |
Tier II |
| 6 |
NC_000009.12 |
9 |
127031526 |
- |
RALGPS1 |
NNNAAGGGAGGTGACATGCG |
NGG |
2 |
0.4024 |
Tier II |
| 7 |
NC_000003.12 |
3 |
108996018 |
+ |
MORC1 |
NNNAAGGGAGATGTCATATG |
NGG |
2 |
0.3195 |
Tier II |
| 8 |
NC_000004.12 |
4 |
16781284 |
+ |
LDB2 |
NNNAAGGCAGCTGTCATGCG |
NGG |
2 |
0.2462 |
Tier II |
| 9 |
NC_000011.10 |
11 |
66417467 |
+ |
NPAS4 |
NNNAAGGGAGAGGTCATGTG |
NGG |
2 |
0.2308 |
Tier II |
| 10 |
NC_000002.12 |
2 |
189930980 |
+ |
C2orf88 |
NNNCAGGGAGATGTCATCCG |
NGG |
2 |
0.1681 |
Tier II |
| 11 |
NC_000003.12 |
3 |
111999817 |
- |
TAGLN3 |
NNNAAGGGAGATGTAATGCT |
NGG |
2 |
0.1556 |
Tier II |
| 12 |
NC_000016.10 |
16 |
4212431 |
- |
SRL |
NNNAAGTGAGATGTCATGCG |
NTG |
2 |
0.0223 |
Tier II |
| 13 |
NC_000012.12 |
12 |
121874058 |
- |
HPD |
NNNAAGGGAGCTGTCATGCG |
NTG |
2 |
0.0156 |
Tier II |
| 14 |
NC_000008.11 |
8 |
76200895 |
+ |
LOC102724858 |
NNNAATGGAGATGTCATGCC |
NGG |
2 |
0.2857 |
Tier III |
| 15 |
NC_000002.12 |
2 |
189930980 |
+ |
RNF11P1 |
NNNCAGGGAGATGTCATCCG |
NGG |
2 |
0.1681 |
Tier III |
| 16 |
NC_000006.12 |
6 |
163342906 |
- |
DKFZp451B082 |
NNNAAGGGAGATGTCAGGTG |
NGG |
2 |
0.0543 |
Tier III |
| 17 |
NC_000012.12 |
12 |
121874058 |
- |
TIALD |
NNNAAGGGAGCTGTCATGCG |
NTG |
2 |
0.0156 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
50898621 |
+ |
Sik2 |
NNNAAAGGAGATGTCATGCG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000075.6 |
9 |
50898621 |
+ |
Ppp2r1b |
NNNAAAGGAGATGTCATGCG |
NGG |
1 |
1.0 |
Tier II |
| 3 |
NC_000080.6 |
14 |
67885854 |
- |
Dock5 |
NNNAAGAGAGATGTCATGCA |
NGG |
2 |
0.9375 |
Tier II |
| 4 |
NC_000080.6 |
14 |
34548834 |
+ |
Ldb3 |
NNNAAGGGAGAGGACATGCG |
NGG |
2 |
0.3095 |
Tier II |
| 5 |
NC_000077.6 |
11 |
88452795 |
- |
Msi2 |
NNNAAGGGAGATGCCATGAG |
NGG |
2 |
0.1224 |
Tier II |
| 6 |
NC_000076.6 |
10 |
73522373 |
+ |
Pcdh15 |
NNNAAGGGAGAAGTCATGGG |
NGG |
2 |
0.1 |
Tier II |
| 7 |
NC_000076.6 |
10 |
86139032 |
- |
Syn3 |
NNNAAGGGTGATGTCATGGG |
NGG |
2 |
0.075 |
Tier II |
| 8 |
NC_000076.6 |
10 |
105379935 |
+ |
Tmtc2 |
NNNAAGGGAGATGTCATGCT |
NGA |
2 |
0.0486 |
Tier II |
| 9 |
NC_000070.6 |
4 |
65888415 |
+ |
Astn2 |
NNNAAGGGAGATGTGATGAG |
NGG |
2 |
0.0214 |
Tier II |
| 10 |
NC_000076.6 |
10 |
106705457 |
+ |
Ppfia2 |
NNNAAGGGAGATGTGATGGG |
NGG |
2 |
0.0063 |
Tier II |
| 11 |
NC_000077.6 |
11 |
40211806 |
+ |
Gm12132 |
NNNAAGGGAGATGTCTTGCC |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
