Construct: sgRNA BRDN0001149233
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGATGAAGATGATCATCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRRK1 (79705)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75718
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
101022459 |
+ |
LRRK1 |
NNNATGAAGATGATCATCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
50332517 |
- |
CCNB3 |
NNNATGAGGAGGATCATCGT |
NGG |
2 |
0.2933 |
Tier II |
| 3 |
NC_000008.11 |
8 |
112966994 |
- |
CSMD3 |
NNNATGAAGATGATCATCAT |
NGA |
2 |
0.0496 |
Tier II |
| 4 |
NC_000002.12 |
2 |
132849063 |
+ |
NCKAP5 |
NNNATGCAGATGATCATCGT |
NGA |
2 |
0.0304 |
Tier II |
| 5 |
NC_000004.12 |
4 |
102939249 |
- |
SLC9B1 |
NNNATGAAGATGTTCATCGT |
NGT |
2 |
0.0048 |
Tier II |
| 6 |
NC_000017.11 |
17 |
80004643 |
- |
TBC1D16 |
NNNATGAAGATGTTCATCGT |
NGT |
2 |
0.0048 |
Tier II |
| 7 |
NC_000019.10 |
19 |
54370320 |
- |
LAIR1 |
NNNATGAAGATGATCATCGT |
NCT |
2 |
0.0 |
Tier II |
| 8 |
NC_000005.10 |
5 |
90969378 |
- |
ADGRV1 |
NNNATGAAGATGATCTTCTT |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000012.12 |
12 |
74270523 |
+ |
LINC02882 |
NNNATGAGGAAGATCATCGT |
NGG |
2 |
0.55 |
Tier III |
| 10 |
NC_000002.12 |
2 |
48282215 |
- |
LOC102724008 |
NNNATGAAGATGAACATCTT |
NGG |
2 |
0.4127 |
Tier III |
| 11 |
NC_000019.10 |
19 |
20512404 |
- |
LOC105372316 |
NNNATGAGGAGGATCATCGT |
NGG |
2 |
0.2933 |
Tier III |
| 12 |
NC_000014.9 |
14 |
54153783 |
+ |
LOC105370507 |
NNNATGAAGATGATCATTGT |
NGT |
2 |
0.0104 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
66294933 |
- |
Lrrk1 |
NNNATGAAGATGATCCTCGT |
NGG |
1 |
0.0 |
Tier I |
| 2 |
NC_000067.6 |
1 |
152544343 |
- |
Rgl1 |
NNNATGAAGGTGAGCATCGT |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000067.6 |
1 |
67183296 |
- |
Cps1 |
NNNATGAAAATGATCATCAT |
NGG |
2 |
0.4592 |
Tier II |
| 4 |
NC_000072.6 |
6 |
50445143 |
- |
Osbpl3 |
NNNGTGAAGATGAACATCGT |
NGG |
2 |
0.3869 |
Tier II |
| 5 |
NC_000075.6 |
9 |
122778562 |
- |
Topaz1 |
NNNATGAAAATGATCATCCT |
NGG |
2 |
0.2882 |
Tier II |
| 6 |
NC_000081.6 |
15 |
3989386 |
- |
AW549877 |
NNNATGAAGATTATCATCAT |
NGG |
2 |
0.2747 |
Tier II |
| 7 |
NC_000068.7 |
2 |
118831931 |
- |
Knstrn |
NNNATGAAGAGGATCATCCT |
NGG |
2 |
0.1793 |
Tier II |
| 8 |
NC_000076.6 |
10 |
85964067 |
+ |
Bpifc |
NNNATGAAGATGATAATTGT |
NGG |
2 |
0.1429 |
Tier II |
| 9 |
NC_000074.6 |
8 |
85525717 |
- |
Gpt2 |
NNNATGAAGATGAACAGCGT |
NGG |
2 |
0.0728 |
Tier II |
| 10 |
NC_000077.6 |
11 |
68184551 |
- |
Stx8 |
NNNAAGAAGATGATCATGGT |
NGG |
2 |
0.0667 |
Tier II |
| 11 |
NC_000068.7 |
2 |
156156994 |
- |
Rbm39 |
NNNATGATGATGATGATCGT |
NGG |
2 |
0.0214 |
Tier II |
| 12 |
NC_000077.6 |
11 |
20308828 |
+ |
Slc1a4 |
NNNATGAAGATGATCATCCT |
NTG |
2 |
0.0175 |
Tier II |
| 13 |
NC_000080.6 |
14 |
57821329 |
- |
Ska3 |
NNNATGAAGATGATCATTGT |
NGT |
2 |
0.0104 |
Tier II |
| 14 |
NC_000079.6 |
13 |
97116257 |
- |
Fam169a |
NNNATGAAGATGATCCCCGT |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000067.6 |
1 |
73525266 |
- |
C530043A13Rik |
NNNAGGAAGATGATCATCGT |
NGA |
2 |
0.0444 |
Tier III |
| 16 |
NC_000067.6 |
1 |
152544343 |
- |
Gm51749 |
NNNATGAAGGTGAGCATCGT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
