Construct: sgRNA BRDN0001149264
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGCTAAACCAGCTGACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CIT (11113)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76722
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
119735321 |
- |
CIT |
NNNGCTAAACCAGCTGACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
61446342 |
- |
PRKCH |
NNNGCTAAACCACCTGACTG |
NGG |
2 |
0.1943 |
Tier II |
| 3 |
NC_000016.10 |
16 |
85649163 |
- |
GSE1 |
NNNGCTAAACCAGCTGACTG |
NTG |
2 |
0.018 |
Tier II |
| 4 |
NC_000009.12 |
9 |
85635682 |
+ |
AGTPBP1 |
NNNGCTAAACCAGGTGACCT |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
115967925 |
+ |
Cit |
NNNGCTGAACCAGCTCACCG |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000078.6 |
12 |
17322020 |
+ |
Atp6v1c2 |
NNNGCTAGACCAGCTGACCA |
NGG |
2 |
0.6875 |
Tier II |
| 3 |
NC_000080.6 |
14 |
29579428 |
- |
Cacna2d3 |
NNNAATAAACCAGCTGACCG |
NGG |
2 |
0.5143 |
Tier II |
| 4 |
NC_000067.6 |
1 |
91317914 |
+ |
Scly |
NNNGCTAAACAAGCTGGCCG |
NGG |
2 |
0.1324 |
Tier II |
| 5 |
NC_000081.6 |
15 |
74670348 |
- |
Arc |
NNNGCTAAGCCAGCTCACCG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
