Construct: sgRNA BRDN0001149214
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGGCTACACACAATTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GNE (10020)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77831
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
36246422 |
- |
GNE |
NNNGGCTACACACAATTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
103812935 |
- |
SH3PXD2A |
NNNGCATACACACAATTGTG |
NGG |
2 |
0.7296 |
Tier II |
| 3 |
NC_000010.11 |
10 |
106929749 |
+ |
SORCS1 |
NNNGACCACACACAATTGTG |
NGG |
2 |
0.5958 |
Tier II |
| 4 |
NC_000011.10 |
11 |
22220738 |
- |
ANO5 |
NNNGGCTACACACAACTTTG |
NGG |
2 |
0.4444 |
Tier II |
| 5 |
NC_000001.11 |
1 |
206595323 |
- |
EIF2D |
NNNGGCAACACAAAATTGTG |
NGG |
2 |
0.3365 |
Tier II |
| 6 |
NC_000020.11 |
20 |
53064708 |
- |
TSHZ2 |
NNNGGCTTCACACAATTGTG |
NGT |
2 |
0.0069 |
Tier II |
| 7 |
NC_000004.12 |
4 |
14222492 |
- |
LOC124900670 |
NNNTGCTATACACAATTGTG |
NGG |
2 |
0.3182 |
Tier III |
| 8 |
NC_000012.12 |
12 |
48204113 |
+ |
LOC102725258 |
NNNGGTTACACACAATTGGG |
NGG |
2 |
0.2321 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
105084460 |
- |
Nt5el |
NNNCACTACACACAATTGTG |
NGG |
2 |
0.4588 |
Tier II |
| 2 |
NC_000085.6 |
19 |
50457343 |
+ |
Sorcs1 |
NNNGGCCACACACAATTTTG |
NGG |
2 |
0.4583 |
Tier II |
| 3 |
NC_000068.7 |
2 |
21794089 |
- |
Gpr158 |
NNNGGTTCCACACAATTGTG |
NGG |
2 |
0.398 |
Tier II |
| 4 |
NC_000071.6 |
5 |
20375419 |
+ |
Magi2 |
NNNGGCTCCTCACAATTGTG |
NGG |
2 |
0.3782 |
Tier II |
| 5 |
NC_000074.6 |
8 |
83716596 |
+ |
Ddx39a |
NNNGGCTACACACAAGTTTG |
NGG |
2 |
0.2308 |
Tier II |
| 6 |
NC_000077.6 |
11 |
59687146 |
+ |
Mprip |
NNNGGCTTCACAAAATTGTG |
NGG |
2 |
0.1648 |
Tier II |
| 7 |
NC_000079.6 |
13 |
74360086 |
- |
Ccdc127 |
NNNGGCTACACAGAAATGTG |
NGG |
2 |
0.124 |
Tier II |
| 8 |
NC_000079.6 |
13 |
74360086 |
- |
Lrrc14b |
NNNGGCTACACAGAAATGTG |
NGG |
2 |
0.124 |
Tier II |
| 9 |
NC_000080.6 |
14 |
79598809 |
- |
Sugt1 |
NNNGGCTACACACAATTGTG |
NGC |
1 |
0.0222 |
Tier II |
| 10 |
NC_000075.6 |
9 |
69445282 |
+ |
Gm18587 |
NNNGGCTATAAACAATTGTG |
NGG |
2 |
0.6563 |
Tier III |
| 11 |
NC_000084.6 |
18 |
45698213 |
- |
A330093E20Rik |
NNNGTCTACACACAATAGTG |
NGG |
2 |
0.16 |
Tier III |
Other clones with same target sequence:
(none)
