Construct: sgRNA BRDN0001149263
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTGAACTACAACGTAACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PKD1 (5310)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76843
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
2112915 |
- |
PKD1 |
NNNGAACTACAACGTAACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
14924026 |
+ |
PKD1P3 |
NNNGAACTACAACATCACCG |
NGG |
2 |
0.0 |
Tier III |
| 3 |
NC_000016.10 |
16 |
14924026 |
+ |
PKD1P3-NPIPA1 |
NNNGAACTACAACATCACCG |
NGG |
2 |
0.0 |
Tier III |
| 4 |
NC_000016.10 |
16 |
16322677 |
+ |
PKD1P1 |
NNNGAACTACAACATCACCG |
NGG |
2 |
0.0 |
Tier III |
| 5 |
NC_000016.10 |
16 |
16322677 |
+ |
LOC131696449 |
NNNGAACTACAACATCACCG |
NGG |
2 |
0.0 |
Tier III |
| 6 |
NC_000016.10 |
16 |
16362696 |
+ |
PKD1P2 |
NNNGAACTACAACATCACCG |
NGG |
2 |
0.0 |
Tier III |
| 7 |
NC_000016.10 |
16 |
18345991 |
- |
PKD1P4 |
NNNGAACTACAACATCACCG |
NGG |
2 |
0.0 |
Tier III |
| 8 |
NC_000016.10 |
16 |
18345991 |
- |
PKD1P4-NPIPA8 |
NNNGAACTACAACATCACCG |
NGG |
2 |
0.0 |
Tier III |
| 9 |
NC_000016.10 |
16 |
18389486 |
- |
PKD1P5 |
NNNGAACTACAACATCACCG |
NGG |
2 |
0.0 |
Tier III |
| 10 |
NC_000016.10 |
16 |
18389486 |
- |
PKD1P5-LOC105376752 |
NNNGAACTACAACATCACCG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
73384346 |
+ |
Gpr155 |
NNNGAACAACAATGTAACCG |
NGG |
2 |
0.56 |
Tier II |
Other clones with same target sequence:
(none)
