Construct: sgRNA BRDN0001149251
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGCACACCATACCTCCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROS1 (6098)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75991
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
117403143 |
+ |
ROS1 |
NNNCACACCATACCTCCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
137871787 |
+ |
TNFAIP3 |
NNNAACACCATACCTCCATT |
NGG |
2 |
0.5895 |
Tier II |
| 3 |
NC_000004.12 |
4 |
72070534 |
- |
NPFFR2 |
NNNCACACGATACTTCCATG |
NGG |
2 |
0.454 |
Tier II |
| 4 |
NC_000011.10 |
11 |
10245259 |
- |
SBF2 |
NNNCACACCAGGCCTCCATG |
NGG |
2 |
0.2889 |
Tier II |
| 5 |
NC_000022.11 |
22 |
35432312 |
- |
MCM5 |
NNNCACACCAGGCCTCCATG |
NGG |
2 |
0.2889 |
Tier II |
| 6 |
NC_000010.11 |
10 |
12535873 |
- |
CAMK1D |
NNNCACACCACAACTCCATG |
NGG |
2 |
0.2564 |
Tier II |
| 7 |
NC_000001.11 |
1 |
53020753 |
+ |
SCP2 |
NNNCCCACCATACCTCTATG |
NGG |
2 |
0.2333 |
Tier II |
| 8 |
NC_000011.10 |
11 |
43961246 |
- |
ACCSL |
NNNCACACCATATCTTCATG |
NGG |
2 |
0.2154 |
Tier II |
| 9 |
NC_000007.14 |
7 |
143323624 |
- |
CLCN1 |
NNNCAGACCAGACCTCCATG |
NGG |
2 |
0.2 |
Tier II |
| 10 |
NC_000003.12 |
3 |
65538394 |
+ |
MAGI1 |
NNNCACACCATCCTTCCATG |
NGG |
2 |
0.193 |
Tier II |
| 11 |
NC_000003.12 |
3 |
179379057 |
+ |
MFN1 |
NNNCACACCAGACCTCAATG |
NGG |
2 |
0.1867 |
Tier II |
| 12 |
NC_000001.11 |
1 |
66552000 |
+ |
SGIP1 |
NNNCTCACCATACCTCTATG |
NGG |
2 |
0.1697 |
Tier II |
| 13 |
NC_000018.10 |
18 |
47139079 |
+ |
HDHD2 |
NNNCACACCCTAACTCCATG |
NGG |
2 |
0.1282 |
Tier II |
| 14 |
NC_000007.14 |
7 |
95846732 |
+ |
DYNC1I1 |
NNNCTCACCATTCCTCCATG |
NGG |
2 |
0.1212 |
Tier II |
| 15 |
NC_000001.11 |
1 |
3169179 |
- |
PRDM16 |
NNNCACACAATACCTCCATG |
NGC |
2 |
0.019 |
Tier II |
| 16 |
NC_000006.12 |
6 |
35866971 |
- |
SRPK1 |
NNNCACACTATACCTCCATG |
NGT |
2 |
0.0141 |
Tier II |
| 17 |
NC_000018.10 |
18 |
27588137 |
+ |
LOC107985126 |
NNNAACACCATACTTCCATG |
NGG |
2 |
0.6175 |
Tier III |
| 18 |
NC_000010.11 |
10 |
132995318 |
+ |
LOC107984283 |
NNNCACACCTGACCTCCATG |
NGG |
2 |
0.3529 |
Tier III |
| 19 |
NC_000010.11 |
10 |
132995381 |
+ |
LOC107984283 |
NNNCACACCTGACCTCCATG |
NGG |
2 |
0.3529 |
Tier III |
| 20 |
NC_000010.11 |
10 |
132995444 |
+ |
LOC107984283 |
NNNCACACCTGACCTCCATG |
NGG |
2 |
0.3529 |
Tier III |
| 21 |
NC_000002.12 |
2 |
220073084 |
- |
LOC105373893 |
NNNCACACCATACCACCCTG |
NGG |
2 |
0.1103 |
Tier III |
| 22 |
NC_000020.11 |
20 |
1330559 |
+ |
SDCBP2-AS1 |
NNNCACACCATTCCTCCAGG |
NGG |
2 |
0.0833 |
Tier III |
| 23 |
NC_000020.11 |
20 |
1330559 |
+ |
FKBP1A-SDCBP2 |
NNNCACACCATTCCTCCAGG |
NGG |
2 |
0.0833 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
82640372 |
+ |
Saxo2 |
NNNCACACCATATCTCCATG |
NGG |
1 |
0.7 |
Tier II |
| 2 |
NC_000074.6 |
8 |
99061195 |
- |
Cdh8 |
NNNCACACCATGTCTCCATG |
NGG |
2 |
0.5056 |
Tier II |
| 3 |
NC_000071.6 |
5 |
112981669 |
- |
Grk3 |
NNNCACAGCACACCTCCATG |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000079.6 |
13 |
18798572 |
- |
Vps41 |
NNNCACATCATACCTCCATC |
NGG |
2 |
0.375 |
Tier II |
| 5 |
NC_000073.6 |
7 |
111581690 |
- |
Galnt18 |
NNNCTCACCTTACCTCCATG |
NGG |
2 |
0.3209 |
Tier II |
| 6 |
NC_000078.6 |
12 |
82979749 |
+ |
Rgs6 |
NNNCACCCCATGCCTCCATG |
NGG |
2 |
0.316 |
Tier II |
| 7 |
NC_000080.6 |
14 |
23987992 |
- |
Kcnma1 |
NNNCACACCATTTCTCCATG |
NGG |
2 |
0.2333 |
Tier II |
| 8 |
NC_000080.6 |
14 |
115203064 |
+ |
Gpc5 |
NNNCACACCCTATCTCCATG |
NGG |
2 |
0.2333 |
Tier II |
| 9 |
NC_000076.6 |
10 |
87205798 |
- |
1700113H08Rik |
NNNCCCACCATACCTCCATC |
NGG |
2 |
0.2143 |
Tier II |
| 10 |
NC_000082.6 |
16 |
13948953 |
- |
Mpv17l |
NNNCACACCATCTCTCCATG |
NGG |
2 |
0.1842 |
Tier II |
| 11 |
NC_000076.6 |
10 |
28701206 |
+ |
Themis |
NNNCAGACCATACCTTCATG |
NGG |
2 |
0.1538 |
Tier II |
| 12 |
NC_000077.6 |
11 |
110432872 |
- |
Map2k6 |
NNNCACACCATACACCCATG |
NGG |
2 |
0.0955 |
Tier II |
| 13 |
NC_000076.6 |
10 |
24181893 |
+ |
Stx7 |
NNNCACACCATCCCTCCACG |
NGG |
2 |
0.0726 |
Tier II |
| 14 |
NC_000082.6 |
16 |
14435032 |
- |
Abcc1 |
NNNCACACCATCCCTCCATG |
NAG |
2 |
0.0682 |
Tier II |
| 15 |
NC_000072.6 |
6 |
144190537 |
- |
Sox5 |
NNNCACACCATACCTCAATG |
NGT |
2 |
0.0075 |
Tier II |
| 16 |
NC_000074.6 |
8 |
23249506 |
- |
Golga7 |
NNNCACACCATTCCTCCATG |
NGT |
2 |
0.0054 |
Tier II |
| 17 |
NC_000082.6 |
16 |
84737379 |
- |
Gm26153 |
NNNCACACCATATCTCCATG |
NGG |
1 |
0.7 |
Tier III |
| 18 |
NC_000069.6 |
3 |
122297070 |
- |
Gm40190 |
NNNCACCCCATACCTCCATG |
NGG |
1 |
0.4375 |
Tier III |
| 19 |
NC_000067.6 |
1 |
59358211 |
+ |
Gm51625 |
NNNCACACCAGACCTACATG |
NGG |
2 |
0.4 |
Tier III |
| 20 |
NC_000075.6 |
9 |
63778669 |
- |
Gm35941 |
NNNCAAACCATTCCTCCATG |
NGG |
2 |
0.3095 |
Tier III |
| 21 |
NC_000086.7 |
X |
7547442 |
+ |
Gm23082 |
NNNCACACCAGGCCTCCATG |
NGG |
2 |
0.2889 |
Tier III |
| 22 |
NC_000074.6 |
8 |
23249506 |
- |
Gm39141 |
NNNCACACCATTCCTCCATG |
NGT |
2 |
0.0054 |
Tier III |
Other clones with same target sequence:
(none)
