Construct: sgRNA BRDN0001149255

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTCTTTGTAGACGGGAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDXK (8566)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76936

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000021.9 21 43749015 - PDXK NNNCTTTGTAGACGGGAAGG NGG 0 1.0 Tier I
2 NC_000011.10 11 133934122 + IGSF9B NNNCTTTGAAGACAGGAAGG NGG 2 0.6964 Tier II
3 NC_000020.11 20 5953164 - MCM8 NNNCTTTAGAGACGGGAAGG NGG 2 0.619 Tier II
4 NC_000003.12 3 10523233 + ATP2B2 NNNTTTTGTAGATGGGAAGG NGG 2 0.56 Tier II
5 NC_000015.10 15 99109482 + SYNM NNNTTTTGTAGATGGGAAGG NGG 2 0.56 Tier II
6 NC_000011.10 11 847728 + TSPAN4 NNNCTTTGTAGATGGGAAAG NGG 2 0.5 Tier II
7 NC_000012.12 12 118983371 + SRRM4 NNNGTTTGCAGACGGGAAGG NGG 2 0.4615 Tier II
8 NC_000011.10 11 66627059 + RBM14 NNNCTTTTTAGATGGGAAGG NGG 2 0.4375 Tier II
9 NC_000011.10 11 66627059 + RBM14-RBM4 NNNCTTTTTAGATGGGAAGG NGG 2 0.4375 Tier II
10 NC_000009.12 9 2085979 + SMARCA2 NNNCTGTGTAGACAGGAAGG NGG 2 0.4286 Tier II
11 NC_000016.10 16 1505224 - TELO2 NNNCTTGGTAGGCGGGAAGG NGG 2 0.4248 Tier II
12 NC_000007.14 7 5494632 + FBXL18 NNNCTTTGCAGACCGGAAGG NGG 2 0.3956 Tier II
13 NC_000007.14 7 117278130 + WNT2 NNNCCTTGTAGAAGGGAAGG NGG 2 0.3846 Tier II
14 NC_000018.10 18 37364858 + CELF4 NNNCTCTGTAGACTGGAAGG NGG 2 0.2424 Tier II
15 NC_000010.11 10 72111084 - ASCC1 NNNCTTTGCAGAGGGGAAGG NGG 2 0.1259 Tier II
16 NC_000010.11 10 11582990 - USP6NL NNNCTTTGTAGAATGGAAGG NGG 2 0.1026 Tier II
17 NC_000006.12 6 42395187 - TRERF1 NNNCTTTGTAGAGGGGAAGT NGG 2 0.0955 Tier II
18 NC_000006.12 6 34041771 + GRM4 NNNCTTTGTGGAGGGGAAGG NGG 2 0.0758 Tier II
19 NC_000020.11 20 15004885 + MACROD2 NNNCTTTGTAGACTTGAAGG NGG 2 0.0381 Tier II
20 NC_000014.9 14 103668145 + KLC1 NNNCTTTGTAGATGGGAAGG NTG 2 0.0273 Tier II
21 NC_000012.12 12 121549161 + KDM2B NNNCTTTGTAGAAGGGAAGG NGA 2 0.0267 Tier II
22 NC_000015.10 15 78174161 + ACSBG1 NNNCTTTGTAGACGGGAAAG NGC 2 0.0159 Tier II
23 NC_000004.12 4 167023924 - SPOCK3 NNNCTTTGTAGAGGGCAAGG NGG 2 0.0 Tier II
24 NC_000010.11 10 123391161 + LINC02641 NNNCTTCGTAGAAGGGAAGG NGG 2 0.2644 Tier III
25 NC_000009.12 9 40491632 + AQP7P5 NNNCTTTGCAGACGGGCAGG NGG 2 0.1629 Tier III
26 NC_000009.12 9 40491632 + LOC124902160 NNNCTTTGCAGACGGGCAGG NGG 2 0.1629 Tier III
27 NC_000009.12 9 43119962 + LOC102724922 NNNCTTTGCAGACGGGCAGG NGG 2 0.1629 Tier III
28 NC_000009.12 9 63133323 - LOC105379814 NNNCTTTGCAGACGGGCAGG NGG 2 0.1629 Tier III
29 NC_000009.12 9 63327135 + AQP7P1 NNNCTTTGCAGACGGGCAGG NGG 2 0.1629 Tier III
30 NC_000004.12 4 22693746 - GBA3 NNNCTTTGTGGAGGGGAAGG NGG 2 0.0758 Tier III
31 NC_000001.11 1 152945114 - LINC01527 NNNCTTTGTAGATGGGAAGG NGC 2 0.0156 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 5039875 + Ppp1r9a NNNCAATGTAGACGGGAAGG NGG 2 0.4333 Tier II
2 NC_000076.6 10 25180482 - Akap7 NNNGTTTGTAGACAGGAAGG NGG 2 0.375 Tier II
3 NC_000068.7 2 168636981 + Atp9a NNNCTTTGTACCCGGGAAGG NGG 2 0.1128 Tier II
4 NC_000086.7 X 7862360 + Otud5 NNNCTTTGTAGGGGGGAAGG NGG 2 0.0985 Tier II
5 NC_000085.6 19 31558654 + Prkg1 NNNCTTTGTAGAAGGGAAGG NTG 2 0.015 Tier II
6 NC_000075.6 9 58837953 - Hcn4 NNNCTTTCTAGACGGGAAGG NGC 2 0.0137 Tier II
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Other clones with same target sequence:

(none)