Construct: sgRNA BRDN0001149260

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATACCTTATGGGAATAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK6 (5597)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76398

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 52047004 - MAPK6 NNNACCTTATGGGAATAATG NGG 0 1.0 Tier I
2 NC_000009.12 9 74609061 - RORB NNNACAGTATGGGAATAATG NGG 2 0.5462 Tier II
3 NC_000017.11 17 48609994 + HOXB7 NNNTCCTGATGGGAATAATG NGG 2 0.4667 Tier II
4 NC_000014.9 14 90259577 + PSMC1 NNNACCTTATTGGAATAATA NGG 2 0.3606 Tier II
5 NC_000014.9 14 99600271 + CCDC85C NNNACCTTGTGTGAATAATG NGG 2 0.2564 Tier II
6 NC_000006.12 6 70579914 + SDHAF4 NNNATCTTATGTGAATAATG NGG 2 0.2448 Tier II
7 NC_000008.11 8 72673137 - KCNB2 NNNACCTTTTGTGAATAATG NGG 2 0.2308 Tier II
8 NC_000011.10 11 72854296 - FCHSD2 NNNACCTTTTGTGAATAATG NGG 2 0.2308 Tier II
9 NC_000005.10 5 172181347 - STK10 NNNGCCTTATGGGAAGAATG NGG 2 0.2163 Tier II
10 NC_000012.12 12 24448758 + SOX5 NNNACGTTATTGGAATAATG NGG 2 0.1923 Tier II
11 NC_000003.12 3 100853735 - ABI3BP NNNGCCTTATGGGAATAACG NGG 2 0.1724 Tier II
12 NC_000001.11 1 227260169 - CDC42BPA NNNACCTTTTGGGAATAAAG NGG 2 0.1714 Tier II
13 NC_000023.11 X 104684632 + IL1RAPL2 NNNACCTTATGGGAATAAAG NAG 2 0.0741 Tier II
14 NC_000021.9 21 22437899 + MAPK6P2 NNNTCCTTATGGGAATAATG NGG 1 0.6364 Tier III
15 NC_000004.12 4 43476161 - LINC02383 NNNACCTTAAGGGAATATTG NGG 2 0.4286 Tier III
16 NC_000015.10 15 96148141 - NR2F2-AS1 NNNGCCTTATGGGAACAATG NGG 2 0.4167 Tier III
17 NC_000015.10 15 96148141 - LOC112268156 NNNGCCTTATGGGAACAATG NGG 2 0.4167 Tier III
18 NC_000015.10 15 96148141 - LOC124903583 NNNGCCTTATGGGAACAATG NGG 2 0.4167 Tier III
19 NC_000008.11 8 53537602 - MAPK6P1 NNNTGCTTATGGGAATAATG NGG 2 0.3818 Tier III
20 NC_000014.9 14 83913081 - LINC02305 NNNACCTTATGGGAATAAAA NGG 2 0.2679 Tier III
21 NC_000013.11 13 46209669 - LOC107984578 NNNACCTTATGGAAATAATG NAG 2 0.2393 Tier III
22 NC_000022.11 22 47479587 - LINC01644 NNNACCTTATGGGAAAAAGG NGG 2 0.2273 Tier III
23 NC_000002.12 2 34066367 + LINC01317 NNNCCCTTATGGGAATAAAG NGG 2 0.1008 Tier III
24 NC_000002.12 2 34066367 + LOC105374456 NNNCCCTTATGGGAATAAAG NGG 2 0.1008 Tier III
25 NC_000008.11 8 46974082 + MAPK6P4 NNNCCCTTATGGGAATAATG NGT 2 0.0057 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 75397591 + Mapk6 NNNACCTTATGGGAATAATG NGG 0 1.0 Tier I
2 NC_000084.6 18 80808624 + Atp9b NNNACCTTATGGGAATGATG NAG 2 0.0458 Tier I
3 NC_000077.6 11 88135887 - Cuedc1 NNNACCTTAAAGGAATAATG NGG 2 0.8571 Tier II
4 NC_000077.6 11 96287650 - Hoxb7 NNNTCCTGATGGGAATAATG NGG 2 0.4667 Tier II
5 NC_000074.6 8 127067471 + Pard3 NNNACCTTATGGGGAAAATG NGG 2 0.4242 Tier II
6 NC_000068.7 2 174601641 + Zfp831 NNNACTTTATGGGAAGAATG NGG 2 0.3214 Tier II
7 NC_000070.6 4 139273685 - Capzb NNNACCTTATAGGAATAAGG NGG 2 0.25 Tier II
8 NC_000070.6 4 112953459 - Skint6 NNNACCATATGGGAATAATG NAG 2 0.2269 Tier II
9 NC_000070.6 4 113672258 - Skint5 NNNACCATATGGGAATAATG NAG 2 0.2269 Tier II
10 NC_000067.6 1 15440964 - Kcnb2 NNNACCTTATGGGAAGAATG NAG 2 0.0897 Tier II
11 NC_000067.6 1 15440964 - Gm51415 NNNACCTTATGGGAAGAATG NAG 2 0.0897 Tier II
12 NC_000070.6 4 48560107 + Msantd3 NNNACCTTAGGGGAATAATG NGA 2 0.0347 Tier II
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Other clones with same target sequence:

(none)