Construct: sgRNA BRDN0001149231
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTCTGCGTACTTCCAGACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EGFR (1956)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75690
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
55165377 |
- |
EGFR |
NNNTGCGTACTTCCAGACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
47877538 |
+ |
PKD1L1 |
NNNTGTGTGCTTCCAGACCA |
NGG |
2 |
0.619 |
Tier I |
| 3 |
NC_000002.12 |
2 |
206952580 |
+ |
CPO |
NNNTACGGACTTCCAGACCA |
NGG |
2 |
0.6356 |
Tier II |
| 4 |
NC_000016.10 |
16 |
4642923 |
- |
MGRN1 |
NNNTGCGTACTTGCAGTCCA |
NGG |
2 |
0.0182 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
16883553 |
- |
Egfr |
NNNTGCATACTTCCAGACCA |
NAG |
2 |
0.2593 |
Tier I |
| 2 |
NC_000071.6 |
5 |
135382998 |
+ |
Pom121 |
NNNTGTGTATTTCCAGACCA |
NGG |
2 |
0.8739 |
Tier II |
| 3 |
NC_000073.6 |
7 |
111764529 |
- |
Galnt18 |
NNNTGAGTTCTTCCAGACCA |
NGG |
2 |
0.5571 |
Tier II |
| 4 |
NC_000067.6 |
1 |
84285351 |
- |
Pid1 |
NNNTGGGTACTTCCAGACCT |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000067.6 |
1 |
185268270 |
+ |
Rab3gap2 |
NNNAGGGTACTTCCAGACCA |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000085.6 |
19 |
7537798 |
- |
Atl3 |
NNNTGGGTACTTCCAGTCCA |
NGG |
2 |
0.0667 |
Tier II |
| 7 |
NC_000072.6 |
6 |
33864814 |
+ |
Exoc4 |
NNNTGAGTACTTCCAGACCA |
NGA |
2 |
0.0645 |
Tier II |
| 8 |
NC_000086.7 |
X |
93972265 |
+ |
AU015836 |
NNNTCCGTGCTTCCAGACCA |
NGG |
2 |
0.5238 |
Tier III |
| 9 |
NC_000078.6 |
12 |
100775040 |
- |
Gm40558 |
NNNTGCGTACTTCCTGACCA |
NGG |
1 |
0.2 |
Tier III |
Other clones with same target sequence:
(none)
