Construct: sgRNA BRDN0001149243
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGAGTATGAGGATTCGGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- INSR (3643)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75508
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
7152843 |
- |
INSR |
NNNGAGTATGAGGATTCGGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
232486897 |
+ |
ECEL1 |
NNNGACTATGAGGGTTCGGC |
NGG |
2 |
0.3182 |
Tier II |
| 3 |
NC_000001.11 |
1 |
48240643 |
- |
SLC5A9 |
NNNGAGGTTGAGGATTCGGC |
NGG |
2 |
0.2521 |
Tier II |
| 4 |
NC_000015.10 |
15 |
40434718 |
+ |
IVD |
NNNGAGTATGAGGATTCTGG |
NGG |
2 |
0.0392 |
Tier II |
| 5 |
NC_000023.11 |
X |
30692266 |
- |
GK |
NNNGAGGATGAGGATTGGGC |
NGG |
2 |
0.0346 |
Tier II |
| 6 |
NC_000020.11 |
20 |
35452722 |
- |
GDF5 |
NNNGACTATGAGGAGTCGGC |
NGG |
2 |
0.0341 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
62293408 |
- |
Pard3b |
NNNGAGTATGAGGATGCAGC |
NGG |
2 |
0.2396 |
Tier II |
| 2 |
NC_000071.6 |
5 |
105656788 |
+ |
Gm32276 |
NNNGAGTATGAGGTTTTGGC |
NGG |
2 |
0.2489 |
Tier III |
| 3 |
NC_000081.6 |
15 |
98409568 |
- |
1700031M16Rik |
NNNGAGAATGAGGATTCGGC |
NGT |
2 |
0.0141 |
Tier III |
Other clones with same target sequence:
(none)
