GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001149242

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: ATTGGGAGCACAGATCGGAG

Original Target:

Taxon:: Homo sapiens (human)
Gene:: MATK (4145)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 77014

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000019.10	19	3781625	-	MATK	NNNGGGAGCACAGATCGGAG	NGG	0	1.0	Tier I
2	NC_000011.10	11	19441405	+	NAV2	NNNAGGAGAACAGATCGGAG	NGG	2	0.7714	Tier II
3	NC_000009.12	9	2621839	-	VLDLR	NNNGAGAGCACAGAACGGAG	NGG	2	0.5018	Tier II
4	NC_000017.11	17	75557497	-	LLGL2	NNNGGGAGCACAGATCAGAC	NGG	2	0.4	Tier II
5	NC_000023.11	X	134605089	-	PLAC1	NNNGGGAGCACTGATAGGAG	NGG	2	0.3333	Tier II
6	NC_000005.10	5	132556358	+	IL5	NNNGGGAGCACTGATCGGAA	NGG	2	0.3125	Tier II
7	NC_000001.11	1	12026694	-	MIIP	NNNGGAAGCACAGCTCGGAG	NGG	2	0.2143	Tier II
8	NC_000008.11	8	10303637	-	MSRA	NNNGGGACCACAGATTGGAG	NGG	2	0.1893	Tier II
9	NC_000004.12	4	98073978	-	STPG2	NNNGGGAGCAAAGATCTGAG	NGG	2	0.1875	Tier II
10	NC_000006.12	6	43782686	-	VEGFA	NNNGGGAGCCCAGGTCGGAG	NGG	2	0.1556	Tier II
11	NC_000006.12	6	43782686	-	POLR1C	NNNGGGAGCCCAGGTCGGAG	NGG	2	0.1556	Tier II
12	NC_000008.11	8	13268855	-	DLC1	NNNGGAAGCACAGATGGGAG	NGG	2	0.1538	Tier II
13	NC_000007.14	7	141590470	+	AGK	NNNGGGAGCACAGTCCGGAG	NGG	2	0.1455	Tier II
14	NC_000017.11	17	80359073	-	RNF213	NNNGGGAGCACAGATCCCAG	NGG	2	0.112	Tier II
15	NC_000007.14	7	152338258	-	KMT2C	NNNTGGAGCATAGATCGGAG	NGG	2	0.1119	Tier II
16	NC_000006.12	6	161551739	+	PRKN	NNNGGGAGCAGAGATCGGGG	NGG	2	0.0938	Tier II
17	NC_000008.11	8	38044915	+	EIF4EBP1	NNNGTGAGCACAGACCGGAG	NGG	2	0.0818	Tier II
18	NC_000019.10	19	57993873	+	ZNF606	NNNAGGAGCACAGAGCGGAG	NGG	2	0.045	Tier II
19	NC_000009.12	9	113582056	-	RGS3	NNNGGGAGCACAGATGTGAG	NGG	2	0.0385	Tier II
20	NC_000010.11	10	679737	-	DIP2C	NNNGGGAGCACAGATGGGCG	NGG	2	0.0318	Tier II
21	NC_000010.11	10	50374420	-	SGMS1	NNNGGGAGCACAGATTGGAG	NGA	2	0.0214	Tier II
22	NC_000015.10	15	78733182	-	LOC646934	NNNGGGAGCACAGATAGGAA	NGG	2	0.9375	Tier III
23	NC_000008.11	8	129631450	-	CCDC26	NNNAGGAGCACAGATAGGAG	NGG	2	0.9	Tier III
24	NC_000009.12	9	13920266	-	LOC101929507	NNNAGGAGCACAGATAGGAG	NGG	2	0.9	Tier III
25	NC_000009.12	9	2621839	-	VLDLR-AS1	NNNGAGAGCACAGAACGGAG	NGG	2	0.5018	Tier III
26	NC_000006.12	6	3908163	+	LOC107986558	NNNGGGAGCACAGTTCAGAG	NGG	2	0.4978	Tier III
27	NC_000017.11	17	80359073	-	RNF213-AS1	NNNGGGAGCACAGATCCCAG	NGG	2	0.112	Tier III
28	NC_000020.11	20	56167193	-	LOC105372680	NNNCGGAGCACAGATGGGAG	NGG	2	0.0814	Tier III
29	NC_000009.12	9	41367728	-	LOC107984035	NNNGGGAGCAGAGATTGGAG	NGG	2	0.0769	Tier III
30	NC_000009.12	9	41367728	-	LOC124900272	NNNGGGAGCAGAGATTGGAG	NGG	2	0.0769	Tier III
31	NC_000009.12	9	63695048	+	LOC124902167	NNNGGGAGCAGAGATTGGAG	NGG	2	0.0769	Tier III
32	NC_000021.9	21	10110698	+	LOC105372733	NNNGGGAGCAGAGATTGGAG	NGG	2	0.0769	Tier III
33	NT_187374.1	9	80797	+	LOC124905322	NNNGGGAGCAGAGATTGGAG	NGG	2	0.0769	Tier III
34	NC_000008.11	8	38566284	-	LOC105379384	NNNGGGAGCACAGAGCTGAG	NGG	2	0.0125	Tier III
35	NC_000023.11	X	80569983	-	HK2P1	NNNGGGAGCACAGAGGGGAG	NGG	2	0.0077	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000076.6	10	81260956	+	Matk	NNNGGGAGCACAGATTGGAG	NGG	1	0.3077	Tier I
2	NC_000084.6	18	61083094	-	Pdgfrb	NNNGGGAGCACAGCTCAGAG	NGG	2	0.2	Tier II
3	NC_000076.6	10	105495665	+	Tmtc2	NNNGGCAGCACAGACCGGAG	NGG	2	0.186	Tier II
4	NC_000075.6	9	48374812	+	Nxpe4	NNNGGGAGGACAGATGGGAG	NGG	2	0.0952	Tier II
5	NC_000073.6	7	137243686	+	Ebf3	NNNGGGAGCACAGCTCTGAG	NGG	2	0.0536	Tier II
6	NC_000068.7	2	33048148	-	Garnl3	NNNGGGAGCACAGAGCTGAG	NGG	2	0.0125	Tier II

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Other clones with same target sequence:

(none)