Construct: sgRNA BRDN0001149215
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGCACCCAATTTGCATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK19 (23097)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76502
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
110667462 |
+ |
CDK19 |
NNNGCACCCAATTTGCATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
120762316 |
+ |
GLI2 |
NNNGCACCTAACTTGCATGG |
NGG |
2 |
0.8289 |
Tier II |
| 3 |
NC_000011.10 |
11 |
64217817 |
- |
FERMT3 |
NNNGCATCCAGTTTGCATGG |
NGG |
2 |
0.5281 |
Tier II |
| 4 |
NC_000009.12 |
9 |
126481942 |
- |
MVB12B |
NNNGCACCACATTTGCATGG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000011.10 |
11 |
64059069 |
+ |
FLRT1 |
NNNGCACCCAATTTGCACTG |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000011.10 |
11 |
64059069 |
+ |
MACROD1 |
NNNGCACCCAATTTGCACTG |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000003.12 |
3 |
17628407 |
+ |
TBC1D5 |
NNNACACCCAATTTGCATGG |
NAG |
2 |
0.2333 |
Tier II |
| 8 |
NC_000001.11 |
1 |
169592176 |
+ |
SELP |
NNNGCACACATTTTGCATGG |
NGG |
2 |
0.2 |
Tier II |
| 9 |
NC_000001.11 |
1 |
201705691 |
- |
NAV1 |
NNNGCACCCCATTTCCATGG |
NGG |
2 |
0.0909 |
Tier II |
| 10 |
NC_000008.11 |
8 |
10047126 |
+ |
MSRA-DT |
NNNGAACCCAATTTGCATAG |
NGG |
2 |
0.4082 |
Tier III |
| 11 |
NC_000001.11 |
1 |
201705691 |
- |
IPO9-AS1 |
NNNGCACCCCATTTCCATGG |
NGG |
2 |
0.0909 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
40436185 |
- |
Cdk19 |
NNNGCACCCAGTTTGCGTGG |
NGG |
2 |
0.1147 |
Tier I |
| 2 |
NC_000071.6 |
5 |
130080722 |
- |
Tpst1 |
NNNGCACTCAATTTGCATAG |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000068.7 |
2 |
28739258 |
- |
Ak8 |
NNNGCCCCCAATTTGAATGG |
NGG |
2 |
0.4545 |
Tier II |
| 4 |
NC_000078.6 |
12 |
111238588 |
+ |
Traf3 |
NNNGCACCCAATTTGCATGG |
NAG |
1 |
0.2593 |
Tier II |
| 5 |
NC_000073.6 |
7 |
78223269 |
+ |
Ntrk3 |
NNNCCACCCACTTTGCATGG |
NGG |
2 |
0.2118 |
Tier II |
| 6 |
NC_000076.6 |
10 |
52106526 |
- |
Ros1 |
NNNGCAACCAATGTGCATGG |
NGG |
2 |
0.1957 |
Tier II |
| 7 |
NC_000074.6 |
8 |
115082798 |
+ |
Wwox |
NNNGCACCCAATTTGCAGGG |
NAG |
2 |
0.0864 |
Tier II |
| 8 |
NC_000071.6 |
5 |
131974187 |
+ |
Auts2 |
NNNGCACCCAATTTACATGG |
NGA |
2 |
0.0654 |
Tier II |
| 9 |
NC_000086.7 |
X |
162434097 |
- |
Reps2 |
NNNGCACCCAATTTTCTTGG |
NGG |
2 |
0.019 |
Tier II |
| 10 |
NC_000073.6 |
7 |
7362774 |
- |
Gm3912 |
NNNGCACCCAATTTGCATGC |
NGT |
2 |
0.0069 |
Tier III |
| 11 |
NC_000073.6 |
7 |
8081222 |
- |
2810047C21Rik1 |
NNNGCACCCAATTTGCATGC |
NGT |
2 |
0.0069 |
Tier III |
Other clones with same target sequence:
(none)
