Construct: sgRNA BRDN0001149250
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCACTTCCGAGTGGAGCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PKN1 (5585)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76275
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
14450384 |
+ |
PKN1 |
NNNCTTCCGAGTGGAGCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
56590973 |
+ |
MT3 |
NNNCCTCCGTGTGGAGCACG |
NGG |
2 |
0.8824 |
Tier II |
| 3 |
NC_000018.10 |
18 |
452857 |
+ |
COLEC12 |
NNNCTTCCAAGTGGAGCACA |
NGG |
2 |
0.6027 |
Tier II |
| 4 |
NC_000009.12 |
9 |
104991427 |
- |
CT70 |
NNNCTTCCGAGTGGAGCATG |
NGC |
2 |
0.0103 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
30902844 |
- |
Ptges |
NNNCTTGCGAGTGGAGCACA |
NGG |
2 |
0.4412 |
Tier II |
| 2 |
NC_000074.6 |
8 |
48126750 |
+ |
Dctd |
NNNCTTCAGAGTGGAGCAAG |
NGG |
2 |
0.2786 |
Tier II |
| 3 |
NC_000082.6 |
16 |
35705598 |
+ |
Slc49a4 |
NNNCTTCCGAGTGCTGCACG |
NGG |
2 |
0.0857 |
Tier II |
| 4 |
NC_000081.6 |
15 |
71713203 |
+ |
Fam135b |
NNNCTTCCGAGTGGAGGACA |
NGG |
2 |
0.0551 |
Tier II |
| 5 |
NC_000071.6 |
5 |
123523061 |
- |
Diablo |
NNNCTTCCGAGTGGAGCACG |
NTT |
2 |
0.0 |
Tier II |
| 6 |
NC_000077.6 |
11 |
120765693 |
- |
Gm11771 |
NNNCTTCAGAGTGGAGCACG |
NGG |
1 |
0.65 |
Tier III |
Other clones with same target sequence:
(none)
