Construct: sgRNA BRDN0001149258
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACTATCACCAACATAATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITK (3702)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77447
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
157240188 |
+ |
ITK |
NNNTATCACCAACATAATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
106258468 |
- |
NAMPT |
NNNTATCACTAACATAATGT |
NGG |
2 |
0.6588 |
Tier II |
| 3 |
NC_000001.11 |
1 |
63470562 |
- |
ITGB3BP |
NNNTATCACAAACATAATGT |
NGG |
2 |
0.6067 |
Tier II |
| 4 |
NC_000003.12 |
3 |
2176593 |
+ |
CNTN4 |
NNNTATGGCCAACATAATGG |
NGG |
2 |
0.3451 |
Tier II |
| 5 |
NC_000006.12 |
6 |
56828393 |
+ |
DST |
NNNTATCACCAACATAATGC |
NAG |
2 |
0.1111 |
Tier II |
| 6 |
NC_000020.11 |
20 |
8543024 |
- |
PLCB1 |
NNNTATCACCAACATAAGGG |
NAG |
2 |
0.0864 |
Tier II |
| 7 |
NC_000009.12 |
9 |
17982860 |
+ |
ADAMTSL1 |
NNNTATCACCAAGATAATGG |
NAG |
2 |
0.0354 |
Tier II |
| 8 |
NC_000006.12 |
6 |
83570477 |
+ |
SNAP91 |
NNNTATCACCAACACAATGG |
NGA |
2 |
0.0189 |
Tier II |
| 9 |
NC_000004.12 |
4 |
145497507 |
- |
SMAD1 |
NNNTATCACCAACATAAAGG |
NGC |
2 |
0.0148 |
Tier II |
| 10 |
NC_000002.12 |
2 |
122215483 |
+ |
LOC105373592 |
NNNCATCAGCAACATAATGG |
NGG |
2 |
0.4006 |
Tier III |
| 11 |
NC_000004.12 |
4 |
19830531 |
- |
LOC105374511 |
NNNTTTCAACAACATAATGG |
NGG |
2 |
0.3117 |
Tier III |
| 12 |
NC_000004.12 |
4 |
145497507 |
- |
SMAD1-AS2 |
NNNTATCACCAACATAAAGG |
NGC |
2 |
0.0148 |
Tier III |
| 13 |
NC_000004.12 |
4 |
23755870 |
- |
LOC105374528 |
NNNTATCACCAACATAGTGG |
NTG |
2 |
0.0069 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
154596153 |
- |
Cacna1e |
NNNTATGACTAACATAATGG |
NGG |
2 |
0.4429 |
Tier II |
| 2 |
NC_000076.6 |
10 |
42368026 |
- |
Afg1l |
NNNTCTCACCAACATAAAGG |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000081.6 |
15 |
33486041 |
+ |
Cpq |
NNNTATCTCCAGCATAATGG |
NGG |
2 |
0.3095 |
Tier II |
| 4 |
NC_000081.6 |
15 |
4956058 |
- |
Mroh2b |
NNNTATCACCAACATAATGC |
NAG |
2 |
0.1111 |
Tier II |
| 5 |
NC_000073.6 |
7 |
140885267 |
- |
Psmd13 |
NNNTATCACCCACATGATGG |
NGG |
2 |
0.0769 |
Tier II |
| 6 |
NC_000068.7 |
2 |
169961697 |
- |
Tshz2 |
NNNTATCATCAACATAATGG |
NGA |
2 |
0.0608 |
Tier II |
| 7 |
NC_000080.6 |
14 |
29493631 |
+ |
Cacna2d3 |
NNNTGTCACCAACATAATGG |
NGA |
2 |
0.05 |
Tier II |
| 8 |
NC_000086.7 |
X |
120470291 |
+ |
Pcdh11x |
NNNTATCACCAACAGAATAG |
NGG |
2 |
0.0357 |
Tier II |
| 9 |
NC_000082.6 |
16 |
9627222 |
+ |
Grin2a |
NNNTATCACCAACATAAGGG |
NGA |
2 |
0.0231 |
Tier II |
| 10 |
NC_000072.6 |
6 |
43340856 |
- |
Tpk1 |
NNNTATCTCCAACATAATGG |
NGC |
2 |
0.0095 |
Tier II |
| 11 |
NC_000078.6 |
12 |
98548206 |
- |
Kcnk10 |
NNNTATCACCACCATAATGG |
NGT |
2 |
0.0042 |
Tier II |
| 12 |
NC_000068.7 |
2 |
52954604 |
- |
Fmnl2 |
NNNTATCACCACCATCATGG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000075.6 |
9 |
35293977 |
- |
Gm33838 |
NNNTATAAGCAACATAATGG |
NGG |
2 |
0.4643 |
Tier III |
| 14 |
NC_000077.6 |
11 |
44092354 |
+ |
Gm12153 |
NNNTATCACCAACACAATGT |
NGG |
2 |
0.1909 |
Tier III |
| 15 |
NC_000068.7 |
2 |
5101430 |
+ |
Gm39755 |
NNNTATGACCCACATAATGG |
NGG |
2 |
0.1882 |
Tier III |
Other clones with same target sequence:
(none)
