Construct: sgRNA BRDN0001149240

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAGAAATGTCAAACTAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OXSR1 (9943)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77749

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 38183099 + OXSR1 NNNGAAATGTCAAACTAGCA NGG 0 1.0 Tier I
2 NC_000022.11 22 28202632 + TTC28 NNNAAGATGTCAAACTAGCA NGG 2 0.6429 Tier II
3 NC_000001.11 1 213245714 + RPS6KC1 NNNCAAATGCCAAACTAGCA NGG 2 0.2824 Tier II
4 NC_000011.10 11 128846394 + KCNJ1 NNNCAAATTTCAAACTAGCA NGG 2 0.2824 Tier II
5 NC_000021.9 21 18060174 + CHODL NNNAAAATGTCAAACTAGCC NGG 2 0.2045 Tier II
6 NC_000012.12 12 113930015 + RBM19 NNNGAAAGGTCAACCTAGCA NGG 2 0.1571 Tier II
7 NC_000018.10 18 37157370 - KIAA1328 NNNTAAATGTCAAACTAGAA NGG 2 0.1558 Tier II
8 NC_000018.10 18 31925710 + TRAPPC8 NNNGAAATTTCAAAATAGCA NGG 2 0.1185 Tier II
9 NC_000017.11 17 39131148 + PLXDC1 NNNGAAATGTGAAGCTAGCA NGG 2 0.1167 Tier II
10 NC_000004.12 4 47253397 + GABRB1 NNNGAAATGACAAACTAGGA NGG 2 0.1071 Tier II
11 NC_000008.11 8 112233295 - CSMD3 NNNGAAATGTCAGACTTGCA NGG 2 0.087 Tier II
12 NC_000001.11 1 210639367 - HHAT NNNGAAATGCCAAACTAGCA NGA 2 0.037 Tier II
13 NC_000003.12 3 120409149 + FSTL1 NNNGAAATGTCAAACTAGGC NGG 2 0.0284 Tier II
14 NC_000001.11 1 39155307 + MACF1 NNNGAAATGACAAACTAGCA NGT 2 0.0138 Tier II
15 NC_000007.14 7 18113820 - HDAC9 NNNGAAATGTCAAACTACCA NGC 2 0.0106 Tier II
16 NC_000018.10 18 6058216 + L3MBTL4 NNNGAAATGTCAAAATAGCA NTG 2 0.0087 Tier II
17 NC_000004.12 4 172598510 - GALNTL6 NNNGAAATGTCAAACTGGCA NTG 2 0.0069 Tier II
18 NC_000001.11 1 119974483 + NOTCH2 NNNGAAATGTCAAACTAGCA NAT 2 0.0 Tier II
19 NC_000003.12 3 164535906 + LOC105374191 NNNTAAATGTCAAACTAGAA NGG 2 0.1558 Tier III
20 NC_000018.10 18 5924680 + LOC121725015 NNNGAAATGTCAAACTGACA NGG 2 0.1222 Tier III
21 NC_000019.10 19 28523401 - LOC100420587 NNNGCAATGTCAAACTGGCA NGG 2 0.0882 Tier III
22 NC_000011.10 11 90490594 - DISC1FP1 NNNGAAATGTCAAAGTATCA NGG 2 0.0333 Tier III
23 NC_000003.12 3 120409149 + BTNL12P NNNGAAATGTCAAACTAGGC NGG 2 0.0284 Tier III
24 NC_000004.12 4 138391512 + LINC00499 NNNGAAATGACAAACTAGCA NGT 2 0.0138 Tier III
25 NC_000002.12 2 221712394 + LOC105373899 NNNGAAATGTCAACCTAGCA NGC 2 0.0048 Tier III
26 NC_000001.11 1 59138133 - HSD52 NNNGAAATGTCAAACTAGCA NAT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 98454720 + Gucy2d NNNGAAAGGTCAAACAAGCA NGG 2 0.6667 Tier II
2 NC_000075.6 9 42434191 + Tbcel NNNGATATGTAAAACTAGCA NGG 2 0.5357 Tier II
3 NC_000072.6 6 113674664 - Irak2 NNNGAAATGACAAACTAGCT NGG 2 0.5143 Tier II
4 NC_000072.6 6 38425328 - Ttc26 NNNGAAATGTCAATCAAGCA NGG 2 0.4848 Tier II
5 NC_000067.6 1 130140543 + Thsd7b NNNGAAATGGCAAACAAGCA NGG 2 0.4545 Tier II
6 NC_000080.6 14 47528649 + Fbxo34 NNNCAAATGTAAAACTAGCA NGG 2 0.3971 Tier II
7 NC_000084.6 18 64354502 - Onecut2 NNNGAGATTTCAAACTAGCA NGG 2 0.381 Tier II
8 NC_000067.6 1 139368654 + Crb1 NNNGAAATGTCAAACTAGAG NGG 2 0.3277 Tier II
9 NC_000075.6 9 54369365 + Dmxl2 NNNGAGATGTCCAACTAGCA NGG 2 0.188 Tier II
10 NC_000072.6 6 148943997 + Sinhcaf NNNGAATTGTGAAACTAGCA NGG 2 0.1094 Tier II
11 NC_000077.6 11 106190789 + Strada NNNGAAATGTCAATCTAGGA NGG 2 0.0667 Tier II
12 NC_000067.6 1 90921013 - Mlph NNNGGAATGTCAAACTAGCA NGA 2 0.05 Tier II
13 NC_000086.7 X 56963059 + Adgrg4 NNNGAAATGTCAAACTAGTA NCG 2 0.0495 Tier II
14 NC_000081.6 15 64361145 + Asap1 NNNGAAATGTCAAAATAGGA NGG 2 0.0278 Tier II
15 NC_000068.7 2 126634005 + Gabpb1 NNNGAAAAGTCAAACTAGCA NGC 2 0.0178 Tier II
16 NC_000083.6 17 28906585 + Bnip5 NNNGAAATGTCAAACTAACA NGC 2 0.0154 Tier II
17 NC_000068.7 2 142039859 - Macrod2 NNNGAAATGACAAACTAGCA NGT 2 0.0138 Tier II
18 NC_000082.6 16 70623643 - D16Ertd519e NNNAAACTGTCAAACTAGCA NGG 2 0.3938 Tier III
19 NC_000083.6 17 22015583 - Gm9620 NNNGAATTGTGAAACTAGCA NGG 2 0.1094 Tier III
20 NC_000083.6 17 28906585 + 1700030A11Rik NNNGAAATGTCAAACTAACA NGC 2 0.0154 Tier III
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Other clones with same target sequence:

(none)