Construct: sgRNA BRDN0001149242

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTGGGAGCACAGATCGGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MATK (4145)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77014

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 3781625 - MATK NNNGGGAGCACAGATCGGAG NGG 0 1.0 Tier I
2 NC_000011.10 11 19441405 + NAV2 NNNAGGAGAACAGATCGGAG NGG 2 0.7714 Tier II
3 NC_000009.12 9 2621839 - VLDLR NNNGAGAGCACAGAACGGAG NGG 2 0.5018 Tier II
4 NC_000017.11 17 75557497 - LLGL2 NNNGGGAGCACAGATCAGAC NGG 2 0.4 Tier II
5 NC_000023.11 X 134605089 - PLAC1 NNNGGGAGCACTGATAGGAG NGG 2 0.3333 Tier II
6 NC_000005.10 5 132556358 + IL5 NNNGGGAGCACTGATCGGAA NGG 2 0.3125 Tier II
7 NC_000001.11 1 12026694 - MIIP NNNGGAAGCACAGCTCGGAG NGG 2 0.2143 Tier II
8 NC_000008.11 8 10303637 - MSRA NNNGGGACCACAGATTGGAG NGG 2 0.1893 Tier II
9 NC_000004.12 4 98073978 - STPG2 NNNGGGAGCAAAGATCTGAG NGG 2 0.1875 Tier II
10 NC_000006.12 6 43782686 - VEGFA NNNGGGAGCCCAGGTCGGAG NGG 2 0.1556 Tier II
11 NC_000008.11 8 13268855 - DLC1 NNNGGAAGCACAGATGGGAG NGG 2 0.1538 Tier II
12 NC_000007.14 7 141590470 + AGK NNNGGGAGCACAGTCCGGAG NGG 2 0.1455 Tier II
13 NC_000017.11 17 80359073 - RNF213 NNNGGGAGCACAGATCCCAG NGG 2 0.112 Tier II
14 NC_000007.14 7 152338258 - KMT2C NNNTGGAGCATAGATCGGAG NGG 2 0.1119 Tier II
15 NC_000006.12 6 161551739 + PRKN NNNGGGAGCAGAGATCGGGG NGG 2 0.0938 Tier II
16 NC_000008.11 8 38044915 + EIF4EBP1 NNNGTGAGCACAGACCGGAG NGG 2 0.0818 Tier II
17 NC_000019.10 19 57993873 + ZNF606 NNNAGGAGCACAGAGCGGAG NGG 2 0.045 Tier II
18 NC_000009.12 9 113582056 - RGS3 NNNGGGAGCACAGATGTGAG NGG 2 0.0385 Tier II
19 NC_000010.11 10 679737 - DIP2C NNNGGGAGCACAGATGGGCG NGG 2 0.0318 Tier II
20 NC_000010.11 10 50374420 - SGMS1 NNNGGGAGCACAGATTGGAG NGA 2 0.0214 Tier II
21 NC_000015.10 15 78733182 - LOC646934 NNNGGGAGCACAGATAGGAA NGG 2 0.9375 Tier III
22 NC_000008.11 8 129631450 - CCDC26 NNNAGGAGCACAGATAGGAG NGG 2 0.9 Tier III
23 NC_000009.12 9 13920266 - LOC101929507 NNNAGGAGCACAGATAGGAG NGG 2 0.9 Tier III
24 NC_000009.12 9 2621839 - VLDLR-AS1 NNNGAGAGCACAGAACGGAG NGG 2 0.5018 Tier III
25 NC_000006.12 6 3908163 + TGILR NNNGGGAGCACAGTTCAGAG NGG 2 0.4978 Tier III
26 NC_000017.11 17 80359073 - RNF213-AS1 NNNGGGAGCACAGATCCCAG NGG 2 0.112 Tier III
27 NC_000020.11 20 56167193 - LOC105372680 NNNCGGAGCACAGATGGGAG NGG 2 0.0814 Tier III
28 NC_000009.12 9 41367728 - LOC107984035 NNNGGGAGCAGAGATTGGAG NGG 2 0.0769 Tier III
29 NC_000009.12 9 41367728 - LOC124900272 NNNGGGAGCAGAGATTGGAG NGG 2 0.0769 Tier III
30 NC_000009.12 9 63695048 + LOC124902167 NNNGGGAGCAGAGATTGGAG NGG 2 0.0769 Tier III
31 NC_000021.9 21 10110698 + LOC105372733 NNNGGGAGCAGAGATTGGAG NGG 2 0.0769 Tier III
32 NT_187374.1 9 80797 + LOC124905322 NNNGGGAGCAGAGATTGGAG NGG 2 0.0769 Tier III
33 NC_000008.11 8 38566284 - LOC105379384 NNNGGGAGCACAGAGCTGAG NGG 2 0.0125 Tier III
34 NC_000023.11 X 80569983 - HK2P1 NNNGGGAGCACAGAGGGGAG NGG 2 0.0077 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 81260956 + Matk NNNGGGAGCACAGATTGGAG NGG 1 0.3077 Tier I
2 NC_000084.6 18 61083094 - Pdgfrb NNNGGGAGCACAGCTCAGAG NGG 2 0.2 Tier II
3 NC_000076.6 10 105495665 + Tmtc2 NNNGGCAGCACAGACCGGAG NGG 2 0.186 Tier II
4 NC_000075.6 9 48374812 + Nxpe4 NNNGGGAGGACAGATGGGAG NGG 2 0.0952 Tier II
5 NC_000073.6 7 137243686 + Ebf3 NNNGGGAGCACAGCTCTGAG NGG 2 0.0536 Tier II
6 NC_000068.7 2 33048148 - Garnl3 NNNGGGAGCACAGAGCTGAG NGG 2 0.0125 Tier II
Download CSV

Other clones with same target sequence:

(none)

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