Construct: sgRNA BRDN0001149235
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAGTAGTTTAACTGATACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK1 (4750)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76126
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
169477480 |
- |
NEK1 |
NNNGTAGTTTAACTGATACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
32519815 |
- |
N4BP2L2 |
NNNCCAGTTTAACTGATACC |
NGG |
2 |
0.5294 |
Tier II |
| 3 |
NC_000020.11 |
20 |
13488463 |
- |
TASP1 |
NNNGTAGTTTAACTGATTCC |
NGG |
1 |
0.5 |
Tier II |
| 4 |
NC_000010.11 |
10 |
87956040 |
+ |
PTEN |
NNNGTAGTTTAATTGAAACC |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000001.11 |
1 |
148000736 |
- |
GPR89B |
NNNCTAGTTTAACAGATACC |
NGG |
2 |
0.3277 |
Tier II |
| 6 |
NC_000014.9 |
14 |
66601153 |
+ |
GPHN |
NNNGTAGTATAACTGATACC |
NAG |
2 |
0.2407 |
Tier II |
| 7 |
NC_000001.11 |
1 |
235244631 |
+ |
ARID4B |
NNNGTAGATTAACTGATACG |
NGG |
2 |
0.0471 |
Tier II |
| 8 |
NC_000007.14 |
7 |
147586986 |
+ |
CNTNAP2 |
NNNGTTGTTTAACTGATACC |
NTG |
2 |
0.0278 |
Tier II |
| 9 |
NC_000006.12 |
6 |
107224473 |
+ |
PDSS2 |
NNNGTAGCTTAACTGCTACC |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000017.11 |
17 |
19253151 |
- |
EPN2 |
NNNGTAGGTTAACGGATACC |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000002.12 |
2 |
213624106 |
- |
SPAG16 |
NNNGTAGTTTAACTTCTACC |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000001.11 |
1 |
60144034 |
+ |
LINC02778 |
NNNGTAATTTAACTGATAAC |
NGG |
2 |
0.4286 |
Tier III |
| 13 |
NC_000001.11 |
1 |
148000736 |
- |
PDZK1P1 |
NNNCTAGTTTAACAGATACC |
NGG |
2 |
0.3277 |
Tier III |
| 14 |
NC_000006.12 |
6 |
74173200 |
- |
LOC101928516 |
NNNGTAGTTTCAATGATACC |
NGG |
2 |
0.1538 |
Tier III |
| 15 |
NC_000015.10 |
15 |
88318769 |
+ |
LOC105370958 |
NNNGGAGTTTAACTGTTACC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
61089456 |
+ |
Nek1 |
NNNGAAGTTTAACTGATACC |
NAG |
2 |
0.1296 |
Tier I |
| 2 |
NC_000078.6 |
12 |
4215662 |
+ |
Cenpo |
NNNTTTGTTTAACTGATACC |
NGG |
2 |
0.2597 |
Tier II |
| 3 |
NC_000071.6 |
5 |
114734865 |
+ |
Git2 |
NNNGTAGTTTAACTGATGCA |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000080.6 |
14 |
61007783 |
+ |
Tnfrsf19 |
NNNGTAGTTGACCTGATACC |
NGG |
2 |
0.1316 |
Tier II |
| 5 |
NC_000077.6 |
11 |
18915431 |
- |
Meis1 |
NNNGTAGTTTAACTCATACT |
NGG |
2 |
0.0818 |
Tier II |
| 6 |
NC_000068.7 |
2 |
143973200 |
- |
Rrbp1 |
NNNGTAGTTTAACAGAGACC |
NGG |
2 |
0.0728 |
Tier II |
| 7 |
NC_000068.7 |
2 |
34585281 |
+ |
Mapkap1 |
NNNGTAGTTTAACTGCTATC |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000071.6 |
5 |
131290935 |
- |
Galnt17 |
NNNGTAGTTTAACTGTTTCC |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000079.6 |
13 |
52738277 |
+ |
BB123696 |
NNNGTAGTTGTACTGATACC |
NGG |
2 |
0.1538 |
Tier III |
| 10 |
NC_000085.6 |
19 |
4070596 |
+ |
Gm42060 |
NNNGTTGTTTAACTGAGACC |
NGG |
2 |
0.084 |
Tier III |
Other clones with same target sequence:
(none)
