Construct: sgRNA BRDN0001149223
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAATGGAGTTTCTACACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MARK1 (4139)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77796
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
220579429 |
- |
MARK1 |
NNNATGGAGTTTCTACACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
103405139 |
- |
MARK3 |
NNNATAGAGTTTCTACACCG |
NGC |
2 |
0.0222 |
Tier I |
| 3 |
NC_000024.10 |
Y |
13328045 |
- |
UTY |
NNNATGGGGTTTCTACATCG |
NGG |
2 |
0.4714 |
Tier II |
| 4 |
NC_000012.12 |
12 |
45342247 |
- |
ANO6 |
NNNATGGAGTTTCTAAACTG |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000019.10 |
19 |
56597368 |
+ |
ZNF71 |
NNNATGAAGTTTCTACACCC |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000023.11 |
X |
112813220 |
- |
AMOT |
NNNATGGAGTATCTACACAG |
NGG |
2 |
0.3214 |
Tier II |
| 7 |
NC_000002.12 |
2 |
132476908 |
- |
GPR39 |
NNNATGGAGTGTCTACACAG |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000006.12 |
6 |
107038872 |
- |
MTRES1 |
NNNATGGGGTTTCTACACGG |
NGG |
2 |
0.0917 |
Tier II |
| 9 |
NC_000008.11 |
8 |
40702306 |
- |
ZMAT4 |
NNNATGGAGTTTCTACCCCG |
NGC |
2 |
0.0039 |
Tier II |
| 10 |
NC_000019.10 |
19 |
56597368 |
+ |
ZNF71-SMIM17 |
NNNATGAAGTTTCTACACCC |
NGG |
2 |
0.4286 |
Tier III |
| 11 |
NC_000008.11 |
8 |
67408741 |
+ |
ARFGEF1-DT |
NNNATGGAGTTTCTACACCA |
NGA |
2 |
0.0651 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
184944926 |
+ |
Mark1 |
NNNATGGAGTTTCTACACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000078.6 |
12 |
111592640 |
- |
Mark3 |
NNNATTGAGTTTCTACACCG |
NGC |
2 |
0.0148 |
Tier I |
| 3 |
NC_000071.6 |
5 |
74638701 |
- |
Lnx1 |
NNNATGGAGTGTCTACACCT |
NGG |
2 |
0.28 |
Tier II |
| 4 |
NC_000077.6 |
11 |
67569971 |
- |
Gas7 |
NNNATGGAGTTTCTACATCC |
NGG |
2 |
0.2755 |
Tier II |
| 5 |
NC_000069.6 |
3 |
87883005 |
+ |
Prcc |
NNNATGGAGTTTCTACACCA |
NAG |
2 |
0.2431 |
Tier II |
| 6 |
NC_000075.6 |
9 |
35107748 |
- |
St3gal4 |
NNNATGGAGTTTCTACCCAG |
NGG |
2 |
0.0756 |
Tier II |
| 7 |
NC_000072.6 |
6 |
99013482 |
- |
Foxp1 |
NNNATGGAGTTTCTCCCCCG |
NGG |
2 |
0.0481 |
Tier II |
| 8 |
NC_000071.6 |
5 |
141899579 |
+ |
Sdk1 |
NNNATGGAGTTTCTACACTG |
NGT |
2 |
0.0074 |
Tier II |
| 9 |
NC_000068.7 |
2 |
178498671 |
+ |
Gm35582 |
NNNATGGAGTTGCTACACTG |
NGG |
2 |
0.2308 |
Tier III |
Other clones with same target sequence:
(none)
