Construct: sgRNA BRDN0001149244
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGATCGGCCAAATCACACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K15 (389840)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76100
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
19456969 |
+ |
MAP3K15 |
NNNTCGGCCAAATCACACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
123954214 |
+ |
SPAM1 |
NNNTCGGCCAAATCACTTGT |
NGG |
2 |
0.0857 |
Tier I |
| 3 |
NC_000001.11 |
1 |
27363498 |
+ |
MAP3K6 |
NNNTCGGCCACATCACAGGT |
NGG |
2 |
0.0533 |
Tier I |
| 4 |
NC_000012.12 |
12 |
71979786 |
+ |
TPH2 |
NNNTTGGCCTAATCACACGT |
NGG |
2 |
0.5615 |
Tier II |
| 5 |
NC_000002.12 |
2 |
229066830 |
- |
PID1 |
NNNTCGGCCAAATCACACTT |
NGT |
2 |
0.0108 |
Tier II |
| 6 |
NC_000017.11 |
17 |
10742021 |
+ |
TMEM220-AS1 |
NNNTCGCCCAAATCACAAGT |
NGG |
2 |
0.3702 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
160046668 |
- |
Map3k15 |
NNNTCAGCCAAATCACACGT |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
109973177 |
+ |
Lgr4 |
NNNTTGGCCAAATCTCACGT |
NGG |
2 |
0.1273 |
Tier II |
Other clones with same target sequence:
(none)
