Construct: sgRNA BRDN0001149240
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGAAATGTCAAACTAGCA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- OXSR1 (9943)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000003.12 | 3 | 38183099 | + | OXSR1 | NNNGAAATGTCAAACTAGCA | NGG | 0 | 1.0 | Tier I |
2 | NC_000022.11 | 22 | 28202632 | + | TTC28 | NNNAAGATGTCAAACTAGCA | NGG | 2 | 0.6429 | Tier II |
3 | NC_000001.11 | 1 | 213245714 | + | RPS6KC1 | NNNCAAATGCCAAACTAGCA | NGG | 2 | 0.2824 | Tier II |
4 | NC_000011.10 | 11 | 128846394 | + | KCNJ1 | NNNCAAATTTCAAACTAGCA | NGG | 2 | 0.2824 | Tier II |
5 | NC_000021.9 | 21 | 18060174 | + | CHODL | NNNAAAATGTCAAACTAGCC | NGG | 2 | 0.2045 | Tier II |
6 | NC_000012.12 | 12 | 113930015 | + | RBM19 | NNNGAAAGGTCAACCTAGCA | NGG | 2 | 0.1571 | Tier II |
7 | NC_000018.10 | 18 | 37157370 | - | KIAA1328 | NNNTAAATGTCAAACTAGAA | NGG | 2 | 0.1558 | Tier II |
8 | NC_000018.10 | 18 | 31925710 | + | TRAPPC8 | NNNGAAATTTCAAAATAGCA | NGG | 2 | 0.1185 | Tier II |
9 | NC_000017.11 | 17 | 39131148 | + | PLXDC1 | NNNGAAATGTGAAGCTAGCA | NGG | 2 | 0.1167 | Tier II |
10 | NC_000004.12 | 4 | 47253397 | + | GABRB1 | NNNGAAATGACAAACTAGGA | NGG | 2 | 0.1071 | Tier II |
11 | NC_000008.11 | 8 | 112233295 | - | CSMD3 | NNNGAAATGTCAGACTTGCA | NGG | 2 | 0.087 | Tier II |
12 | NC_000001.11 | 1 | 210639367 | - | HHAT | NNNGAAATGCCAAACTAGCA | NGA | 2 | 0.037 | Tier II |
13 | NC_000003.12 | 3 | 120409149 | + | FSTL1 | NNNGAAATGTCAAACTAGGC | NGG | 2 | 0.0284 | Tier II |
14 | NC_000001.11 | 1 | 39155307 | + | MACF1 | NNNGAAATGACAAACTAGCA | NGT | 2 | 0.0138 | Tier II |
15 | NC_000007.14 | 7 | 18113820 | - | HDAC9 | NNNGAAATGTCAAACTACCA | NGC | 2 | 0.0106 | Tier II |
16 | NC_000018.10 | 18 | 6058216 | + | L3MBTL4 | NNNGAAATGTCAAAATAGCA | NTG | 2 | 0.0087 | Tier II |
17 | NC_000004.12 | 4 | 172598510 | - | GALNTL6 | NNNGAAATGTCAAACTGGCA | NTG | 2 | 0.0069 | Tier II |
18 | NC_000001.11 | 1 | 119974483 | + | NOTCH2 | NNNGAAATGTCAAACTAGCA | NAT | 2 | 0.0 | Tier II |
19 | NC_000003.12 | 3 | 164535906 | + | LOC105374191 | NNNTAAATGTCAAACTAGAA | NGG | 2 | 0.1558 | Tier III |
20 | NC_000018.10 | 18 | 5924680 | + | LOC121725015 | NNNGAAATGTCAAACTGACA | NGG | 2 | 0.1222 | Tier III |
21 | NC_000019.10 | 19 | 28523401 | - | LOC100420587 | NNNGCAATGTCAAACTGGCA | NGG | 2 | 0.0882 | Tier III |
22 | NC_000011.10 | 11 | 90490594 | - | DISC1FP1 | NNNGAAATGTCAAAGTATCA | NGG | 2 | 0.0333 | Tier III |
23 | NC_000003.12 | 3 | 120409149 | + | BTNL12P | NNNGAAATGTCAAACTAGGC | NGG | 2 | 0.0284 | Tier III |
24 | NC_000004.12 | 4 | 138391512 | + | LINC00499 | NNNGAAATGACAAACTAGCA | NGT | 2 | 0.0138 | Tier III |
25 | NC_000002.12 | 2 | 221712394 | + | LOC105373899 | NNNGAAATGTCAACCTAGCA | NGC | 2 | 0.0048 | Tier III |
26 | NC_000001.11 | 1 | 59138133 | - | HSD52 | NNNGAAATGTCAAACTAGCA | NAT | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000073.6 | 7 | 98454720 | + | Gucy2d | NNNGAAAGGTCAAACAAGCA | NGG | 2 | 0.6667 | Tier II |
2 | NC_000075.6 | 9 | 42434191 | + | Tbcel | NNNGATATGTAAAACTAGCA | NGG | 2 | 0.5357 | Tier II |
3 | NC_000072.6 | 6 | 113674664 | - | Irak2 | NNNGAAATGACAAACTAGCT | NGG | 2 | 0.5143 | Tier II |
4 | NC_000072.6 | 6 | 38425328 | - | Ttc26 | NNNGAAATGTCAATCAAGCA | NGG | 2 | 0.4848 | Tier II |
5 | NC_000067.6 | 1 | 130140543 | + | Thsd7b | NNNGAAATGGCAAACAAGCA | NGG | 2 | 0.4545 | Tier II |
6 | NC_000080.6 | 14 | 47528649 | + | Fbxo34 | NNNCAAATGTAAAACTAGCA | NGG | 2 | 0.3971 | Tier II |
7 | NC_000084.6 | 18 | 64354502 | - | Onecut2 | NNNGAGATTTCAAACTAGCA | NGG | 2 | 0.381 | Tier II |
8 | NC_000067.6 | 1 | 139368654 | + | Crb1 | NNNGAAATGTCAAACTAGAG | NGG | 2 | 0.3277 | Tier II |
9 | NC_000075.6 | 9 | 54369365 | + | Dmxl2 | NNNGAGATGTCCAACTAGCA | NGG | 2 | 0.188 | Tier II |
10 | NC_000072.6 | 6 | 148943997 | + | Sinhcaf | NNNGAATTGTGAAACTAGCA | NGG | 2 | 0.1094 | Tier II |
11 | NC_000077.6 | 11 | 106190789 | + | Strada | NNNGAAATGTCAATCTAGGA | NGG | 2 | 0.0667 | Tier II |
12 | NC_000067.6 | 1 | 90921013 | - | Mlph | NNNGGAATGTCAAACTAGCA | NGA | 2 | 0.05 | Tier II |
13 | NC_000086.7 | X | 56963059 | + | Adgrg4 | NNNGAAATGTCAAACTAGTA | NCG | 2 | 0.0495 | Tier II |
14 | NC_000081.6 | 15 | 64361145 | + | Asap1 | NNNGAAATGTCAAAATAGGA | NGG | 2 | 0.0278 | Tier II |
15 | NC_000068.7 | 2 | 126634005 | + | Gabpb1 | NNNGAAAAGTCAAACTAGCA | NGC | 2 | 0.0178 | Tier II |
16 | NC_000083.6 | 17 | 28906585 | + | Bnip5 | NNNGAAATGTCAAACTAACA | NGC | 2 | 0.0154 | Tier II |
17 | NC_000068.7 | 2 | 142039859 | - | Macrod2 | NNNGAAATGACAAACTAGCA | NGT | 2 | 0.0138 | Tier II |
18 | NC_000082.6 | 16 | 70623643 | - | D16Ertd519e | NNNAAACTGTCAAACTAGCA | NGG | 2 | 0.3938 | Tier III |
19 | NC_000083.6 | 17 | 22015583 | - | Gm9620 | NNNGAATTGTGAAACTAGCA | NGG | 2 | 0.1094 | Tier III |
20 | NC_000083.6 | 17 | 28906585 | + | 1700030A11Rik | NNNGAAATGTCAAACTAACA | NGC | 2 | 0.0154 | Tier III |